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SN/T 1151.5-2015 English PDF

SN/T 1151.5: Historical versions

Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
SN/T 1151.5-2015RFQ ASK 3 days (Technical specifications of shrimp baculovirus disease quarantine) Valid
SN/T 1151.5-2014399 Add to Cart 3 days Quarantine protocol for tetrahedral baculovirosis Obsolete
SN/T 1151.5-2003279 Add to Cart 3 days The shrimp baculovirus (BP) detection method Obsolete

Similar standards

SC/T 4030   GB 5009.261   SN/T 1151.3   SN/T 1151.2   SN/T 1151.1   

Basic data

Standard ID: SN/T 1151.5-2015 (SN/T1151.5-2015)
Description (Translated English): (Technical specifications of shrimp baculovirus disease quarantine)
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B51
Classification of International Standard: 67.120.30
Date of Issue: 2015-02-09
Date of Implementation: 2015-09-01
Older Standard (superseded by this standard): SN/T 1151.5-2003
Regulation (derived from): State-Quality-Inspection-Accreditation [2015] 59
Issuing agency(ies): General Administration of Customs

SN/T 1151.5-2014: Quarantine protocol for tetrahedral baculovirosis

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Technical specifications shrimp baculovirus disease quarantine) People's Republic of China Entry-Exit Inspection and Quarantine Standards Instead of the SN/T 1151.5-2003 Technical specifications prawn baculovirus disease quarantine Issued on. 2014-11-19 2015-05-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

SN/T 1151 is divided into six sections. --- Taura Syndrome shrimp quarantine technical specifications; --- White spot disease quarantine technical specifications; --- Monodon baculovirus (MBV) diagnostic methods; --- Shrimp yellow head disease quarantine technical specifications; --- Prawn baculovirus disease quarantine technical specifications; --- White Spot virus dot blot and in situ hybridization procedures. This section SN/T 1151 Part 5. This section drafted in accordance with GB/T 1.1-2009 given rules. The partial replacement of SN/T 1151.5-2003 "shrimp baculovirus (BP) detection method." This section compared with the SN/T 1151.5-2003, the main technical changes are as follows. --- Increased sampling; --- Primers for PCR test has been modified; --- Amplified product increases the reference sequence. This section proposed and managed by the National Certification and Accreditation Administration Committee. This section was drafted by. People's Republic of China, Shenzhen CIQ, Shenzhen Inspection and Quarantine Science Research Institute, the Chinese people Republic of Shandong CIQ. The main drafters of this section. Shixiu Jie, He Junjiang, Jiang Yuting Wang Jinjin, in force, Zhengxiao Cong, Jia Peng, blue Wensheng, Yang Jinshun, Liu Orientin. This part of the standard replaces the previous editions are. --- SN/T 1151.5-2003. Technical specifications prawn baculovirus disease quarantine

1 Scope

SN/T 1151 to the present standard specifies the shrimp baculovirus microscopy, polymerase chain reaction detection method. This standard applies to prawn baculovirus disease epidemiology, diagnosis, quarantine and monitoring.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 18088 Entry and Exit Animal Quarantine sampling GB 19489 General requirements for laboratory biosafety

3 Reagents and materials

3.1 Water. in line with GB/T 6682 a water specifications. 3.2 TaqDNA polymerase. -20 ℃ to save, not to repeated freezing and thawing or extreme temperature changes. 3.3 dNTPs. containing dCTP, dGTP, dATP, dTTP each 10mmol/L. 3.4 primer. a pair of primers, concentration of 40μmol/L, the sequence is. 6581F. 5'-TGT-AGC-AGC-AGA-GAA-GAG-3 ' 6582R. 5'-CAC-TAA-GCC-TAT-CTC-CAG-3 ' Amplification of viral genes in the DNA fragment of 645bp. 3.5 ethanol. AR, before using the pre-cooled to -20 ℃.

4 instruments and equipment

4.1 scissors and tweezers. 4.2 slides and cover slips. 4.3 incubator. 4.4 ordinary refrigerator and ultra-low temperature freezer. 4.5 upright microscopes. 4.6 centrifuges and centrifuge tubes. 4.7 micropipette and tips. 4.8 PCR amplification. 4.9 electrophoresis. 4.10 UV lamp observation or gel imager.
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