SN/T 1151.1-2011 English PDF

US$259.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
SN/T 1151.1-2011: Quarantine protocol for shrimp taura syndrome
Status: Valid

SN/T 1151.1: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1151.1-2011	259	Add to Cart	3 days	Quarantine protocol for shrimp taura syndrome	Valid
SN/T 1151.1-2002	239	Add to Cart	2 days	Test method of reverse transcription polymerase chain reaction (RT-PCR) for tuara syndrome virus (TSV)	Obsolete

Similar standards

GB 5009.261   SN/T 1151.5   SN/T 1151.3   SN/T 1151.2   

Basic data

Standard ID: SN/T 1151.1-2011 (SN/T1151.1-2011)
Description (Translated English): Quarantine protocol for shrimp taura syndrome
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B50
Classification of International Standard: 67.120.30
Word Count Estimation: 10,116
Date of Issue: 2011-05-31
Date of Implementation: 2011-12-01
Older Standard (superseded by this standard): SN/T 1151.1-2002
Quoted Standard: GB/T 18088
Adopted Standard: OIE Aquatic Animal Disease Diagnostic Manual 2009, MOD
Regulation (derived from): National-Quality-Inspection-accreditation (2011) 291
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the method of operation of shrimp Taura syndrome RT-PCR and Real-time RT-PCR detection. This section applies to the epidemiological investigation of shrimp Taura syndrome, diagnosis, quarantine and monitoring.

SN/T 1151.1-2011: Quarantine protocol for shrimp taura syndrome

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for shrimp taura syndrome People's Republic of China Entry-Exit Inspection and Quarantine Standards Instead of the SN/T 1151.1-2002 Taura Syndrome shrimp quarantine Technical Specifications Issued on. 2011-05-31 2011-12-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

SN/T 1151 series of standards is divided into six parts. --- Taura Syndrome shrimp quarantine technical specifications; --- White spot disease quarantine technical specifications; --- Monodon baculovirus (MBV) diagnostic methods; --- Shrimp yellow head disease quarantine technical specifications; --- Shrimp baculovirus (BP) test methods; --- White Spot virus dot blot and in situ hybridization procedures. This section SN/T 1151 Part 1. This section drafted in accordance with GB/T 1.1-2009 given rules. The partial replacement of SN/T 1151.1-2002 "shrimp Taura syndrome virus (TSV) reverse transcription polymerase chain reaction (RT- PCR) diagnostic methods. " This section compared with the SN/T 1151.1-2002, major technical changes as follows. --- Integration and optimization of RT-PCR detection method; --- New Real-timeRT-PCR detection method. The partial modification using World Organisation for Animal Health (OIE) of "aquatic animal disease diagnostic manual" (2009 Edition) chapter 2.2.4, and Taura Syndrome shrimp by RT-PCR method for the integration and optimization. This section proposed and managed by the National Certification and Accreditation Administration Committee. This section was drafted by. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau, Shenzhen People's Republic of China Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Fujian Exit Inspection and Quarantine. The main drafters of this section. Xiong Wei, Zhang Qiang, Liu Orientin, Cheng Tang, Jing Jiang, Li Jian, Qiu Lu, Wang Qiao whole, Huang Zhongrong, Hu Yongqiang. This part of the standard replaces the previous editions are. --- SN/T 1151.1-2002. Taura Syndrome shrimp quarantine Technical Specifications

1 Scope

SN/T 1151 This section specifies the method of operation of shrimp Taura syndrome RT-PCR and Real-timeRT-PCR detection. This section applies to epidemiology, diagnosis, and monitoring of quarantine shrimp Taura syndrome.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB/T 18088 Entry and Exit Animal Quarantine sampling

3 Overview

Taura syndrome (Taurasyndrome) is an infection by the Taura syndrome virus (Taurasyndromevirus, TSV) caused A serious threat to shrimp farming important disease, which is the World Organisation for Animal Health (OIE) prescribed notifiable aquatic animal diseases. TSV few It can infect virtually all types of farmed shrimp, such as. South American white shrimp, red forehead shrimp, brown shrimp, white shrimp, black tiger shrimp, etc., and the incubation period of the infection Shrimp, its death rate. TSV infection has three distinct phases. acute, transition and chronic phase. In the acute phase, shrimp skin epithelium Tissue sections can be seen in the typical pathological lesions. In the transition period and the chronic phase, but not typical pathological injury; shrimp main incidence of death To occur in the acute phase, the survivors after acute infection after a brief transition period after entering a long period of chronic infection, in a slow sexy Transfection of lymphoid organs shrimp in their lifetime presence of the virus, the virus carrying the shrimp can spread the virus to other susceptible shrimp level. disease Toxicity studies confirm, TSV is a small icosahedral non-enveloped viruses, the diameter of 32nm, its genome is a single positive-strand RNA by the 10205 Nucleotides, containing two large open reading frame (Openreadframe, ORF), ORF1 encodes non-structural proteins such as helicase, Protease and RNA-dependent RNA polymerase, etc., ORF2 encoding structural proteins such as capsid protein VP1, VP2, VP3 (large molecular weight Small were 55kDa, 40kDa, 24kDa).

4 Reagents and materials

4.1 Taq Taq polymerase enzyme 10-fold concentrated buffer. Taq enzyme concentration 5U/μL, -20 ℃, avoid repeated freezing and thawing. 4.2 retroviral reverse transcriptase enzyme 10-fold concentrated buffer. reverse transcriptase concentration of 50U/μL, -20 ℃, avoid repeated freezing and thawing. 4.3 RNA inhibitors. a concentration of 40U/μL, -20 ℃, avoid repeated freezing and thawing. 4.4 dNTP. containing dATP, dGTP, dCTP, dTTP each 10mmol/L, -20 ℃ preservation. 4.5 RT-PCR primer. primer concentration were 20μmol/L; there are two pairs of primers can optionally use one; as primers 9992F and 9195R OIE Recommended primers amplified gene fragment 231 bp in size, gene amplification primers TSVF TSVR and size of 295bp, Its sequence is as follows. The upstream primer (9992F). 5'-AAG-TAG-ACA-GCC-GCG-CTT-3 ' Downstream primer (9195R). 5'-TCA-ATG-AGA-GCT-TGG-TCC-3 ' The upstream primer (TSVF). 5'-ATT-GAA-TAC-TTA-GCA-CAG-CGA-CC-3 ' Downstream primer (TSVR). 5'-GAA-CAC-CAC-TAC-CAT-AGG-GGA-G-3 ' ......

Image     

Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of SN/T 1151.1-2011_English be delivered?

Answer: Upon your order, we will start to translate SN/T 1151.1-2011_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of SN/T 1151.1-2011_English with my colleagues?

Answer: Yes. The purchased PDF of SN/T 1151.1-2011_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.

Question 5: Should I purchase the latest version SN/T 1151.1-2011?

Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version SN/T 1151.1-2011 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically.