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SN/T 1151.4-2011 English PDF

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SN/T 1151.4-2011: Quarantine protocol for shrimp yellow head disease
Status: Valid

SN/T 1151.4: Historical versions

Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
SN/T 1151.4-2011319 Add to Cart 3 days Quarantine protocol for shrimp yellow head disease Valid
SN/T 1151.4-2003359 Add to Cart 3 days Protocol of reverse transcription polymerase chain reaction (RT-PCR) for yellowhead virus of shrimp (YHV) Obsolete

Similar standards

GB 5009.261   SN/T 1151.5   SN/T 1151.3   SN/T 1151.1   

Basic data

Standard ID: SN/T 1151.4-2011 (SN/T1151.4-2011)
Description (Translated English): Quarantine protocol for shrimp yellow head disease
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B50
Classification of International Standard: 67.120.30
Word Count Estimation: 12,196
Date of Issue: 2011-05-31
Date of Implementation: 2011-12-01
Older Standard (superseded by this standard): SN/T 1151.4-2003
Quoted Standard: GB/T 18088
Adopted Standard: OIE Aquatic Animal Disease Diagnostic Manual 2009, MOD
Regulation (derived from): National-Quality-Inspection-accreditation (2011) 291
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the method of operation of shrimp yellow head disease RT-PCR and Real-time RT-PCR detection. This section applies to shrimp yellow head disease epidemiology, diagnosis, quarantine and monitoring.

SN/T 1151.4-2011: Quarantine protocol for shrimp yellow head disease

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for shrimp yellow head disease People's Republic of China Entry-Exit Inspection and Quarantine Standards Instead of the SN/T 1151.4-2003 Shrimp yellow head disease quarantine Technical Specifications Issued on. 2011-05-31 2011-12-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

SN/T 1151 series of standards is divided into six parts. --- Taura Syndrome shrimp quarantine technical specifications; --- White spot disease quarantine technical specifications; --- Monodon baculovirus (MBV) diagnostic methods; --- Shrimp yellow head disease quarantine technical specifications; --- Shrimp baculovirus (BP) test methods; --- White Spot virus dot blot and in situ hybridization procedures. This section SN/T 1151 Part 4. This section drafted in accordance with GB/T 1.1-2009 given rules. The partial replacement of SN/T 1151.4-2003 "shrimp yellow head virus (YHV) reverse transcription polymerase chain reaction (RT-PCR) detection side law". This section compared with the SN/T 1151.4, the main technical changes are as follows. --- Add nested RT-PCR method for the differential diagnosis; --- New Real-timeRT-PCR detection method. The partial modification using World Organisation for Animal Health (OIE) of "aquatic animal disease diagnostic manual" (2009 edition) 2.2.7 chapter. This section proposed and managed by the National Certification and Accreditation Administration Committee. This section was drafted by. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau, Shenzhen People's Republic of China Entry-Exit Inspection and Quarantine Bureau. The main drafters of this section. Xiong Wei, Jiang Jing, Zhang Qiang, Liu Orientin, Li Jian, Wang Qiao whole, Qiu Lu, Huang Zhongrong, Hu Yongqiang. This part of the standard replaces the previous editions are. --- SN/T 1151.4-2003. Shrimp yellow head disease quarantine Technical Specifications

1 Scope

SN/T 1151 This section specifies the method of operation of shrimp yellow head disease RT-PCR and Real-timeRT-PCR detection. This section applies to shrimp yellow head disease epidemiology, diagnosis, quarantine and monitoring.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB/T 18088 Entry and Exit Animal Quarantine sampling

3 Overview

Shrimp yellow head disease (Yelowheaddisease, YHD) is a shrimp by the yellow head virus (Yelowheadvirus, YHV) caused serious Threat of shrimp diseases, widespread infection, death rate, the World Organisation for Animal Health (OIE) prescribed notifiable aquatic animal diseases. Currently, only South Penaeus vannamei outbreaks YHD outbreaks reported, but the Japanese shrimp, white shrimp, krill, also can Naturally infected with YHV. YHV belong to the yellow head syndrome virus (Yelowheadcomplexofviruses), the group currently has six virus Known genotype, YHV (genotype 1) is one of a type, and is the only pathogen yellow head disease. Gill associated virus (Gil- associatedvirus, GAV) belongs to genotype 2. GAV and several other type (type genotype 3 to 6) in East Africa, Asia and Australia Health monodon are more common, but rarely cause disease. YHV is enveloped baculovirus whose genome is a single positive-stranded RNA.

4 Reagents and materials

4.1 Taq Taq DNA polymerase enzyme 10-fold reaction buffer. Taq enzyme concentration 5U/μL, -20 ℃, avoid repeated freezing and thawing. 4.2 10 times reverse transcriptase enzyme reverse transcriptase reaction buffer. reverse transcriptase concentration of 50U/μL, -20 ℃, avoid repeated freezing and thawing. 4.3 RNA inhibitor (40U/μL). - 20 ℃, avoid repeated freezing and thawing. 4.4 dNTP. containing dATP, dGTP, dCTP, dTTP each 10mmol/L, -20 ℃ preservation. 4.5 RT-PCR primers were. concentration 20μmol/L, which sequence is as follows. Primer (10F). 5'-CCG-CTA-ATT-TCA-AAA-ACT-ACG-3 '; Primer (144R). 5'-AAG-GTG-TTA-TGT-CGA-GGA-AGT-3 '; Primer (GY1). 5'-GAC-ATC-ACT-CCA-GAC-AAC-ATC-TG-3 '; Primer (GY2). 5'-CAT-CTG-TCC-AGA-AGG-CGT-CTA-TGA-3 '; Primer (GY4). 5'-GTG-AAG-TCC-ATG-TGT-GTG-AGA-CG-3 '; Primer (GY5). 5'-GAG-CTG-GAA-TTC-AGT-GAG-AGA-ACA-3 '; Primer (Y3). 5'-ACG-CTC-TGT-GAC-AAG-CAT-GAA-GTT-3 '; Primer (G6). 5'-GTA-GTA-GAG-ACG-AGT-GAC-ACC-TAT-3 '; Primer (YC-F1ab). 5'-ATC-GTC-GTC-AGC-TAC-CGC-AAT-ACT-GC-3 'and 5'-ATC-GTC-GTC-AGY-TAY-CGT-AAC-ACC-GC-3 'mixing the two primers;
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