SN/T 1146.2-2017 English PDF

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SN/T 1146.2-2017: Molecular detection of Tobacco ringspot virus
Status: Valid

SN/T 1146.2: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1146.2-2017	279	Add to Cart	3 days	Molecular detection of Tobacco ringspot virus	Valid
SN/T 1146.2-2009	479	Add to Cart	3 days	Molecular detection of Tobacco ringspot virus	Obsolete

Similar standards

GB/T 12729.2   SN/T 0803.6   GB 2635   

Basic data

Standard ID: SN/T 1146.2-2017 (SN/T1146.2-2017)
Description (Translated English): Molecular detection of Tobacco ringspot virus
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B35
Word Count Estimation: 12,177
Date of Issue: 2017-07-21
Date of Implementation: 2018-03-01
Older Standard (superseded by this standard): SN/T 1146.2-2009
Quoted Standard: GB/T 6682; SN/T 1146
Regulation (derived from): State-Quality-Inspection-Accreditation (2017) 337
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the basic principles and methods for molecular biology testing of tobacco ring spot virus. This section applies to the detection of tobacco ring spot virus carried in asexual propagation materials, tissue culture seedlings, bulbs and seedlings.

SN/T 1146.2-2017: Molecular detection of Tobacco ringspot virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Molecular detection of Tobacco ringspot virus People's Republic of China entry and exit inspection and quarantine industry standards Replace SN/T 1146.2-2009 Tobacco ring spot virus molecular biology detection method Released on.2017-07-21 2018-03-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

SN/T 1146 is divided into 3 parts. --- Part 1. Quarantine and identification methods for quarantine tobacco ring spot virus; --- Part 2. Molecular biological detection methods for tobacco ring spot virus; --- Part 3. Quarantine and identification methods for tobacco ring spot virus. This part is the second part of SN/T 1146. This part is drafted in accordance with the rules given in GB/T 1.1-2009. This part replaces SN/T 1146.2-2009 "Tobacco ring spot virus molecular biology detection method". The main technical differences between this part and SN/T 1146.2-2009 are as follows. --- Appendix C nucleic acid extraction increased magnetic beads adsorption method and commercial kit method; ---PCR primers have been adjusted; --- Increased RT-LAMP detection method; --- Increased preparation method of buffer; --- Deleted the basic information of Appendix A tobacco ring spot virus. This part is proposed and managed by the National Certification and Accreditation Administration. This section drafted by. Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Beijing Entry-Exit Inspection and Quarantine Bureau, China Academy of Inspection and Quarantine. The main drafters of this section. Yang Cuiyun, Yu Cui, Zhang Luzhen, Hu Peilong, Jiao Binbin, Deng Congliang, Wei Meisheng, Gao Hui, Qiao Yanyan. The previous versions of the standards replaced by this section are. ---SN/T 1146.2-2009. Tobacco ring spot virus molecular biology detection method

1 Scope

This part of SN/T 1146 specifies the basic principles and methods for molecular biology testing of tobacco ring spot virus. This section applies to the detection of tobacco ring spot virus carried in asexual propagation materials, tissue culture seedlings, bulbs and seedlings.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods SN/T 1146 Tobacco ring spot virus quarantine and identification method

3 Tobacco ring spot virus basic information

Chinese name. Tobacco ring spot virus English name. Tobaccoringspotvirus Abbreviation. TRSV Taxonomic status. Comoviridae, nepovirus. Route of transmission. The virus can be transmitted by seed, grafting, mechanical inoculation and mediators. S. cerevisiae (Xiphinemaamericanum); soybean, melon, lettuce, cowpea, amaranth, potato and geranium can be planted, soybean seed rate Up to 40% to 100%. Genome. The viral genome contains two ssRNAs. RNA-1 is 7.514 kb in length and RNA-2 is 3.929 kb in length, coat protein gene 1.548kb.

4 Principle of the method

The serological and molecular biological characteristics of tobacco ring spot virus are the main basis for quarantine identification of the virus. Using RT-PCR, IC-RT- The detection of the virus was carried out by molecular biological methods such as PCR, real-time fluorescent RT-PCR and RT-LAMP. 5 instruments, utensils and reagents 5.1 Instrumentation PCR instrument, real-time PCR instrument, ultra-clean bench, electronic balance (1/10000g), electrophoresis, electrophoresis tank, gel imaging system System, water bath, high speed refrigerated centrifuge, refrigerator, -80 ° C ultra-low temperature refrigerator, autoclave, ice machine, microwave oven, vortex shaker, pH Count. 5.2 Appliances Adjustable micropipettes (2μL, 10μL, 100μL,.200μL, 1000μL, 5000μL) and corresponding RNase-free tips, no ......

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