SN/T 1146-2010 English PDF

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1146-2010	359	Add to Cart	3 days	Detection and identification of tobacco ringspot virus	Obsolete
SN/T 1146-2002	359	Add to Cart	3 days	Plant quarantine. Methods for inspection and identification of tobacco ringspot Nepovirus	Obsolete

Similar standards

NY/T 2288   SN/T 1204   NY 39   SN/T 1144   SN/T 1147   SN/T 1146.2   

Basic data

Standard ID: SN/T 1146-2010 (SN/T1146-2010)
Description (Translated English): Detection and identification of tobacco ringspot virus
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 07.080
Word Count Estimation: 9,921
Date of Issue: 2010-05-27
Date of Implementation: 2010-12-01
Older Standard (superseded by this standard): SN/T 1146-2002
Quoted Standard: SN/T 1840; SN/T 2122
Regulation (derived from): National Quality Inspection (2010) 290; industry standard filing Notice 2010 No. 10 (No. 130 overall)
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the entry and exit of the tobacco plant quarantine in quarantine ringspot virus identification methods. This standard applies to entry and exit of plants and their products, tobacco ringspot virus quarantine identification.

SN/T 1146-2010: Detection and identification of tobacco ringspot virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of tobacco ringspot virus People's Republic of China Entry-Exit Inspection and Quarantine Standards Instead of the SN/T 1146-2002 Tobacco ringspot virus quarantine and identification methods Issued on. 2010-05-27 2010-12-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Instead of the standard SN/T 1146-2002 "tobacco ringspot virus quarantine and identification methods." This standard compared with SN/T 1146-2002, the main technical changes are as follows. --- Quarantine sampling site according to SN/T 2122-2008 "entry and exit quarantine of plants and plant products sampling" execution (see 6.2); --- Remove the original standards Appendix A; --- Added "symptom checker" one (see 6.3); --- Immune electron microscopy method according to SN/T 1840-2006 "plant virus immune electron microscopy detection method" standard (see 7.3). This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China, Shenzhen CIQ. The main drafters of this standard. Chen Chi-nan, Tang Qiong, Cheng Yun Ling apricot, Zheng Wenhua, Wang Dongyong. This standard replaces the standards previously issued as follows. --- SN/T 1146-2002. Tobacco ringspot virus quarantine and identification methods

1 Scope

This standard specifies the export plant quarantine of tobacco ringspot virus in quarantine and identification methods. This standard applies to the entry and exit of plants and their products in quarantine and identification of tobacco ringspot virus.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member, undated references, the latest edition (including any amendments) applies to this document. SN/T 1840 plant virus immune electron microscopy method SN/T 2122 entry and exit quarantine of plants and plant products sampling rules Principle 3 Tobacco ringspot virus (tobaccoringspotvirus, referred TRSV), iso-called Anemone necrosis virus (anemonenecrosis virus), blueberry necrotic ringspot virus (blueberrynecroticringspotvirus), tobacco ringspot virus No. 1 (tobaccoringspot virus1). The virus is an isometric polyhedral spherical virus with a diameter of 25nm ~ 29nm, nematodes pass polyhedron virus is a member, can infect 54 Section 300 kinds of plants, the main cash crops are beans, potato, sweet potato, tobacco, watermelon, cucumbers, melons, carrots, Tangchang Po, lily, iris, Prunus, apples, grapes, sweet cherries. Symptoms of plant infection by TRSV vary by host, usually appear blade ring Or linear pattern, mottled or chlorotic spots, necrotic spots; root rot, stem dieback, are the fruit of malformations. The virus mainly rely on nematodes and seeds Spread, mainly in North America, Oceania, Europe, Africa and other regions, with a broad host range, symptoms harm, immunogenicity, easy to make Preparation of high titer, specifically the strong resistance of the virus antisera and other characteristics (see Appendix A). Serologically double antibody sandwich (DAS-ELISA) binding Identification of host biological identification method or immune electron microscopy, as identified in the virus quarantine work basis. 4 utensils, equipment, facilities Mortar, micro juicer, microplate reader, low-speed centrifuge (speed of 4000r/min), bio-incubator (with light, adjustable temperature range Wai is 0 ℃ ~ 50 ℃), ELISA plate (48 or 96 wells), Eppendorf tubes (volume of 1.5mL), single channel pipette (10μL, 100 μL, 1000μL), 8-channel pipette (100μL or 200μL), heated water bath or incubator, analyze electronic balance, pH meter, white Plate (40cm × 50cm), blunt forceps, quarantine temperature (net) chamber.

5 Reagents

5.1 10 × PBST buffer Sodium chloride (NaCl) 80g, potassium dihydrogen phosphate (KH2PO4) 2g, disodium hydrogen phosphate (Na2HPO4) 11.5g, potassium chloride (KCl) 2g, Tween -20 (Tween-20) 5mL dissolved in distilled water, dilute to 1000mL. 5.2 Sample Extraction buffer Sodium sulfite (Na2SO3) 1.3g, polyvinyl pyrrolidone (MW24000 ~ 40000, PVP) 20g, sodium azide (of NaN3) ......

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