LY/T 1772-2008 English PDF

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LY/T 1772-2008: Molecular identification method for varieties of poplar. Amplified fragment length polymorphism(AFLP)

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Basic data

Standard ID: LY/T 1772-2008 (LY/T1772-2008)
Description (Translated English): Molecular identification method for varieties of poplar. Amplified fragment length polymorphism(AFLP)
Sector / Industry: Forestry Industry Standard (Recommended)
Classification of Chinese Standard: B61
Classification of International Standard: 65.020
Word Count Estimation: 12,122
Date of Issue: 2008-09-03
Date of Implementation: 2008-12-01
Regulation (derived from): Industry standard filing Notice No. 10 of 2008
Issuing agency(ies): State Forestry Administration
Summary: This standard specifies the poplar fresh tissue (leaves, buds, etc.) for the material, the use of amplified fragment length polymorphism DNA technique (Amplified Fragment Length Polymorphism, referred AFLP) molecular identification of poplar varieties experimental method. This standard applies to molecular identification of poplar species.

LY/T 1772-2008: Molecular identification method for varieties of poplar. Amplified fragment length polymorphism(AFLP)

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Molecular identification method for varieties of poplar.Amplified fragment length polymorphism (AFLP) ICS 65.020 B61 People's Republic of China Forestry Industry Standard Experimental method for molecular identification of poplar varieties DNA amplified fragment length polymorphism (AFLP) Released on.2008-09-03 2008-12-01 implementation State Forestry Administration released

Foreword

Appendix A of this standard is an informative annex. This standard was proposed by the State Forestry Administration. This standard is under the jurisdiction of the National Forest Seed Standardization Technical Committee. This standard was drafted. Forestry Research Institute of Chinese Academy of Forestry, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Forest Bureau of Northern Forest Seed Testing Center. The main drafters of this standard. Li Jinhua, Zhang Weiwen, Wang Bin, Song Hongzhu, Zhou Chunjiang, Lu Mengzhu, Li Qingmei, Niu Zhengtian. Experimental method for molecular identification of poplar varieties DNA amplified fragment length polymorphism (AFLP)

1 Scope

This standard stipulates that the fresh tissue of poplar (leaf, bud, etc.) is used as the material, and the amplified fragment length polymorphism DNA method (Amplified) FragmentLengthPolymorphism (AFLP) is an experimental method for molecular identification of poplar varieties. This standard applies to the molecular identification of poplar varieties.

2 Principle

The primers matched by the adjacent cleavage sites are amplified, and finally the specific fragments are separated by denaturing polyacrylamide gel electrophoresis. Determine whether the tested variety and the control variety are the same by comparing the difference in DNA fingerprint band data between the tested variety and the control variety. Variety.

3 Instruments and equipment

3.1 General laboratory equipment. 3.2 Gradient PCR Amplifier. 3.3 Agarose gel electrophoresis system. 3.4 Denatured polyacrylamide gel electrophoresis system. 3.5 UV transilluminator. 3.6 Gel imaging system or camera system. 3.7 Heavy distilled water meter.

4 reagents and solutions

Analytically pure reagents were used in the analysis unless otherwise stated. The prepared solution is used after autoclaving. Water used in the experiment All are sterile ultrapure water. Store at -20 °C. 4.2 T犪狇DNA polymerase (1U/μL) and its 10×PCR reaction buffer 10×PCR reaction buffer containing.200 mmol/L Tris-HCl (pH 8.4), 15 mmol/L magnesium chloride (MgCl 2 ) and 500 mmol/L potassium chloride (KCl), stored at -20 °C. 4.3 T4 DNA ligase (1U/μL) and its 10× ligation reaction buffer 10× reaction buffer containing 350 mmol/LTris-HCl (pH 7.6), 50 mmol/L MgCl 2 and 500 mmol/L KCl and 5 mmol/L β-mercaptoethanol; stored at -20 °C. Store at -20 °C. λDNA and DNAMarker DL2000 were stored at -20 °C. ......

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