LY/T 1770-2008 English PDF

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LY/T 1770-2008: Tissue culture of eucalyptus clones

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Basic data

Standard ID: LY/T 1770-2008 (LY/T1770-2008)
Description (Translated English): Tissue culture of eucalyptus clones
Sector / Industry: Forestry Industry Standard (Recommended)
Classification of Chinese Standard: B64
Classification of International Standard: 65.020
Word Count Estimation: 18,152
Date of Issue: 2008-09-03
Date of Implementation: 2008-12-01
Quoted Standard: GB 6000; GB/T 6001; LY/T 1000
Regulation (derived from): Industry standard filing Notice No. 10 of 2008
Issuing agency(ies): State Forestry Administration
Summary: This standard specifies the tropical and subtropical eucalyptus clonal propagation of tissue culture seedlings of general principles, including the mother tree selection, explant acquisition and processing, media preparation, aseptic tissue culture transplants, seedlings management and Chupu, waste treatment and other aspects of the principles of the medium. This standard applies to plant tissue culture seedlings of Eucalyptus Clones Gui production, seedlings for reforestation and the establishment of Scion.

LY/T 1770-2008: Tissue culture of eucalyptus clones

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Tissue culture of eucalyptus clones ICS 65.020 B64 People's Republic of China Forestry Industry Standard Technical regulation of rapid propagation of eucalyptus clones Released on.2008-09-03 2008-12-01 implementation State Forestry Administration released

Content

Foreword III 1 Scope 1 2 Normative references 1 3 Terms and Definitions 1 4 Sources of propagation materials 2 5 Collection, disinfection and inoculation of explants 2 6 Preparation of medium 3 7 Inoculation 3 8 Proliferation culture, rooting culture and refining 4 9 Field transplanting and seedling management 5 10 seedlings out 6 11 Tissue Culture Management 6 Appendix A (informative appendix) Appendix B (informative appendix) Main characteristics of eucalyptus tissue culture seedlings and pests 8 Appendix C (informative appendix) Table 9 for job design Appendix D (informative) Explant collection and propagation material update table 12 Reference 13

Foreword

Appendix A, Appendix B, Appendix C and Appendix D of this standard are informative annexes. This standard was proposed by the Chinese Academy of Forestry. This standard is under the jurisdiction of the National Forest Seed Standardization Technical Committee. This standard is drafted by. Institute of Tropical Forestry, Chinese Academy of Forestry. Participated in the drafting of this standard. Wanjiang Forestry Biotechnology Research Institute, Zhanjiang, Guangdong Province. The main drafters of this standard. Zeng Bingshan, Xu Daping, Yan Zhenfei, Liu Ying, Li Xiangyang, Zhou Xiansheng. Technical regulation of rapid propagation of eucalyptus clones

1 Scope

This standard specifies the general principles for rapid propagation of seedlings in tropical and southern subtropical eucalyptus clones, including selection of parent trees and explants. Principles of collection and processing, medium preparation, aseptic processing, tissue culture seedling transplantation, seedling management and emergence, and disposal of waste media. This standard applies to the production of eucalyptus clonal seedlings in tissue culture plants, which are used for afforestation and establishment of saplings.

2 Normative references

The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard. GB 6000 Main afforestation tree seedling quality grading GB/T 6001 nursery technical regulations LY/T 1000 container seedling technology

3 Terms and definitions

The following terms and definitions apply to this standard. 3.1 Explants or culture materials release phenolic substances and oxidize to produce terpenoids. Browning has an explant or culture material Certain toxic effects. 3.2 The culture medium or the culture material grows with microorganisms such as fungi or bacteria. 3.3 For accurate and convenient weighing, the medium is dispensed into a solution or mixed solution of a certain concentration and ratio. 3.4 Illuminated with light. The usual plant tissue culture requires 12 hours to 16 hours of light per day. 3.5 All day without light culture. Some plants require dark culture when the explants are cultured or when callus is regenerated. 3.6 In vitro germination, callus or suspension culture cells continue to multiply the culture process. 3.7 A culture process for inducing roots from rootless in vitro shoots. ......

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