LST6114-2015 English PDF

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LST6114-2015: Inspection of grain and oils. Detection of ochratoxin A in grain. Rapid quantitative method of colloidal gold technique
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
LS/T 6114-2015	139	Add to Cart	3 days	Inspection of grain and oils. Detection of ochratoxin A in grain. Rapid quantitative method of colloidal gold technique	Valid

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GB/T 5519   LS/T 6117   SN/T 0800.18   LS/T 6115   LS/T 6111   

Basic data

Standard ID: LS/T 6114-2015 (LS/T6114-2015)
Description (Translated English): Inspection of grain and oils. Detection of ochratoxin A in grain. Rapid quantitative method of colloidal gold technique
Sector / Industry: Food & Beverage Industry Standard (Recommended)
Classification of Chinese Standard: B20
Classification of International Standard: 60.06
Word Count Estimation: 6,658
Date of Issue: 2015-07-10
Date of Implementation: 2015-07-10
Quoted Standard: GB 5491; GB/T 6682; GB/T 27404-2008
Regulation (derived from): State-Food-Communication [2015] No.3
Issuing agency(ies): National Grain Administration
Summary: This Standard specifies the ochratoxin A colloidal gold rapid quantitative detection method principle, reagents and materials, equipment, sample preparation, sample measurement and results are expressed. This Standard applies to wheat, corn, oats and other grains and products ochratoxin A rapid quantitative detection of toxins. The detection limit for the 3��g/kg.

LST6114-2015: Inspection of grain and oils. Detection of ochratoxin A in grain. Rapid quantitative method of colloidal gold technique

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Inspection of grain and oils.Detection of ochratoxin A in grain.Rapid quantitative method of colloidal gold technique ICS 60.060 B20 Record number. 50653-2015 People's Republic of China food industry standard Determination of ochratoxin A in grain and oil Colloidal gold rapid quantification Released on.2015-07-10 2015-07-10 implementation National Food Bureau released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009 and GB/T.20001.4-2001. This standard was proposed by the National Grain Administration. This standard is under the jurisdiction of the National Grain and Oil Standardization Technical Committee (SAC/TC270). This standard is drafted by. Anhui Provincial Grain and Oil Product Quality Supervision and Inspection Station. Participated in the drafting of this standard. Fujian Provincial Grain and Oil Quality Monitoring Institute, Henan Provincial Grain and Oilstuff Product Quality Supervision and Testing Center, Sichuan Grain Oil Center Monitoring Station, China Reserve Grain Management Corporation, Liaoning Provincial Grain and Oil Inspection and Monitoring Institute, Jiangsu Grain Bureau Grain and Oil Quality Monitoring Institute. The main drafters of this standard. Ji Yishun, Li Shuguang, Huang Jianli, Ji Jiansheng, Li Guiyou, Yuan Xiaoping, Zhu Xudong, Mo Xiaosong. Determination of ochratoxin A in grain and oil Colloidal gold rapid quantification

1 Scope

This standard specifies the principle, reagents and materials, equipment and samples of the rapid quantitative detection method of colloidal gold for ochratoxin A. Preparation, sample determination and results presentation. This standard applies to the rapid quantitative detection of ochratoxin A in wheat, corn, oats and other foods and their products. The method detection limit was 3 μg/kg.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB 5491 Inspection of food and oil materials, sample method GB/T 6682 Analytical laboratory water specifications and test methods GB/T 27404-2008 Laboratory quality control specification Food physical and chemical testing

3 Principle

Ochratoxin A in the sample extract reacts with colloidal gold particles in the test strip, color depth and ochratoxin in the sample The content of A is related. Use a reader to determine the color depth of the test line and the quality control line on the test strip, according to the color depth and the built-in curve of the reader The ochratoxin A content in the sample was automatically calculated.

4 reagents

Unless otherwise stated, the reagents used are of analytical grade, and laboratory water should meet the requirements of Class III water in GB/T 6682. 4.1 Dilution Buffer. Provided by a colloidal gold test strip or prepared according to its instructions. 4.2 Extract. 70% (volume fraction) methanol solution or colloidal gold test strip supporting extract.

5 instruments and materials

5.1 Balance. The division value is 0.01g. 5.2 Crusher. The sample can be pulverized and passed through a 20 mesh sieve. 5.3 Centrifuge. The speed is not less than 4000r/min. 5.4 vortex oscillator. 5.5 Incubator. It can be incubated at 45 °C (temperature control accuracy ± 1 °C) with time adjustment. 5.6 Reader. The measurement result of the colloidal gold quantitative test strip can be measured and displayed. 5.7 ROSA ochratoxin A colloidal gold rapid quantitative test strip 1). need to be stored in cold storage, the specific storage conditions refer to the instructions for use; ......

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