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JJF 1817-2020 English PDFUS$349.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. JJF 1817-2020: Calibration Specification for Nucleic Acids Analyzers Status: Valid
JJF 1817-2020: Calibration Specification for Nucleic Acids Analyzers---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Calibration Specification for Nucleic Acids Analyzers National Measurement Technical Specification of the People's Republic of China Calibration Specification for Nucleic Acid Analyzer 2020-01-17 release 2020-04-17 Implementation Issued by the State Administration of Market Supervision and Administration Calibration Specification for Nucleic Acid Analyzer Focal point. National Biometrics Technical Committee Main drafting unit. China Academy of Metrology Kaijie Enterprise Management (Shanghai) Co., Ltd. Participating in the drafting unit. Chinese Academy of Agricultural Sciences This specification entrusts the National Biometrics Technical Committee to interpret The main drafters of this specification. Dong Lianhua (Chinese Academy of Metrology) Gao Yunhua (Chinese Academy of Metrology) Cao Yan (Kajie Enterprise Management (Shanghai) Co., Ltd.) Participating drafters. Sui Zhiwei (Chinese Academy of Metrology) Li Liang (Chinese Academy of Agricultural Sciences) Wang Jing (Chinese Academy of Metrology) table of ContentsIntroduction (Ⅱ) 1 Scope (1) 2 References (1) 3 Terms and units of measurement (1) 4 Overview (1) 5 Metrological characteristics (1) 5.1 Indication error (1) 5.2 Repeatability (1) 5.3 Fragment concentration linearity (2) 6 Calibration conditions (2) 6.1 Environmental conditions (2) 6.2 Calibration reference materials and calibration equipment (2) 7 Calibration items and calibration methods (2) 7.1 Indication error (2) 7.2 Repeatability (3) 7.3 Concentration linearity (3) 8 Expression of calibration results (4) 9 Recalibration time interval (4) Appendix A. Preparation and use of calibration reagents and solutions (5) Appendix B. Reference Format for Original Calibration Records (6) Appendix C. Reference Format of Internal Page of Calibration Certificate (7) Appendix D Example of uncertainty assessment of concentration indication error (8)IntroductionThe preparation of this specification is based on the test of the main technical parameters of the nucleic acid analyzer, based on JJF 1071-2010 The provisions of "National Rules for the Preparation of Metrology Calibration", JJF 1059.1-2012 "Measurement Uncertainty Evaluation and Expression" Make it. This specification is the first release. Calibration Specification for Nucleic Acid Analyzer1 ScopeThis specification is applicable to nucleic acid analyzers that analyze the length and concentration of nucleic acid fragments based on the principle of gel electrophoresis Calibration, the length range of applicable nucleic acid fragments is (100 ~ 1000) bp, for nuclear The calibration of the acid analyzer can be performed with reference to this specification. This specification does not apply to plate electrophoresis and genetic analyzers calibration.2 Reference documentsThis specification refers to the following documents. JJF 1265-2010 Biometric Terms and Definitions For dated reference documents, only the dated version applies to this specification; for those without date references The latest version (including all amendments) is applicable to this specification.3 Terms and units of measurement3.1 Fragment length Represents the size of a nucleic acid fragment in units of nucleotides (nt) or base pairs (bp). 3.2 basepair (bp) basepair Paired bases between two strands in nucleic acids. Such as adenine-thymine (AT) pair, adenine-uracil (A- U), guanine-cytosine (GC) pair, guanine-uracil (GU) pair, etc. The number of base pairs characterizes DNA Or the length of double-stranded RNA. [JJF 1265-2010,4.41]4 OverviewThe nucleic acid analyzer (hereinafter referred to as the analyzer) is based on the principle of gel electrophoresis technology and converts double-stranded nucleic acids of different sizes. The fragments are separated.In the presence of fluorescent dyes, the fluorescent dyes are embedded in the double-stranded nucleic acid to emit fluorescence. Signal detection and analysis equipment can be qualitative and quantitative according to the electrophoresis time and fluorescence signal value of each nucleic acid fragment analysis. The analyzer is usually composed of a sampler, capillary/microchip, fluorescence detection optical components, microcircuit control components, Computer and application software, can be used for single and multiple PCR fragments, restriction enzyme digested DNA, etc. Analysis of fragment length and concentration.5 Metrological characteristics5.1 Indication error Fragment length indication error and fragment concentration indication error. 5.2 Repeatability ......Basic dataStandard ID: JJF 1817-2020 (JJF1817-2020)Description (Translated English): Calibration Specification for Nucleic Acids Analyzers Sector / Industry: Metrology & Measurement Industry Standard Classification of Chinese Standard: A50 Classification of International Standard: 17.020 Word Count Estimation: 15,194 Date of Issue: 2020 Date of Implementation: 2020-04-17 Issuing agency(ies): State Administration for Market Regulation IMAGE
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