HYT153-2013 English PDF

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HYT153-2013: Detection of mutagenecity in sea water and sediment sample by Salmonella typhimurium/mammals microsomal enzyme test(Ames test)
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
HY/T 153-2013	319	Add to Cart	3 days	Detection of mutagenecity in sea water and sediment sample by Salmonella typhimurium/mammals microsomal enzyme test(Ames test)	Valid

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Basic data

Standard ID: HY/T 153-2013 (HY/T153-2013)
Description (Translated English): Detection of mutagenecity in sea water and sediment sample by Salmonella typhimurium/mammals microsomal enzyme test(Ames test)
Sector / Industry: Marine Industry Standard (Recommended)
Classification of Chinese Standard: Z17
Classification of International Standard: 07.060; 13.060
Word Count Estimation: 12,189
Quoted Standard: GB 15193.4-2003; GB 17378.5;
Regulation (derived from): Industry standard filing Notice 2013 No. 8 (No. 164 overall)
Issuing agency(ies): State Oceanic Administration
Summary: This standard specifies: Determination of water and sediment mutagenicity required sample collection, processing, testing, analysis, calculation, etc. Technical requirements. This standard applies to: water and sediment mutagenicity determination.

HYT153-2013: Detection of mutagenecity in sea water and sediment sample by Salmonella typhimurium/mammals microsomal enzyme test(Ames test)

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection of mutagenecity in sea water and sediment sample by Salmonella typhimurium/mammals microsomal enzyme test(Ames test) ICS 07.060;13.060 Z17 People's Republic of China Marine Industry Standard Determination of mutagenicity in sea water and sediment Salmonella typhimurium/mammalian microsomal enzyme test Released on.2013-04-25 2013-05-01 Implementation Issued by the State Oceanic Administration

Table of contents

Preface Ⅲ 1 Scope 1 2 Normative references 1 3 Terms and definitions 1 4 Method principle 1 5 Equipment 1 6 Reagents and materials 2 7 Selection, identification and preservation of strains 2 8 Sample collection and storage 3 9 Analysis Step 4 10 Results judgment 5 11 Result calculation 5 Appendix A (Normative Appendix) Identification Standards for Strain Biological Characteristics and Standard Diagnostic Mutagen 6 Reference 8 Table A.1 Results of identification criteria for biological characteristics of strains 6 Table A.2 Standard mutagen determination results and expression method 6 Table A.3 Recommended standard mutagens 7

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed by the East China Sea Environmental Monitoring Center of the State Oceanic Administration. This standard is under the jurisdiction of the National Marine Standardization Technical Committee (SAC/TC283). Drafting organizations of this standard. East China Sea Environmental Monitoring Center of State Oceanic Administration, East China Sea Standard Metrology Center of State Oceanic Administration. The main drafters of this standard. Wang Jinhui, Liu Caicai, Xu Ren, Cheng Xiangsheng, Wu Yichuan, Wu Jianyong, Zhang Yong, Xiang Lingyun, Chen Dong. Determination of mutagenicity in sea water and sediment Salmonella typhimurium/mammalian microsomal enzyme test

1 Scope

This standard specifies the techniques for sample collection, processing, testing, analysis, calculation, etc. required for the determination of mutagenicity in seawater and sediments Claim. This standard applies to the determination of mutagenicity of seawater and sediments.

2 Normative references

The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this article Pieces. For undated references, the latest version (including all amendments) applies to this document. GB 15193.4-2003 Salmonella typhimurium/mammalian microsomal enzyme test GB 17378.5 Marine Monitoring Specification Part 5.Sediment Analysis

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Reversemutation Under the action of chemical mutants, the bacteria are transformed from auxotrophic type to wild type. 3.2 Salmonella typhimurium reverse mutation test Using a group of Salmonella typhimurium histidine-deficient test strains to determine chemicals that cause Salmonella base pair substitutions or frameshift mutations The test method of back-mutation of histidine-deficient type induced by cytoplasm to wild type.

4 Principle of the method

The mutant (histidine-deficient) strain of Salmonella typhimurium by artificial mutagenesis cannot grow on a histidine-free medium. The histidine medium can grow normally. However, if there are mutagens in the medium without histidine, the Salmonella mutant Can revert to wild-type (phenotype), so it can grow on histidine-free medium, so the test can be checked according to the number of colonies formed Whether the substance is mutagenic. Certain mutagens require metabolic activation to make the Salmonella mutants produce back mutations, metabolic activation systems The system can use polychlorinated biphenyl (PCB)-induced rat liver homogenate (S-9) to prepare the S-9 mixture. Use Salmonella typhimurium/mammals The microsomal enzyme test detects the mutagenicity of seawater or sediment.

5 Equipment

This test should include the following equipment. ......

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