HYT151-2013 English PDF

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HYT151-2013: Detection of hemolytic toxins in marine microalgae. Erythrocyte lysis assay
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
HY/T 151-2013	289	Add to Cart	3 days	Detection of hemolytic toxins in marine microalgae. Erythrocyte lysis assay	Valid

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Basic data

Standard ID: HY/T 151-2013 (HY/T151-2013)
Description (Translated English): Detection of hemolytic toxins in marine microalgae. Erythrocyte lysis assay
Sector / Industry: Marine Industry Standard (Recommended)
Classification of Chinese Standard: Z17
Classification of International Standard: 07.060; 13.060
Word Count Estimation: 11,134
Quoted Standard: GB 17378.3-2007; HY/T 069-2005;
Regulation (derived from): Industry standard filing Notice 2013 No. 8 (No. 164 overall)
Issuing agency(ies): State Oceanic Administration
Summary: This standard specifies: detection of hemolytic toxin activity in marine microalgae blood cell methods and technical requirements. This standard applies to: natural seawater marine microalgae cells hemolysin check side, also applies to laboratory culture

HYT151-2013: Detection of hemolytic toxins in marine microalgae. Erythrocyte lysis assay

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection of hemolytic toxins in marine microalgae.Erythrocyte lysis assay ICS 07.060;13.060 Z17 People's Republic of China Marine Industry Standard Detection of hemolysin in marine microalgae by blood cell method Released on.2013-04-25 2013-05-01 Implementation Issued by the State Oceanic Administration

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed by the South China Sea Environmental Monitoring Center of the State Oceanic Administration. This standard is under the jurisdiction of the National Marine Standardization Technical Committee (SAC/TC283). Drafting organizations of this standard. South China Sea Environmental Monitoring Center of State Oceanic Administration, Jinan University. The main drafters of this standard. Li Xiuqin, Yang Weidong, Huang Chuguang, Xu Zhibin, Gao Yang, Peng Yinghui, Shangguan Maosen, Lin Duan, Fang Hongda, Wu Jinxiao, Dong Yanhong, Chen Jiahui, Wu Lingling, Qu Niandong, Liu Jiesheng. Detection of hemolysin in marine microalgae by blood cell method

1 Scope

This standard specifies the method and technical requirements for the detection of hemolysin activity in marine microalgae by hematology. This standard applies to the detection of hemolysin in marine microalgae cells in natural seawater, and also applies to marine microalgae cells cultured in the laboratory Detection of hemolytic toxins.

2 Normative references

The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this article Pieces. For undated references, the latest version (including all amendments) applies to this document. GB 17378.3-2007 Marine Monitoring Specification Part 3.Sample Collection, Storage and Transportation HY/T 069-2005 red tide monitoring technical regulations

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Marine microalgae Phytoplankton with a particle size greater than 2μm in seawater. 3.2 Hemolytictoxins A class of glycolipid substances that are mainly produced by microalgae that can cause the lysis of red blood cells and other eukaryotic cells in the body. The lipid portion of the cell membrane of a cell. 3.3 Hemolytic activity The effectiveness of hemolysin to dissolve blood cells. 3.4 Hemolyticunits Used to express the dimension of hemolytic toxin activity. 1 hemolysis unit is equivalent to 1mL reaction system (containing citrate buffer 0.15mL, 0.5% rabbit red blood cells 0.80mL and hemolysin crude extract 0.05mL), lyse the red blood cells in a 37°C water bath for 30 minutes The amount of hemolytic toxin needed to dissolve 50% is equivalent to (1.30±0.39)μg digitalis saponins. 3.5 Erythrocytelysis assay A detection method established for the characteristics of hemolysin to destroy and dissolve blood cells. This method uses hemolysis unit as the dimension to quantify hemolysis Hemolytic activity of toxins. 3.6 Red tide water Seawater samples whose microalgae cell concentration meets or exceeds the reference concentration specified in HY/T 069-2005. ......

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