HYT147.5-2013 English PDF

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
HY/T 147.5-2013	RFQ	ASK	3 days	Code of practice for marine monitoring technology. Part 5: Marine ecology	Valid

Similar standards

GB/T 33673   DZ/T 0175   HY/T 147.2   HY/T 147.3   HY/T 147.1   

Basic data

Standard ID: HY/T 147.5-2013 (HY/T147.5-2013)
Description (Translated English): Code of practice for marine monitoring technology. Part 5: Marine ecology
Sector / Industry: Marine Industry Standard (Recommended)
Classification of Chinese Standard: A45
Classification of International Standard: 07.060
Word Count Estimation: 149,154
Quoted Standard: GB 3097; GB 4789.4-2010; GB 4789.30-2010; GB 4789.38; GB/T 12763.1; GB/T 12763.6-2007; GB 17378.3; GB 17378.5; GB 17378.7-2007; GB 18668; GB 19489; marine disaster survey specifications 3: marine Ecological disaster Investigation;
Regulation (derived from): Industry standard filing Notice 2013 No. 8 (No. 164 overall)
Issuing agency(ies): State Oceanic Administration
Summary: This standard specifies: marine life ecology sample collection, testing, analysis, data compilation methods technical requirements. This standard applies to: inshore, offshore, far from the sea waters biological and ecological monitored.

HYT147.5-2013: Code of practice for marine monitoring technology. Part 5: Marine ecology

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Code of practice for marine monitoring technology.Part 5.Marine ecology ICS 07.060 A45 People's Republic of China Marine Industry Standard Marine monitoring technical regulations Part 5.Marine Ecology Released on.2013-04-25 2013-05-01 Implementation Issued by the State Oceanic Administration

Table of contents

Preface Ⅴ 1 Scope 1 2 Normative references 1 3 Terms and definitions 1 4 Total number of planktonic viruses-dye counting method 2 5 The total number of viruses in the sediment-staining counting method 4 6 Total number of Vibrio ---plate counting method 6 7 Enterococcus---the most probable number method (MPN) and membrane filter method 6 8 Fecal coliforms---test piece method 6 9 Graded chlorophyll a---fluorescence method 11 10 Graded Primary Productivity---14C Isotope Method 15 11 Red tide dinoflagellate cysts---optical microscope method 18 12 Pico Phytoplankton---Fluorescence microscope counting method 18 13 Microplankton---microscopic individual counting method 20 14 Small plankton---microscopic individual counting method 24 15 Fish plankton---stereomicroscope counting method 24 16 Investigation of Rare and Endangered Animals---Interview and Observation Method 27 17 Coastal Wetland Plants---Field Investigation Method 30 18 Diarrheal shellfish poisoning---enzyme-linked immunosorbent assay (ELISA) 33 19 Paralytic shellfish poison---enzyme-linked immunosorbent assay (ELISA) 33 20 Paralytic shellfish poison-high performance liquid method (HPLC) 33 21 Amnestic shellfish poisoning---enzyme-linked immunosorbent assay (ELISA) 36 22 Neurogenic shellfish poison ---enzyme-linked immunosorbent assay (ELISA) 36 23 Staphylococcus aureus-test piece method 36 24 Salmonella-Microplate Kit Method 39 25 Listeria---test piece method 42 26 Fungus---test piece method 45 27 Vibrio parahaemolyticus in seawater---polymerase chain reaction (PCR) method 47 28 Vibrio vulnificus in seawater --- polymerase chain reaction (PCR) method 49 29 Vibrio fluvialis in seawater---polymerase chain reaction (PCR) method 50 30 Vibrio alginolyticus in seawater --- polymerase chain reaction (PCR) method 51 31 Vibrio harveyi in seawater --- polymerase chain reaction (PCR) method 52 32 Vibrio cholerae in seawater --- polymerase chain reaction (PCR) method 53 33 Vibrio anguillarum in seawater --- polymerase chain reaction (PCR) method 54 34 Hepatitis A virus in seawater --- reverse transcription polymerase chain reaction (RT-PCR) method 55 35 Norovirus in seawater --- reverse transcription polymerase chain reaction (RT-PCR) method 55 36 Astrovirus in seawater --- reverse transcription polymerase chain reaction (RT-PCR) method 55 37 Rotavirus in seawater---Reverse transcription polymerase chain reaction (RT-PCR) method 57 38 Adenovirus in seawater-polymerase chain reaction (PCR) method 58 39 Enterovirus in seawater --- reverse transcription polymerase chain reaction (RT-PCR) method 59 40 Shrimp Taura Virus---Reverse Transcription Polymerase Chain Reaction (RT-PCR) Method 60 41 Shrimp White Spot Syndrome Virus---polymerase chain reaction (PCR) method 60 42 Fish lymphatic cyst virus---polymerase chain reaction (PCR) method 60 43 Fish iridescent virus---polymerase chain reaction (PCR) method 61 44 Shellfish Perkinworm --- Reye's liquid thioglycollate medium culture (RFTM) method and polymerase chain reaction (PCR) method 61 45 Shellfish Monospora---polymerase chain reaction (PCR) method 61 46 Microbial molecular identification---restriction endonuclease fragment length polymorphism (RFLP) method 61 47 Microbial tracing monitoring of fecal contamination sources---repetitive extra-gene palindromic sequence polymerase chain reaction (REP-PCR) method 64 48 Molecular identification of red tide algae species---Fluorescence in situ hybridization (FISH) 68 49 Marine Pollutants Biological Toxicity Test---Luminescent Bacteria Method 70 50 Marine Pollutants Biological Toxicity Test---Algae Test Method 73 51 Marine Pollutants Biological Toxicity Test---Polychaete Test Method 76 52 Marine Pollutants Biological Toxicity Inspection---Mollusk Inspection Law 80 53 Marine Pollutants Biological Toxicity Inspection---Crustacean Inspection Law 82 54 Marine Pollutants Biological Toxicity Test---Echinoderma Test Method 85 55 Marine Pollutants Biological Toxicity Inspection---Fish Inspection Law 87 56 Marine Sediment Biological Toxicity Test---Amphipod Test Method 89 Appendix A (Normative Appendix) Record Form 96 Appendix B (informative appendix) Counting table of physiological saline, bacteria and fungi 129 Appendix C (Normative Appendix) Table of Most Probable Number (MPN) of Bacteria in Tested Samples 132 Appendix D (Normative Appendix) Plankton sample number, biomass measurement, counting 133 Appendix E (Normative Appendix) Colorimetric Chart 135 Appendix F (Normative appendix) Salmonella confirmation test flow chart 136 Appendix G (Normative Appendix) Listeria monocytogenes confirmation test procedure 137 Appendix H (informative appendix) PCR method to detect 7 kinds of Vibrio electrophoresis simulation diagram 138 Appendix I (informative appendix) PCR, RT-PCR method to detect 10 kinds of virus electrophoresis simulation diagram 139 Appendix J (informative appendix) Test organisms---Japanese big chela 140 Figure 1 Detection procedure of direct counting method for fecal coliform test piece 8 Figure 2 MPN counting method detection procedure of fecal coliform test piece 10 Figure 3 The age structure model of Amphioxus fig 30 Figure 4 Direct counting method test procedure for Staphylococcus aureus test piece 37 Figure 5 MPN counting method test procedure for Staphylococcus aureus test piece 39 Figure 6 Process of rapid detection of Salmonella by microplate kit method 40 Figure 7 Test procedure for Listeria monocytogenes test piece 44 Figure 8 Inspection procedures for mold and yeast 46 Figure E.1 Interpretation of the results of the Salmonella microplate kit detection color chart 135 Figure F.1 Salmonella confirmation test flow chart 136 Figure G.1 Listeria monocytogenes confirmation test process 137 Figure H.1 PCR method to detect 7 kinds of Vibrio electrophoresis simulation diagram 138 Figure I.1 The electrophoresis simulation diagram of 10 viruses detected by PCR and RT-PCR methods 139 Table 1 Water sample collection level 11 Table 2 Specifications and applicable objects of various nets 25 Table 3 Plant community sample plot description survey form 31 Table 4 Reference Table 35 for the detection limits of various paralytic shellfish poisons Table 5 Probes used in fluorescence in situ hybridization test 69 Table 6 The dilution factor of mercury chloride working solution 71 Table A.1 Plankton viruses and picoplankton marine sampling records 96 Table A.2 The direct count record of planktonic or sediment viruses 97 Table A.3 Chlorophyll sampling record table 98 Table A.4 Chlorophyll (Extraction Fluorescence Method) Measurement Record Table 99 Table A.5 Primary Productivity Granularity Classification Sampling, Filtration, and Measurement Record Table 100 Table A.6 Record of the number of picophytoplankton cells 101 Table A.7 Marine Sampling Record of Microplankton 102 Table A.8 Microplankton sample registration form 103 Table A.9 Water sample plankton number count record table 104 Table A.10 Number of micro-plankton specimens counted records 105 Table A.11 Statistics on the number of microplankton 106 Table A.12 List of microplankton species 107 Table A.13 Marine Collection Record of Fish Plankton 108 Table A.14 Fish plankton specimen registration form 109 Table A.15 Fish plankton count record table 110 Table A.16 Statistics of fish plankton quantity 111 Table A.17 Marine Rare and Endangered Swimming Animal Observation Record Table 112 Table A.18 Statistics of observation results of marine rare and endangered swimming animals 112 Table A.19 Observation records of rare and endangered sea birds sample points Table 113 Table A.20 Observation Records of Rare and Endangered Sea Bird Transects Table 113 Table A.21 Wenchang fish field sampling record 114 Table A.22 Indoor analysis record of Amphioxus population structure 114 Table A.23 Investigation record of coastal wetland plants 115 Table A.24 Test record of pathogenic Vibrio in seawater 116 Table A.25 Marine Virus Record Form 117 Table A.26 Fish lymphatic cyst virus detection record table 118 Table A.27 Biological toxicity test site sampling record table 119 Table A.28 Luminescent bacteria acute toxicity test record table 120 Table A.29 Luminescent bacteria method to determine environmental toxicity classification standards 120 Table A.30 Algae toxicity test record table 121 Table A.31 Percentage and probability unit conversion table 122 Table A.32 Algae toxicity test data statistical conversion registration form 123 Table A.33 Marine biological toxicity test record table 124 Table A.34 Marine biological toxicity test data statistical conversion registration form 125 Table A.35 Amphipod toxicity test biological result record table 126 Table A.36 Amphipod toxicity test physical result record table 127 Table A.37 Amphipod toxicity test biological results statistics table 128 Table B.1 Direct count of bacteria (fungus) test piece 130 Table B.2 Bacteria test piece MPN count table 131 Table C.1 The most probable number (MPN) of bacteria in the tested sample Table 132

Foreword

HY/T 147 "Marine Monitoring Technical Regulations" is divided into seven parts. ---Part 1.Sea water; ---Part 2.Sediment; ---Part 3.Organisms; ---Part 4.Ocean atmosphere; ---Part 5.Marine ecology; ---Part 6.Marine Hydrology, Meteorology and Sea Ice; ---Part 7.Satellite remote sensing technology methods. This part is part 5 of HY/T 147. This section was drafted in accordance with the rules given in GB/T 1.1-2009. This part was proposed by the National Marine Environmental Monitoring Center. This part is under the jurisdiction of the National Marine Standardization Technical Committee (SAC/TC283). Drafting organizations of this section. National Marine Environmental Monitoring Center, South China Sea Environmental Monitoring Center of State Oceanic Administration, East China Sea Environmental Monitoring Center of State Oceanic Administration Monitoring Center, North Sea Environmental Monitoring Center of State Oceanic Administration. The main drafters of this section. Han Gengchen, Fan Jingfeng, Ma Yongan, Jiang Wenbo, Zhang Zhendong, Lin Fengao, Li Hongbo, Liang Bin, Xu Daoyan, Shao Kuishuang, Li Dongmei, Liu Yongjian, Liu Shuxi, Yuan Xiutang, Yan Qilun, Liu Guize, Wang Lijun, Liu Changan, Feng Zhiquan, Wang Zhenliang, Liu Na, Yu Zhanguo, Huang Chuguang, Gao Yang, Li Xiuqin, Wei Guiqiu, Yi Bin, Xiao Yuzhang, Chen Jiahui, Li Haitao, Xiong Xiaofei, Dong Yanhong, Wu Shiwei, Lu Chuqian, Cheng Xiangsheng, Liu Caicai, Sun Yawei, Qin Yutao, Li Yiyun, Xia Yongjian, Fan Lijing, Ji Huanhong, Huang Hui, Gao Junzhang, Xin Dinghao, Song Chenyao, Cui Wenlin, Qi Yanping, Zhang Qi, Wang Taisen, Wang Xiaoqing, Li Qinliang, Zhao Sheng, Sun Beibei, Zhang Qingbo. Marine monitoring technical regulations Part 5.Marine Ecology

1 Scope

This part of HY/T 147 specifies the technical requirements for the methods of sampling, testing, analysis, and data compilation of marine biological ecology. This part is applicable to the monitoring of biological ecology in nearshore, offshore and far sea areas.

2 Normative references

The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this article Pieces. For undated references, the latest version (including all amendments) applies to this document. GB 3097 Sea Water Quality Standard GB 4789.4-2010 National Food Safety Standard Food Microbiological Inspection Salmonella Inspection GB 4789.30-2010 National Food Safety Standard Food Microbiological Examination Examination of Listeria Monocytogenes GB 4789.38 National Food Safety Standard, Food Microbiological Inspection, Count of Escherichia Coli GB/T 12763.1 Marine Survey Specification Part 1.General GB/T 12763.6-2007 Marine Survey Specification Part 6.Marine Biological Survey GB 17378.3 Marine Monitoring Specification Part 3.Sample Collection, Storage and Transportation GB 17378.5 Marine Monitoring Specification Part 5.Sediment Analysis GB 17378.7-2007 Marine Monitoring Specification Part 7.Offshore Pollution Ecological Investigation and Biological Monitoring GB 18668 Marine sediment quality GB 19489 Laboratory Biosafety General Requirements Technical Specifications for Marine Disaster Investigation Part 3.Marine Ecological Disaster Investigation

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Plankton plankton A creature that lacks developed motor organs, has no or only weak motor ability, is suspended in the water layer, and often moves with water. Including float There are two major categories of zooplankton and zooplankton. Plankton can be divided into the following types according to the size of the individual. those with a particle size of less than 2μm are called pico Plankton (picoplankton); the particle size of 2μm~20μm is called nanoplankton; the particle size is 20μm~ 200μm is called small plankton (microplankton or netplankton);.200μm~2000μm is called medium plankton Organisms (mesoplankton); those with a particle size between.2000μm and 20mm are called macroplanktons; the particle size is greater than 20mm is called megaplankton. In addition, fish plankton (ichthyoplankton) are fish eggs and larvae. [GB/T 12763.6-2007, definition 3.10] 3.2 Distance sampling distancesampling Using bird identification technology at different distances and habitats, estimate the number of birds within a certain range or distance, and collect ecological population data. ......

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