GBZT316.3-2018 English PDFUS$119.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GBZT316.3-2018: Determination of lead in blood -- Part 3: Atomic fluorescence spectrometry method Status: Valid
Basic dataStandard ID: GBZ/T 316.3-2018 (GBZ/T316.3-2018)Description (Translated English): Determination of lead in blood -- Part 3: Atomic fluorescence spectrometry method Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: C60 Word Count Estimation: 6,676 Date of Issue: 2018-08-16 Date of Implementation: 2019-01-01 Regulation (derived from): State-Health-Communication (2018) No.14 Issuing agency(ies): National Health and Family Planning Commission GBZ/T 316.3-2018: Determination of lead in blood -- Part 3: Atomic fluorescence spectrometry method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Determination of lead in blood - Part 3. Atomic fluorescence spectrometry method ICS 13.100 C 52 National Occupational Health Standards Published on.2018 - 08 - 16 2019 - 01 - 01 implementation Determination of lead in blood Part 3. Atomic Fluorescence Spectroscopy Determination of lead in blood- Part 3. Atomic fluorescence spectrometry method National Health and Wellness Committee of the People's Republic of China ForewordThis standard is formulated in accordance with the Law of the People's Republic GBZ /T 316-2018 "Determination of lead in blood" is divided into three parts. -- Part 1. Graphite furnace atomic absorption spectrometry; -- Part 2. Inductively coupled plasma mass spectrometry; -- Part 3. Atomic Fluorescence Spectrometry. This part is the third part of GBZ /T 316. This part is drafted in accordance with the rules given in GB/T 1.1-2009. This section drafted by. Beijing Municipal Center for Disease Control and Prevention, China Center for Disease Control and Prevention, Occupational Health and Poison Control Institute, Zhejiang Provincial Academy of Medical Sciences, Kaifeng City Center for Disease Control and Prevention, Henan Province. The main drafters of this section. Chen Heng, Pan Yajuan, Zhang Jing, Tang Hongfang, Li Kun. Determination of lead in blood - Part 3. Atomic fluorescence spectroscopy1 ScopeThis part of GBZ /T 316 specifies atomic fluorescence spectrometry for the determination of lead in blood. This section applies to the determination of lead in the blood of occupational contacts.2 Normative referencesThe following documents are indispensable for the application of this document. For dated references, only the dated version applies to this document. For undated references, the latest edition (including all amendments) applies to this document. General rules for biological monitoring of GBZ /T 295 occupational population3 PrincipleThe blood sample (hereinafter referred to as the blood sample) is deproteinized with nitric acid, and the supernatant is taken after centrifugation, and the hydride is reduced to a wavelength of 283.3 nm. Next, the lead content was measured by an atomic fluorescence spectrometer.4 instruments4.1 Volumetric flask, 10mL. 4.2 stoppered polyethylene centrifuge tube, 5mL. 4.3 High-speed centrifuge, the speed is greater than 10000r/min. 4.4 Vortex mixer. 4.5 Micropipettes, the range is 20μL ~.200μL, 0.5mL ~ 5.0 mL. 4.6 Atomic Fluorescence Spectrometer with high performance lead hollow cathode lamp and hydride generation device.5 reagent5.1 Deionized water. 5.2 Nitric acid, ρ20=1.42g/mL, excellent grade pure. 5.3 Nitric acid solution (for sample processing and current carrying), 3.5% (V/V). Dilute 3.5 mL of nitric acid to 100 mL with water. 5.4 sodium hydroxide solution, 30g/L. 5.5 Reducing agent solution. Weigh 10g potassium borohydride (excellent grade pure), dissolve in sodium hydroxide solution, add 20g potassium ferricyanide (analytical grade) And 10 g of boric acid (premium pure), then dissolved in sodium hydroxide solution and diluted to 1000 mL. 5.6 Lead single element standard solution. 6 Sample collection, transportation and storage According to GBZ /T 295. The collected samples and sample blanks are placed in a clean container for refrigerated transport. The sample can be stored for half a year at -20 °C.7 Analysis steps7.1 Instrument Operation Reference Conditions See Table 1. Table 1 Instrument operation reference conditions Wavelength/nm 283.3 lamp current/mA 60 Atomizer temperature/°C.200 cathode auxiliary lamp current/mA 25 Photomultiplier tube negative high voltage/V 270 delay time/s 1.0 Atomizer height/mm 8 reading time/s 8 Carrier gas (Ar) flow rate/(mL/min) 400 injection volume/mL 0.8 Shielding gas (Ar) flow rate/(mL/min) 800 reading method peak area 7.2 Preparation of the standard series Dilute lead standard solution with nitric acid solution to prepare 0.0μg/L, 2.0μg/L, 5.0μg/L, 10.0μg/L, 15.0μg/L, 20.0μg/L, 30.0μg/L The lead standard series are installed in 7 volumetric flasks, numbered 1~7. 7.3 Processing of samples and sample blanks The frozen blood sample was taken out and returned to room temperature. Shake well, take 0.10 mL, and place it in a centrifuge tube containing 2.90 mL of nitric acid solution. In the middle, immediately cover the lid, shake vigorously, then mix well in the vortex mixer for 5 min, centrifuge at 10000 r/min for 10 min, take the supernatant The liquid is measured. Blank processing of the sample. 2.0 mL of water was taken with a blood collection needle and placed in a blood collection tube, and shaken, and the remaining treatment steps were the same as the sample. 7.4 Determination of standard series, samples and sample blanks Refer to the operating conditions of the instrument, adjust the instrument to the best measurement state, the injection volume is 0.8mL, respectively determine the standard series, each concentrated Repeat the measurement 3 times, the average fluorescence intensity of the 2nd to 7th minus the fluorescence intensity of the 1st, and the fluorescence intensity average to the lead concentration (μg/L) Draw a standard curve or calculate a regression equation. The sample solution and the sample blank solution are determined by measuring the operating conditions of the standard series, and the measured fluorescence intensity value is determined by a standard curve or a regression curve. The concentration of lead (μg/L) was calculated.8 calculationCalculate the concentration of lead in blood according to formula (1). C = C0×F (1) In the middle C - the concentration of lead in the blood, μg/L; C0--lead concentration from standard curve or regression equation (minus sample blank), μg/L; F -- sample dilution factor.9 Description9.1 The detection limit of this method is 6.7μg/L, and the lower limit of quantification is 20μg/L (according to 0.1mL blood sample, 30 times dilution); the measurement range is 20μg/L ~ 900μg/L; relative standard deviation range of 2.72% ~ 7.01% (n = 6); blood sample spike recovery range of 95.3% ~ 106.0%. 9.2 Adding potassium ferricyanide to the reducing agent can promote the formation of lead hydride; the prepared reducing agent can be stably stored at 4 ° C for 1 week. 9.3 The concentration of nitric acid solution and reducing agent has a great influence on the hydrogenation reaction, and the same batch of samples should be strictly consistent. 9.4 If the lead concentration in the sample exceeds the measurement range, it can be determined by diluting with a nitric acid solution, and multiplying by the corresponding dilution factor. 9.5 This method should strictly control the injection volume, and the injection volume will generate a large number of bubbles during the hydride reaction, which will affect the normal measurement. 9.6 EDTA anticoagulant should not be used for blood collection. 9.7 Quality control of the testing process shall be carried out in accordance with the requirements of GBZ /T 295. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GBZT316.3-2018_English be delivered?Answer: Upon your order, we will start to translate GBZT316.3-2018_English as soon as possible, and keep you informed of the progress. 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