GBZT316.1-2018 English PDF

US$159.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GBZT316.1-2018: Determination of lead in blood -- Part 1: Graphite furnace atomic absorption spectrometry method
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GBZ/T 316.1-2018	159	Add to Cart	3 days	Determination of lead in blood -- Part 1: Graphite furnace atomic absorption spectrometry method	Valid

Similar standards

GBZ 20   GBZ 57   GBZ 49   GBZ/T 330   GBZ/T 329   GBZ/T 316.2   

Basic data

Standard ID: GBZ/T 316.1-2018 (GBZ/T316.1-2018)
Description (Translated English): Determination of lead in blood -- Part 1: Graphite furnace atomic absorption spectrometry method
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: C60
Word Count Estimation: 8,899
Date of Issue: 2018-08-16
Date of Implementation: 2019-01-01
Older Standard (superseded by this standard): WS/T 20-1996
Regulation (derived from): State-Health-Communication (2018) No.14
Issuing agency(ies): National Health and Family Planning Commission

GBZ/T 316.1-2018: Determination of lead in blood -- Part 1: Graphite furnace atomic absorption spectrometry method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of lead in blood - Part 1. Graphite furnace atomic absorption spectrometry method ICS 13.100 C 52 National Occupational Health Standards Replace WS/T 20-1996 Published on.2018 - 08 - 16 2019 - 01 - 01 implementation Determination of lead in blood Part 1. Graphite furnace atomic absorption spectrometry Determination of lead in blood- Part 1. Graphite furnace atomic absorption spectrometry method National Health and Wellness Committee of the People's Republic of China

Foreword

This standard is formulated in accordance with the Law of the People's Republic GBZ /T 316-2018 "Determination of lead in blood" is divided into three parts. -- Part 1. Graphite furnace atomic absorption spectrometry; -- Part 2. Inductively coupled plasma mass spectrometry; -- Part 3. Atomic Fluorescence Spectrometry. This part is the first part of GBZ /T 316. This part is drafted in accordance with the rules given in GB/T 1.1-2009. This section replaces WS/T 20-1996 "Graphite Furnace Atomic Absorption Spectrometry for Lead in Blood". Compared with WS/T 20-1996, the main changes are as follows. -- The operating conditions of the instrument are modified to the reference conditions for instrument operation; -- Improved standard formulation and sample handling methods; -- The background correction system of the instrument was clarified. This section drafted by. China Center for Disease Control and Prevention, Occupational Health and Poison Control. The main drafters of this standard. Pan Yajuan, Zhang Jing, Tao Xue, Yan Huifang. The previous versions of the standards replaced by this standard. --WS/T 20-1996. Determination of lead in blood -- Part 1 . Graphite furnace atomic absorption spectrometric method

1 Scope

This part of GBZ /T 316 specifies graphite furnace atomic absorption spectrometry for the determination of lead in blood. This section applies to the determination of lead in the blood of occupational contacts.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this document. For undated references, the latest edition (including all amendments) applies to this document. General rules for biological monitoring of GBZ /T 295 occupational population 3 acid deproteinization-graphite furnace atomic absorption spectrometry 3.1 Principle Blood samples (hereinafter referred to as blood samples) are deproteinized with a nitric acid solution and measured by graphite furnace atomic absorption spectrometry at a wavelength of 283.3 nm. Determine the lead content. 3.2 Instrument 3.2.1 Volumetric flask, 10 mL. 3.2.2 stoppered polyethylene centrifuge tube, 1.5mL. 3.2.3 Vortex mixer. 3.2.4 Centrifuge, the speed is greater than 10000r/min. 3.2.5 Micropipettes, the range is 20μL~200μL, 100μL~1000μL. 3.2.6 Atomic absorption spectrometer with graphite furnace, Zeeman or xenon lamp background correction device and lead hollow cathode lamp. 3.3 Reagents 3.3.1 Deionized water. 3.3.2 Nitric acid. ρ20=1.42g/mL, excellent grade pure. 3.3.3 Nitric acid solution. 1% (volume fraction). 3.3.4 Nitric acid solution. 5% (volume fraction). 3.3.5 Bovine blood. Heparin anticoagulation, stored at -20 °C, shake it at room temperature. Low background human blood, sheep blood, etc. can also be used. Background lead content Should be less than 50μg/L. 3.3.6 Standard solution, using lead single element certified standard substance. 3.4 Sample collection, transportation and storage According to GBZ /T 295. The collected samples and sample blanks are placed in a clean container for refrigerated transport. The sample can be stored for half a year at -20 °C. 3.5 Analysis steps 3.5.1 Instrument Operation Reference Conditions Dry 80 ° C ~ 150 ° C 55s Ashing 300 ° C ~ 350 ° C 20s Atomization 1600 ° C 5s stop gas Clear 2400 ° C 3s 3.5.2 Preparation of blood lead standard working curve series The lead single element standard solution is diluted with a 1% nitric acid solution into a 50.0 μg/mL lead standard application solution, and then a 1% nitric acid solution is used to prepare a concentration of 0 μg/mL, 1.25 μg/mL, 2.50 μg/mL, 5.00 μg/mL, 10.00 μg/mL, and 12.50 μg/mL lead standard solution series. Take another 6 10mL Volumetric flasks, numbered from 1 to 6. Add 0.40mL of lead standard solution series with concentration of 0μg/mL~12.50μg/mL, respectively, and then use the bovine blood to make up the volume. To the scale, the blood lead working curve is prepared at concentrations of 0μg/L, 50μg/L, 100μg/L,.200μg/L, 400μg/L, and 500μg/L. Quasi-solution series. The preparation method is shown in Table 1. Table 1 Blood lead working curve standard series configuration Volumetric flask number 1 2 3 4 5 6 Lead standard solution series/(μg/mL) 0 1.25 2.50 5.00 10.00 12.50 Lead standard solution series/mL 0.40 0.40 0.40 0.40 0.40 0.40 Take bovine blood/mL 9.60 9.60 9.60 9.60 9.60 9.60 Lead standard solution in blood/(μg/L) 0 50 100.200 400 500 3.5.3 Blood lead standard working curve solution series, sample and sample blank pretreatment method 3.5.3.1 Blood lead standard working curve solution series pretreatment method. take 0.15mL blood lead standard solution working curve series respectively In an ethylene centrifuge tube, add 0.60 mL of 5% nitric acid solution to each tube, cover immediately, shake vigorously, and then shake on a vortex mixer. 5 min, centrifuged at 10,000 r/min for 5 min, and the supernatant was used for measurement. 3.5.3.2 Sample Pretreatment Method. Remove the frozen blood sample and return to the laboratory temperature. Shake well and mix well, remove 0.15mL, place In a 1.5 mL stoppered polyethylene centrifuge tube, the remaining treatment steps are the same as above. 3.5.3.3 Sample blank pretreatment method. 2.0 mL water was taken with a blood collection needle and placed in a blood collection tube, and shaken. The remaining treatment steps were the same as above. 3.5.4 Determination of blood lead standard working curve solution series, sample and sample blank 3.5.4.1 Determination of blood lead standard working curve solution series. adjust the atomic absorption spectrometer to the best according to the instrument operation reference conditions. For the measurement state, 15 μL of the supernatant was injected, and each standard series was measured, and each concentration was repeatedly measured three times. 2 to 6 absorbance value minus 1 After the absorbance value of the number, draw a working curve or calculate a regression equation for the corresponding lead concentration (μg/L). 3.5.4.2 Determination of sample and sample blanks. Determine the sample and sample blank solution using the operating conditions of the standard series of assays. The result should be less than the detection limit. When the test result is greater than the detection limit, it indicates that the sample is contaminated during the collection, transportation and storage process. Should be void. The measured absorbance value is calculated from the working curve or the regression equation for the concentration of lead (μg/L). 3.6 Calculation Calculate the concentration of lead in the blood sample according to formula (1). C = C0 (1) In the formula. C--the concentration of lead in blood, in micrograms per liter (μg/L); C0--The concentration of lead in a blood sample obtained from the working curve or regression equation in micrograms per liter (μg/L). 3.7 Description 3.7.1 The detection limit of this method is 7μg/L, the lower limit of quantification is 20μg/L; the measurement range is 20μg/L~500μg/L. Zeeman background corrected The relative standard deviation of the atomic absorption spectrometer ranges from 1.6% to 2.8% (n=6), and the background calibration of the atomic absorption spectrometer with xenon lamp is relatively standard deviation. The difference range is 1.5% to 5.3% (n=6). 3.7.2 The injection volume of this method should be determined according to the specific conditions of the instrument, generally choose 10μL ~ 20μL. 3.7.3 In this method, the matrix has an influence on the measurement. The sample should be treated in the same way as the working curve series solution. If the lead concentration in the sample Exceeding the measurement range, the blood lead working curve range can be increased to 800μg/L or 1000μg/L, and the standard series and samples are diluted by 10 times. After the method was measured, 0.1 mL of blood was taken, and 5% nitric acid solution was added to 1.0 mL. 3.7.4 The EDTA anticoagulation tube cannot be used for blood collection tubes. 3.7.5 Quality control of the testing process shall be carried out in accordance with the requirements of GBZ /T 295. 4 Triton dilution - graphite furnace atomic absorption spectrometry 4.1 Principle Blood samples (hereinafter referred to as blood samples) are diluted with a mixture of Triton and Nitric Acid and then absorbed by graphite furnace atom at a wavelength of 283.3 nm. Spectrometric determination of the concentration of lead. 4.2 Instrument 4.2.1 Volumetric flask. 5mL. 4.2.2 Plugged polyethylene centrifuge tube. 1.5mL. 4.2.3 Micropipette. The range is 20μL~200μL, 100μL~1000μL. 4.2.4 Atomic Absorption Spectrometer. with graphite furnace, Zeeman background correction device and lead hollow cathode lamp. 4.3 Reagents 4.3.1 The experimental water is deionized water. 4.3.2 Nitric acid. ρ20=1.42g/mL, excellent grade pure. 4.3.3 Nitric acid solution. 1% (volume fraction). 4.3.4 Triton X-100. Analytically pure. 4.3.5 Triton X-100 solution. 1% (volume fraction). 4.3.6 Diluent. Mix 20 mL of nitric acid solution (1%), 10 mL of Triton X-100 solution (1%) with 70 mL of water. 4.3.7 Bovine blood. Heparin anticoagulation, stored at -20 ° C, shake it at room temperature. Low background human blood, sheep blood, etc. can also be used. Background lead The amount should be less than 50 μg/L. 4.3.8 Standard solution, using lead single element standard solution. 4.4 Sample collection, transportation and storage According to GBZ /T 295. The collected samples and sample blanks are placed in a clean container for refrigerated transport. The sample can be stored for half a year at -20 °C. 4.5 Analysis steps 4.5.1 Instrument Operation Reference Conditions Dry 80~150°C, 10s Ashing 500~600°C, 30s, keep 10s Atomization 1800 ° C, 5 s, gas Purification 2400 ° C, 3 s 4.5.2 Preparation of blood lead standard working curve series The lead single element standard solution is diluted with a 1% nitric acid solution to a standard application solution of 50.0 μg/mL, and then a concentration of 1% nitric acid solution is used. 0 μg/mL, 1.25 μg/mL, 2.50 μg/mL, 5.00 μg/mL, 10.00 μg/mL, 20.00 μg/mL, 25.00 μg/mL standard solution series. Take 7 5mL volumetric flasks, numbered 1~7. Add 0.20mL of lead standard solution series with concentration of 0μg/mL~25.00μg/mL, respectively. The volume is set to 0μg/L, 50μg/L, 100μg/L,.200μg/L, 400μg/L, 800μg/L, 1000μg/L. The blood lead standard working curve solution series. See Table 2 for details. Table 2 blood lead standard working curve series preparation Volume bottle number 1 2 3 4 5 6 7 Lead standard solution series/(μg/mL) 0 1.25 2.50 5.00 10.00 20.00 25.00 Lead standard solution series/mL 0.20 0.20 0.20 0.20 0.20 0.20 0.20 Take bovine blood/mL 4.80 4.80 4.80 4.80 4.80 4.80 4.80 Blood lead standard solution series/(μg/L) 0 50 100.200 400 800 1000 4.5.3 Treatment of blood lead standard working curve solution series, sample and sample blank 4.5.3.1 Blood lead standard working curve solution series treatment. blood lead standard working curve series respectively take 0.10mL in the stoppered polyethylene Add 0.90 mL of diluent to each of the hearts, and shake well to mix. 4.5.3.2 Treatment of the sample. Remove the frozen blood sample and return to the laboratory temperature. Shake well and mix well, take 0.10mL and place in 1.5mL In a stoppered polyethylene centrifuge tube, add 0.90 mL of diluent and mix well by shaking. 4.5.3.3 Sample blank treatment. 2.0 mL water was taken from the blood collection tube and placed in the blood collection tube. The remaining treatment steps were the same as above. 4.5.4 Determination of blood lead standard working curve solution series, sample and sample blank 4.5.4.1 Determination of blood lead standard working curve solution series. adjust the atomic absorption spectrometer to the best according to the instrument operation reference conditions. For the measurement state, 15 μL of the supernatant was injected, and each standard series was measured, and each concentration was repeatedly measured three times. 2 to 7 absorbance value minus 1 After the absorbance value of the number, draw a working curve or calculate a regression equation for the corresponding lead concentration (μg/L). 4.5.4.2 Determination of sample and sample blanks. Determination of samples and blank sample solutions using the operating conditions of the standard series of assays, blank determination of knots The result should be less than the detection limit. When the test result is greater than the detection limit, it indicates that the sample is contaminated during the collection, transportation and storage process. Should be void. 4.6 Calculation Calculate the concentration of lead in the blood sample according to formula (2). C = C0 (2) In the formula. C--the concentration of lead in blood, in micrograms per liter (μg/L); C0--The lead concentration of the blood sample obtained from the standard curve or the regression equation in micrograms per liter (μg/L). 4.7 Description 4.7.1 Atomic absorption spectrometers with background correction of xenon lamps are not suitable for the determination of blood lead in this method. 4.7.2 The detection limit of this method is 7μg/L, the lower limit of quantification is 20μg/L; the measurement range is from 20μg/L to 1000μg/L. The relative standard deviation The range was 1.8%~5.1% (n=6); the recovery rate of blood samples was 99.9%~108.9% (spiking concentration was 100μg/L~800μg/L). 4.7.3 The matrix in this method has an influence on the determination. The sample and the working curve series should be treated in the same way. 4.7.4 The injection volume of this method should be determined according to the specific conditions of the instrument, generally choose 10μL~20μL. 4.7.5 The EDTA anticoagulation tube cannot be used in the blood collection tube. 4.7.6 Quality control of the testing process shall be carried out in accordance with the requirements of GBZ /T 295. ......

Image     

Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GBZT316.1-2018_English be delivered?

Answer: Upon your order, we will start to translate GBZT316.1-2018_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GBZT316.1-2018_English with my colleagues?

Answer: Yes. The purchased PDF of GBZT316.1-2018_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.