GBZT304-2018 English PDF

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GBZT304-2018: Determination of aluminum in urine -- Graphic furnace atomic absorption spectrometry method
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GBZ/T 304-2018	139	Add to Cart	3 days	Determination of aluminum in urine -- Graphic furnace atomic absorption spectrometry method	Valid

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Basic data

Standard ID: GBZ/T 304-2018 (GBZ/T304-2018)
Description (Translated English): Determination of aluminum in urine -- Graphic furnace atomic absorption spectrometry method
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: C60
Word Count Estimation: 7,722
Date of Issue: 2018-08-16
Date of Implementation: 2019-01-01
Regulation (derived from): State-Health-Communication (2018) No.14
Issuing agency(ies): National Health and Family Planning Commission

GBZ/T 304-2018: Determination of aluminum in urine -- Graphic furnace atomic absorption spectrometry method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of aluminum in urine - Graphic furnace atomic absorption spectrometry method ICS 13.100 C 52 National Occupational Health Standards Determination of aluminum in urine - Graphite furnace atomic absorption spectrometry Determination of aluminum in urine- Graphic furnace atomic absorption spectrometry method Published on.2018 - 08 - 16 2019 - 01 - 01 implementation National Health and Wellness Committee of the People's Republic of China

Foreword

This standard is formulated in accordance with the Law of the People's Republic This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is mainly drafted by. Guangdong Provincial Occupational Disease Prevention and Treatment Institute, Guangzhou Occupational Disease Prevention and Treatment Institute, Shenzhen Occupational Disease Prevention and Treatment Institute, Qingyuan City Center for Disease Control and Prevention, Guangdong Pharmaceutical University. The main drafters of this standard. Dong Ming, Zhang Aihua, Wu Banghua, Yan Xiaolin, Wang Jun, Li Tianyu, Lin Yiran, Yang Xueji, He Jianfei, Wu Shihua, Lai Wei, Cui Fan, Yang Zhanhong, Pan Qiaoyu, Sun Yi, Huang Limei. Determination of aluminum in urine - Graphite furnace atomic absorption spectrometry

1 Scope

This standard specifies graphite furnace atomic absorption spectrometry for the determination of aluminum in urine. This standard applies to the determination of aluminum in the urine of occupational contact personnel.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this document. For undated references, the latest edition (including all amendments) applies to this document. General rules for biological monitoring of GBZ /T 295 occupational population

3 Principle

Urine sample (hereinafter referred to as urine sample) plus diluent treatment, direct injection, at 339.3nm wavelength, using graphite furnace atomic absorption spectrometry The method measures the concentration of aluminum.

4 instruments

4.1 Graphite furnace atomic absorption spectrometer with background correction device and aluminum hollow cathode lamp. 4.2 Thermostatic platform pyrolytic graphite tube or pyrolytic coated graphite tube. 4.3 stoppered polyethylene plastic bottle, 100mL. 4.4 stoppered polyethylene plastic tube, 5mL, 2mL. 4.5 urine hydrometer. 4.6 Micropipettes, 1000L,.200L, 50L. 4.7 Glass instruments and plastic utensils are soaked in 50% nitric acid for more than 24 hours, rinsed, and dried for use.

5 reagent

5.1 Experimental water. Distilled water in deionized water or full glass distiller. 5.2 Nitric acid, ρ20=1.42 g/mL, excellent grade pure. 5.3 Nitric acid solution, 1% (V/V). 5.4 Polyethylene glycol octyl phenyl ether (TritonX-100), excellent grade. 5.5 Thinner. Add 0.10 mL TritonX-100 and 0.50 mL HNO3 to 100 mL of deionized water and mix. 5.6 Aluminum standard solution. Nationally recognized standard solution. 5.7 Aluminum Standard Stock Solution and Aluminum Standard Application Solution. Accurately absorb 10.0 mL of aluminum standard solution (100.0g/mL) and place it in a 100 mL volumetric flask. Make up to the mark with nitric acid solution, this is the standard stock solution of aluminum, the concentration is 10.0g/mL, dilute the aluminum standard stock solution with diluent when it is used. 0.50 g/mL aluminum standard application solution. 6 Sample collection, transportation and storage According to the requirements of GBZ /T 295, the polyethylene plastic bottle is used to collect the urine sample of the aluminum contact worker, and after mixing, the urine specific gravity is measured as soon as possible. After adding 1 mL of nitric acid to a 100 mL urine sample, it should be transported as soon as possible at room temperature, and stored for at least 7 days at 2 °C to 6 °C.

7 Analysis steps

7.1 Instrument operating conditions The atomic absorption spectrometer was adjusted to the optimum measurement state with reference to the instrument conditions of Table 1. Table 1 Instrument operation reference conditions Instrument condition Graphite furnace condition step temperature Heating time Residence time Argon mL/min Wavelength 309.3nm Dry 1 110 5 20 250 Slit 0.7nm dry 2 130 15 30 250 Lamp current 25mA Ashing 1 1000 15 10 250 Injection volume 10μL Ashing 2 1400 10 20 250 Integral mode peak area atomization 2300 0 3 0 Graphite tube constant temperature platform cleaning 2450 1 5 250 7.2 Sample Processing Shake the urine sample, pipette the urine sample 0.20mL into the polyethylene plastic tube, add 0.80mL of diluent, mix thoroughly, to be tested; When the urine sample was replaced with 0.20 mL of deionized water, the sample was treated as the reagent blank. If the urinary aluminum content exceeds the measurement range of this law, Increase the dilution factor and measure. 7.3 Drawing of the working curve Take 7 plastic tubes with polyethylene plugs, prepare the working curve of urine aluminum according to Table 2 or prepare for automatic dilution of the instrument. Refer to the operating conditions of the instrument, the original The sub-absorption spectrophotometer was adjusted to the optimum measurement state, and 10 L was injected, and the absorbance values of the respective tubes were measured, and the absorbance of each tube was subtracted from the absorbance of the first tube. The luminosity value is the ordinate and the aluminum concentration is the abscissa, and the working curve is drawn. Table 2 Preparation of urine aluminum working curve Pipe number 1 2 3 4 5 6 7 Aluminum standard application solution/mL 0.0 0.010 0.020 0.040 0.060 0.080 0.100 Thinner/mL 0.80 0.79 0.78 0.76 0.74 0.72 0.70 Background urine/mL 0.20 0.20 0.20 0.20 0.20 0.20 0.20 Urine aluminum spiked concentration/(μg/L) 0.0 5.0 10.0 20.0 30.0 40.0 50.0 7.4 Sample determination The sample and reagent blank solution are determined by the operating conditions of the assay standard series, and the measured absorbance value of the sample is subtracted from the reagent blank absorbance value. After that, the concentration of aluminum (g/L) was obtained from the standard curve.

8 calculation

8.1 Calculate the concentration correction factor (k) for the urine sample converted to the standard specific gravity (1.020) according to formula (1). 000.1 000.1020.1 Measured specific gravity k ...(1) 8.2 Calculate the concentration of aluminum in urine according to formula (2). kFcc  0 ...(2) In the formula. C -- the concentration of aluminum in the urine, in micrograms per liter (g/L); C0--the concentration of aluminum found from the standard curve in micrograms per liter (g/L); F -- dilution ratio of urine sample; k -- The urine sample is converted to the concentration correction factor at the standard specific gravity (1.020).

9 Description

9.1 The detection limit of this method is 2.50g/L, the lower limit of quantification is 8.25g/L (5 times diluted with urine sample), and the measurement range is 8.25μg/L～ 250.0  g/L, inter-assay precision of 3.9% ~ 8.2% (urinary aluminum spiked concentration of 25.0 g/L ~.200.0  g/L, n = 6); spiked recovery It is 87.6% to 104.9% (the concentration of urinary aluminum is 25.0 g/L to.200.0 g/L, n=6). 9.2 This method should preferably use a thermostatic platform pyrolytic graphite tube or a pyrolytic coated graphite tube. The operator should be based on the performance of the instrument and graphite tube used. Adjust the instrument to its optimum working condition. 9.3 The experimental process should be strictly protected against contamination. The equipment used in the experiment was soaked with 50% HNO3 overnight and cleaned, if the aluminum blank of the experimental apparatus Still higher, it requires 6g/L of disodium edetate (Na2EDTA) solution; powder gloves are prohibited. 9.4 Using 0.10% TritonX-100 0.50% HNO3 (V/V) as a diluent, can improve the precision of the method, the diluent should be used immediately; When the urine sample is diluted at least 5 times, the precision is better; the aluminum content of the blank urine sample should not exceed 12.5 g/L. 9.5 When the aluminum concentration is 206.8g/L,.2000g/L Mg2, Na, K, Ca2, Zn2, Cd2, Pb2, Cu2, Cl-,.200g/L Ni2, Mn 2. Cr3 does not interfere with the measurement. ......

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