GB/T 9695.32-2009 English PDF

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GB/T 9695.32-2009: Meat and meat products -- Determination of chloramphenicol content
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 9695.32-2009	229	Add to Cart	3 days	Meat and meat products -- Determination of chloramphenicol content	Valid

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Basic data

Standard ID: GB/T 9695.32-2009 (GB/T9695.32-2009)
Description (Translated English): Meat and meat products -- Determination of chloramphenicol content
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: X04
Classification of International Standard: 67.040
Word Count Estimation: 10,173
Date of Issue: 2009-04-08
Date of Implementation: 2009-05-01
Regulation (derived from): National Standard Approval Announcement 2009 No.3 (Total No.143)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the method for the determination of chloramphenicol in poultry meat. This standard applies to the determination of chloramphenicol in poultry meat. This standard detection limit: gas chromatography-mass spectrometry. The detection limit was 0. 2 ��g/kg, enzyme-linked immunosorbent assay for the 0. 05��g/kg.

GB/T 9695.32-2009: Meat and meat products -- Determination of chloramphenicol content

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Meat and meat products.Determination of chloramphenicol content ICS 67.040 X04 National Standards of People's Republic of China Meat and meat products Determination of chloramphenicol Posted 2009-04-08 2009-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

The standard-setting process in reference to the "European Food Analysis CY3.6". Appendix A of this standard is an informative annex. This standard by the National Standardization Technical Committee of meat products, eggs and focal points. This standard was drafted. China Meat Research Center, China Chamber of Commerce Business Standard Centre, Wuhan Disease Control and Prevention System Center, Jiangyin City Product Quality Supervision, Xiamen Municipal Center for Disease Control. The main drafters of this standard. Song Yongqing, Zhao Rong, Liang High Road, Guo Wenping Wu Lei, Luo and East, Jin Xiaolei Liu Zhenyu. Meat and meat products Determination of chloramphenicol

1 Scope

This standard specifies the method for the determination of chloramphenicol in livestock and poultry meat. This standard applies to the determination of poultry meat chloramphenicol. The standard detection limit. gas chromatography - mass spectrometry detection limit of 0.2μg/kg; enzyme-linked immunosorbent assay to 0.05μg/kg.

2 Normative references

The following documents contain provisions by GB/T 9695 reference in this part of the provisions of this section. For dated references, All subsequent amendments (not including errata content) or revisions do not apply to this section, however, encourage the agreement on this section Whether the parties can study the latest versions of these documents. For undated reference documents, the latest versions apply to this section. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD) GB/T 9695.19 Meat and meat products - Sampling Methods 3 Gas chromatography - mass spectrometry (confirmatory method) 3.1 Principle Chloramphenicol samples extracted with ethyl acetate, n-hexane to remove the fat, the C18 purification, BSTFA + TMCS (99 + 1) derived after 3.2 Reagents and materials Unless otherwise noted, reagents were analytical grade, the water should be consistent with GB/T 6682 requirements. 3.2.1 chloramphenicol. standard, purity ≥99%. 3.2.2 Methanol. chromatographically pure. 3.2.3 chloroform. 3.2.4 n-hexane. chromatographically pure. 3.2.5 ethyl acetate. 3.2.6 anhydrous sodium sulfate. 3.2.7 sodium chloride. 3.2.8 N, O- bis trimethylsilyl trifluoroacetamide (BSTFA). 3.2.9 trimethylchlorosilane (TMCS). 3.2.10 acetone. chromatography. 3.2.11 toluene. 3.2.12 methanol solution. water + methanol = 8 + 2. 3.2.13 sodium chloride solution (40g/L). Weigh 4.00g of sodium chloride (3.2.7.7), dissolved in water, dilute to 100mL. 3.2.14 methanol - sodium chloride solution. amount of methanol solution (3.2.12) 20mL, sodium chloride solution (3.2.13) 80mL, mix. 3.2.15 mixed derivatizing agent. N, O- bis trimethylsilyl trifluoroacetamide trimethylchlorosilane + + 1 = 99. 3.2.16 chloramphenicol standard stock solution (Ba = 0.1mg/mL). Weigh chloramphenicol standard 0.01g (accurate to 0.0001g), with acetone Dissolved and set the volume to 100mL. Stock solution stored at 4 ℃ refrigerator, you can use two months. 3.2.17 Chloramphenicol standard solution. According to the test required, appropriate dilution of stock standard solution (2.2.17) with acetone (2.2.10), dubbed Standard working solution concentration. ......

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