GB/T 42879-2023 English PDF

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GB/T 42879-2023: Chemicals - Potamopyrgus antipodarum reproduction test
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Basic data

Standard ID: GB/T 42879-2023 (GB/T42879-2023)
Description (Translated English): Chemicals - Potamopyrgus antipodarum reproduction test
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: A80
Classification of International Standard: 13.300; 13.020.40
Word Count Estimation: 22,262
Date of Issue: 2023-08-06
Date of Implementation: 2023-12-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 42879-2023: Chemicals - Potamopyrgus antipodarum reproduction test

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 13.300;13.020.40 CCSA80 National Standards of People's Republic of China Chemical New Zealand Mud Snail (Potamopyrgus Published on 2023-08-06 Implemented on 2023-12-01 State Administration for Market Regulation Released by the National Standardization Administration Committee

Table of contents

Preface III Introduction IV 1 Scope 1 2 Normative reference documents 1 3 Terms and Definitions 1 4 Test principle 1 5 Test substance information 2 6 Reference 2 7 Method Description 2 8 Test procedure 4 9 Quality Assurance and Quality Control6 10 Data Processing 7 11 Report 7 Appendix A (informative) Domestication of New Zealand mud snails 9 Appendix B (informative) Pictures and software diagrams of New Zealand mud snails12 Appendix C (Informative) Sample Data Record Form 13 Appendix D (informative) Calculation of time-weighted average concentration 15 Reference 17

Foreword

This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents" Drafting. Please note that some content in this document may be subject to patents. The publisher of this document assumes no responsibility for identifying patents. This document is proposed and coordinated by the National Standardization Technical Committee for the Management of Hazardous Chemicals (SAC/TC251). This document was drafted by. Institute of Microbiology, Guangdong Academy of Sciences (Guangdong Microbial Analysis and Testing Center), Solid Waste of the Ministry of Ecology and Environment In cooperation with the Chemical Management Technology Center, China Academy of Inspection and Quarantine, Shanghai Testing Center, Xiamen Green Biotechnology Co., Ltd., Nanjing Environmental Science Institute of the Ministry of Environmental Protection, Shenyang Chemical Testing Technology (Nantong) Co., Ltd., China Chemical Information Center Co., Ltd., ChemChina Economic and Technological Development Center, Zhejiang Yawang Technology Co., Ltd. The main drafters of this document. Zeng Guoxu, Liu Chunxin, An Chao, Chen Guilan, Yang Xunan, Wang Qingbai, Liang Yanzhen, Huang Yemei, Gao Liang, Xu Meiying, Lili Zhou, Min Guo, Ying Zhang, Wei Lai, Jianwei Li, Shijie Liang, Yuyan Zhao, Zhiyong Chen, Zhesi Wang.

Introduction

potential impact on survival. A review paper on mollusk toxicity testing emphasizes the need to conduct aquatic mollusk reproduction toxicity testing for some chemicals. importance, with special emphasis on the lack of toxicity testing methods for this type of ecologically and economically important organisms. After 28 days of experimental exposure, the mortality rate was measured and the reproductive output was evaluated based on the total number of embryos produced by each female cochlea (regardless of development stage). If this method is used to test mixtures and the data obtained are used for supervision, it is necessary to consider whether the test results are sufficient and valid. If there are regulations that clearly provide for the testing of mixtures, this document can be used directly. Chemical New Zealand Mud Snail (Potamopyrgus

1 Scope

This document describes the test principles, test substance information, reference substances, method description, test procedures, Quality Assurance and Quality Control, Data and Reporting. This document applies to the New Zealand mudsnail breeding trials of chemicals.

2 Normative reference documents

The contents of the following documents constitute essential provisions of this document through normative references in the text. Among them, the dated quotations For undated referenced documents, only the version corresponding to that date applies to this document; for undated referenced documents, the latest version (including all amendments) applies to this document. GB/T 21801 Respirometry test for rapid biodegradability of chemicals GB/T 21802 MITI test for improvement of rapid biodegradability of chemicals (I) GB/T 21803 DOC reduction test for rapid biodegradability of chemicals GB/T 21856 Carbon dioxide production test for rapid biodegradability of chemicals GB/T 21857 OECD screening test for improved rapid biodegradability of chemicals

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 x% effect concentration x fectconcentration; ECx The concentration of test substance that causes a x% reduction in New Zealand mud snail reproduction during exposure. 3.2 The lowest concentration of the test substance that produces a significant effect (p < 0.05) on the test organism (reproduction and mortality) compared with the control during the exposure period. Note. All test concentrations above the LOEC produced toxic effects equal to or significantly higher than those observed in the LOEC. 3.3 The concentration of the test substance that does not produce a significant effect (p < 0.05) on the test organism compared with the control group during the exposure period. 3.4 deathmortality The subject organism ceases activity. Note. Use tweezers to gently touch the snail legs or snails of the subject creature (if the snail retracts into the volute) and no reaction is found, it is considered dead.

4 Test principle

The main purpose of the test is to evaluate chemicals by counting the number of embryos in the incubation sac after 28 days of exposure (regardless of development stage). ......

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