GB/T 40171-2021 English PDF

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GB/T 40171-2021: General rules for determination of magnetic bead DNA extraction and purification kit
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Basic data

Standard ID: GB/T 40171-2021 (GB/T40171-2021)
Description (Translated English): General rules for determination of magnetic bead DNA extraction and purification kit
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: A40
Word Count Estimation: 14,159
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 40171-2021: General rules for determination of magnetic bead DNA extraction and purification kit

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
General rules for determination of magnetic bead DNA extraction and purification kit ICS 07.080 CCSA40 National Standards of People's Republic of China General rules for the detection of magnetic bead DNA extraction and purification kits purificationkit Released on 2021-05-21 2021-12-01 implementation State Administration of Market Supervision and Administration Issued by the National Standardization Management Committee

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents" Drafting. Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents. This document was proposed and managed by the National Standardization Technical Committee for Biochemical Testing (SAC/TC387). Drafting organizations of this document. Institute of Biology, China Academy of Testing Technology, Suzhou Cretaceous Biotechnology Co., Ltd., Chengdu Customs Technology Center, Shenzhen Huada Intelligent Manufacturing Technology Co., Ltd., Shenzhen Institute of Metrology and Quality Inspection, Shengxiang Biotechnology Co., Ltd., Mike Biological Co., Ltd., Sichuan University, Beijing Physical and Chemical Analysis and Testing Center, Hunan Sunway Gene Technology Co., Ltd., Suzhou Xinbo Biotech Technology Co., Ltd. The main drafters of this document. Zhou Lihua, Ma Lixia, Lin Hua, Li Huaiping, Jiang Zijing, Lin Lin, Wang Yicong, Ye Deping, Deng Zhongping, Long Tengxiang, Jiang Zhanyue, Xie Li, Qu Feng, Jiang Hui, Yang Guowu, Gong Huafei, An Wei, Guo Gang, Zhang Jing, Dai Lizhong, Du Meihong. General rules for the detection of magnetic bead DNA extraction and purification kits

1 Scope

This document specifies the general rules for the detection of magnetic bead DNA extraction and purification kits, and describes the detection principle, preparation before detection, and the detection of magnetic beads. Method DNA extraction and purification kit magnetomechanical properties, DNA extraction and purification, DNA concentration and purity, DNA yield, DNA integrity, magnetism Bead adsorption rate, extraction recovery rate, precision detection method and test report. This document applies to plant tissues, animal tissues, blood, blood spots, blood clots, plasma, etc., saliva, semen, urine, feces and other secretions, fine Determination of magnetic bead DNA extraction kits in biological samples such as bacteria, viruses, and fungi.

2 Normative references

The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to This document. GB 5009.268 National Food Safety Standard Determination of Multi-elements in Food GB/T 19077 Particle Size Analysis Laser Diffraction Method GB/T 21649.1 Particle Size Analysis Image Analysis Method Part 1.Static Image Analysis Method GB/T 29022 Particle Size Analysis Dynamic Light Scattering Method (DLS) GB/T 34794 Agarose gel recovery kit determination general rule GB/T 34796 Determination of the concentration and purity of nucleic acids in aqueous solutions by ultraviolet spectrophotometry SN/T 2775 Evaluation Method of Commercial Food Testing Kit

3 Terms and definitions

The following terms and definitions defined in GB/T 34794 and GB/T 34796 apply to this document. 3.1 Magneticbeads A functionalized carrier whose surface has been functionally modified and coated with biologically active groups can quickly accumulate in a magnetic field and leave the magnetic field. After the field, it can quickly disperse, and realize the specific and efficient binding and dissociation of nucleic acid molecules under different conditions with superparamagnetism. Nano or micron spherical or amorphous particles. Note 1.Its surface modification has a wealth of active groups, which can be reversibly combined with nucleic acid molecules under certain buffer conditions. Note 2.Compared with traditional separation methods, magnetic beads are used for the separation of complex components of biochemical samples, which can achieve separation and enrichment at the same time, which effectively improves the separation. The separation speed and enrichment efficiency also greatly improve the sensitivity of analysis and detection. Note 3.Magnetic beads in this document refer to biological separation magnetic beads. This kind of magnetic beads has superparamagnetism and can be dispersed in the base fluid to form magnetic liquid materials and biologically active groups It can be coupled with a variety of biologically active substances, has the dual characteristics of liquid fluidity and solid magnetic particle materials, and has a definite particle size Scope, there are certain surface characteristics, including specific surface area, surface modification, etc. 3.2 Adsorption rateofmagneticbeads Under certain conditions, the ratio of nucleic acid adsorbed by magnetic beads to the total amount of nucleic acid. ......

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