GB/T 40170-2021 English PDF

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GB/T 40170-2021: Principle of plasmid extraction and testing
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Basic data

Standard ID: GB/T 40170-2021 (GB/T40170-2021)
Description (Translated English): Principle of plasmid extraction and testing
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: A40
Word Count Estimation: 18,137
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 40170-2021: Principle of plasmid extraction and testing

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Principle of plasmid extraction and testing ICS 07.080 CCSA40 National Standards of People's Republic of China General Rules for Plasmid Extraction and Testing Released on 2021-05-21 2021-12-01 implementation State Administration of Market Supervision and Administration Issued by the National Standardization Management Committee

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents" Drafting. This document was proposed and managed by the National Standardization Technical Committee for Biochemical Testing (SAC/TC387). Drafting organizations of this document. Institute of Biology, China Academy of Testing Technology, General Biological System (Anhui) Co., Ltd., Shenzhen Analysis and Testing Test Association, Gansu Zhongshang Food Quality Inspection and Testing Co., Ltd., Shenzhen Second People’s Hospital, Chengdu Institute of Food and Drug Inspection, Gansu Provincial Commercial Science and Technology Research Institute Co., Ltd. The main drafters of this document. Zhou Lihua, Yong Jingui, Wang Lina, Yu Mingjun, Cui Kangle, Ma Lixia, Luo Xiaowen, Jiang Zijing, Wang Weikun, Gu Dayong, Ye Deping, Yang Jie, He Haining. General Rules for Plasmid Extraction and Testing

1 Scope

This document specifies the terms and definitions, abbreviations, principles, extraction, testing, quality control, sample preservation, and reporting of plasmid extraction and testing And waste disposal. This document is suitable for the extraction and testing of plasmids.

2 Normative references

The contents of the following documents constitute indispensable clauses of the documents through normative references in the text. Among them, the dated reference documents, only The version corresponding to this date is applicable to this document; for undated references, the latest version (including all amendments) is applicable to this document. GB 4789.3 National Food Safety Standard Food Microbiological Inspection Coliform Count GB/T 19495.1 General requirements and definitions for the testing of genetically modified products GB/T 19495.2 Technical requirements for genetically modified products testing laboratories GB/T 27403 Laboratory Quality Control Specification Food Molecular Biology Testing GB/T 34265 Sanger method sequencing technology guide GB/T 34796 Determination of the concentration and purity of nucleic acids in aqueous solutions by ultraviolet spectrophotometry

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Plasmid One or more closed circular double-stranded DNA molecules other than chromosomes (or nucleoids) in organisms such as bacteria, yeasts, and actinomycetes. Note. It exists in the cytoplasm and has the ability to replicate independently, enabling it to maintain a constant copy number in the progeny cells and express the genetic information it carries. 3.2 Ribonuclease A RNaseA A kind of 3'and 5'phosphodiester bonds of the ribose moiety that catalyzes the hydrolysis of ribonucleic acid to form oligonucleosides with 2', 3'-cyclic monophosphate derivatives Acid nuclease. 3.3 Bacterialendotoxin A complex composed of hydrophilic heteropolysaccharides and a covalently bound lipid A. Note. The complex is located on the cell wall of gram-negative bacteria. Suction distress, intravascular coagulation and endotoxin shock syndrome.

4 Abbreviations

The following abbreviations apply to this document. DNA. Deoxyribonucleic Acid (Deoxyribonucleic Acid) ......

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