GB/T 39649-2020 English PDF

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GB/T 39649-2020: Laboratory animal - Quality control of laboratory fish
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Basic data

Standard ID: GB/T 39649-2020 (GB/T39649-2020)
Description (Translated English): Laboratory animal - Quality control of laboratory fish
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B44
Classification of International Standard: 65.020.30
Word Count Estimation: 24,239
Date of Issue: 2020-12-14
Date of Implementation: 2020-12-14
Quoted Standard: GB 5749; GB/T 5750.1; GB/T 5750.2; GB/T 5750.3; GB/T 5750.4; GB/T 5750.5; GB/T 5750.6; GB/T 5750.7; GB/T 5750.8; GB/T 5750.9; GB/T 5750.10; GB/T 5750.11; GB/T 5750.12; GB/T 5750.13; GB 8978; GB 14925; GB/T 18652; GB/T 18654.3; GB/T 19495.3; NY 5052
Regulation (derived from): National Standard Announcement No. 28 of 2020
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration
Summary: This standard specifies the quality control and monitoring methods of experimental fish germplasm, genetics, microorganisms and parasites, feed, and environmental facilities. This standard applies to the quality control of zebrafish (Danio rerio), swordtail (Xiphophorus helleri) and Mugilogobius chulae (Mugilogobius chulae) germplasm, genetics, microorganisms and parasites, feed and environmental facilities.

GB/T 39649-2020: Laboratory animal - Quality control of laboratory fish

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Experimental animals Experimental fish quality control) ICS 65:020:30 B44 National Standards of People's Republic of China Quality control of experimental fish 2020-12-14 release 2020-12-14 implementation State Administration for Market Regulation Issued by the National Standardization Management Committee

Table of contents

Preface Ⅲ 1 Scope 1 2 Normative references 1 3 Terms and definitions 1 4 Germplasm identification 2 5 Genetic management 3 6 Microorganism and Parasite Monitoring 4 7 Feed 5 8 Environmental facilities 6 Appendix A (Normative Appendix) Experimental fish DNA barcode primers and sequences 9 Appendix B (Informative Appendix) Identification of Parasites in Experimental Fish 11 Appendix C (Normative appendix) Isolation, cultivation and identification of experimental fish microorganisms 13 Appendix D (informative appendix) Paramecium breeding and feeding 16 Appendix E (informative appendix) Breeding and feeding of Brachionus plicatilis 17 Appendix F (informative appendix) Artemia hatching and feeding 18 Appendix G (informative appendix) Experimental fish stocking density 19 Appendix H (informative appendix) Test method for environmental indicators of experimental fish 20 Reference 21

Foreword

This standard was drafted in accordance with the rules given in GB/T 1:1-2009: Please note that certain contents of this document may involve patents: The issuing agency of this document is not responsible for identifying these patents: This standard was proposed and managed by the National Laboratory Animal Standardization Technical Committee (SAC/TC281): Drafting organizations of this standard: Guangdong Laboratory Animal Monitoring Institute, Institute of Hydrobiology, Chinese Academy of Sciences, Pearl River, Chinese Academy of Fishery Sciences Fisheries Research Institute, Shanghai Laboratory Animal Research Center, Institute of Medical Laboratory Animals, Chinese Academy of Medical Sciences: The main drafters of this standard: Huang Ren, Sun Yonghua, Li Kaibin, Hu Jianhua, Li Jianjun, Yu Lujun, Cai Lei, Liu Yunbo, Lin Jinxing, Pan Luhuan, Wu Shuqin and Chen Meili: Quality control of experimental fish

1 Scope

This standard specifies the quality control and monitoring methods of experimental fish germplasm, genetics, microorganisms and parasites, feed, and environmental facilities: This standard applies to zebrafish (Daniorerio), swordtail fish (Xiphophorushelleri) and mullet goby (Mugilogobius chulae) germplasm, genetics, microorganisms and parasites, feed, quality control of environmental facilities:

2 Normative references

The following documents are indispensable for the application of this document: For dated reference documents, only the dated version applies to this article Pieces: For undated references, the latest version (including all amendments) applies to this document: GB 5749 Sanitary Standard for Drinking Water GB/T 5750 (all parts) Standard inspection method for drinking water GB 8978 Integrated Wastewater Discharge Standard GB 14925 Laboratory Animal Environment and Facilities GB/T 18652 Test method for pathogenic Aeromonas hydrophila GB/T 18654:3 Germplasm Inspection of Farmed Fish Part 3: Determination of Characters GB/T 19495:3 Method for extraction and purification of nucleic acid for detection of genetically modified products NY5052 pollution-free food mariculture water quality SC/T 6040 Safety and Health Requirements for Aquatic Products Factory Farming Equipment SC/T 7214:1 Method for detection of Edwardsiella in fish Part 1: Edwardsiella lentus

3 Terms and definitions

The following terms and definitions apply to this document: 3:1 Laboratoryfish After artificial breeding, its genetics, microorganisms, parasites, feed and environmental facilities are controlled for scientific research, teaching, production, inspection Fish for testing, identification and other scientific experiments: 3:2 Finformula A formula that expresses the composition and structure of fins and the types and numbers of fins: Note: Generally, "D:" represents the dorsal fin, "A:" represents the anal fin, "P:" represents the pectoral fin, "V:" represents the pelvic fin, and "C:" represents the caudal fin; the number of fin spines is in capital Roman letters Indicates that the number of unbranched fins is represented by lowercase Roman numerals, and the number of branched fins is represented by Arabic numerals; the number range of spines or fins is represented by "~" When the spines are connected with the fin rays, it is indicated by "-", and when they are separated by ",": 3:3 Livingfeeds Artificially screened organisms that can be cultivated artificially and are suitable for feeding by breeding objects: ......

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