GB/T 39602-2020 English PDF

US$359.00 · In stock
Delivery: <= 4 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 39602-2020: Diagnostic techniques for Lumpy skin disease
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 39602-2020	359	Add to Cart	4 days	Diagnostic techniques for Lumpy skin disease	Valid

Similar standards

GB/T 25165   NY/T 1663   GB/T 20365   GB/T 26436   GB/T 18936   

Basic data

Standard ID: GB/T 39602-2020 (GB/T39602-2020)
Description (Translated English): Diagnostic techniques for Lumpy skin disease
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 19,112
Date of Issue: 2020-12-14
Date of Implementation: 2020-12-14
Quoted Standard: GB/T 6682; GB 19489; NY/T 541
Regulation (derived from): National Standard Announcement No. 28 of 2020
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration
Summary: This standard specifies the clinical diagnosis, laboratory diagnostic techniques and procedures for bovine nodular skin disease (LSD). This standard applies to the diagnosis of bovine nodular skin diseases. Virus isolation and identification is suitable for the no-infection certificate or clinical case confirmation before the movement of individual animals; electron microscope observation is suitable for the diagnosis of clinical cases; real-time fluorescent PCR and ordinary PCR are suitable for the no-infection certificate, clinical case confirmation and monitoring of infection before the movement of individual animals Prevalence rate; Serum neutralization test is suitable for proof of no infection, confirmed clinical cases, monitoring infection prevalence rate and immune effect evaluation before individual animals move.

GB/T 39602-2020: Diagnostic techniques for Lumpy skin disease

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(The diagnosis technology of bovine nodular skin disease) ICS 11:220 B41 National Standards of People's Republic of China The diagnosis technology of bovine nodular skin disease 2020-12-14 release 2020-12-14 implementation State Administration for Market Regulation Issued by the National Standardization Management Committee

Table of contents

Preface Ⅲ Introduction Ⅳ 1 Scope 1 2 Normative references 1 3 Abbreviations 1 4 Clinical diagnosis 1 4:1 Susceptible animals 1 4:2 Clinical symptoms 2 4:3 Pathological changes 2 4:4 Results judgment 2 5 Laboratory diagnostic sample collection 2 5:1 Equipment 2 5:2 Reagent 2 5:3 Sample collection 3 5:4 Inspection and storage of samples 3 5:5 Sample processing 3 6 Electron microscope observation 4 6:1 Equipment 4 6:2 Reagent 4 6:3 Step 4 6:4 Results judgment 4 7 Virus isolation and identification 4 7:1 Equipment 4 7:2 Cells and reagents 4 7:3 Virus isolation 5 7:4 Virus identification 5 8 Real-time fluorescent polymerase chain reaction (real-time fluorescent PCR) 5 8:1 Equipment 5 8:2 Reagent 5 8:3 Primers and probes 5 8:4 Standard strains and cells 6 8:5 Processing of samples 6 8:6 Extraction and purification of viral DNA 6 8:7 Real-time fluorescent PCR detection 6 8:8 Results judgment 7 9 Polymerase chain reaction (general PCR method) 7 9:1 Equipment 7 9:2 Reagent 7 9:3 Primer 8 9:4 Preparation of samples 8 9:5 PCR detection 8 9:6 Results judgment 9 10 Minimal Neutralization Test (VN) 9 10:1 Equipment 9 10:2 Cell line 9 10:3 Standard strain 9 10:4 Operation steps 9 10:5 Negative and positive control 10 10:6 Result judgment 10 10:7 Interpretation of results 10 11 Comprehensive decision 10 11:1 Suspected 10 11:2 Confirmed 10 Appendix A (Normative Appendix) Reagent Preparation 11 Appendix B (informative appendix) primer amplification sequence 14

Foreword

This standard was drafted in accordance with the rules given in GB/T 1:1-2009: This standard corresponds to 3:4:12 bovine nodular skin in the latest OIE's "Manual of Diagnostic Tests and Vaccines for Terrestrial Animals" (2018 Edition) Disease (LSD) related content, and the degree of consistency with the standard is not equivalent: This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China: This standard is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181): Drafting organizations of this standard: Chongqing Customs of the People's Republic of China, China Animal Health and Epidemiology Center, Shanghai, People's Republic of China Customs, Chongqing Aolong Biological Products Co:, Ltd: The main drafters of this standard: Nie Fuping, Li Lin, Li Yingguo, Wu Xiaodong, Wang Yu, Yang Jun, Fan Xiaoxu, Wang Guomin, Li Xianliang, Nan Wenlong, Shi Meimei, Zhang Lei, Zou Yanli, Wang Zhiliang, Li Jian, Ran Zhiguang:

Introduction

The issuing agency of this document draws attention to the fact that when declaring compliance with this document, primer pair 2 and probe 2 in 8:3 primers and probes may be involved Comparing with "Primers, Kits and Detection Methods for Bovine Nodular Skin Disease Virus Field Strain TaqMan-MGB Real-time Fluorescent Quantitative PCR Detection" Use of related patents: The issuing agency of this document has no position on the authenticity, validity and scope of the patent: The patent holder has assured the issuing organization of this document that he is willing to work with any applicant under reasonable and non-discriminatory terms and conditions: Negotiations on patent licensing: The statement of the patent holder has been filed with the issuing agency of this document: Relevant information can be through the following Contact information: Names of patent holders: Nie Fuping, Wang Yu, Yang Jun, Li Xianliang, Wang Guomin, Li Yingguo: Address: No: 8 Honghuang Road, Jiangbei District, Chongqing: Please note that in addition to the above patents, certain contents of this document may still involve patents: The issuing agency of this document is not responsible for identifying these Li’s responsibility: The diagnosis technology of bovine nodular skin disease

1 Scope

This standard specifies the clinical diagnosis and laboratory diagnostic techniques and procedures for bovine nodular skin disease (LSD): This standard applies to the diagnosis of bovine nodular skin diseases: Virus isolation and identification is suitable for the non-infection certificate or clinical Confirmation of bed cases; Electron microscope observation is suitable for diagnosis of clinical cases; Real-time fluorescent PCR and ordinary PCR are suitable for the insensitivity of individual animals before moving Infection certificate, clinical case diagnosis and monitoring of infection prevalence; serum neutralization test is suitable for the non-infection certificate and clinical diagnosis of individual animals before moving Bed cases, monitoring the prevalence of infection and evaluation of the immune effect:

2 Normative references

The following documents are indispensable for the application of this document: For dated reference documents, only the dated version applies to this article Pieces: For undated references, the latest version (including all amendments) applies to this document: GB/T 6682 Analytical laboratory water specifications and test methods GB 19489 Laboratory Biosafety General Requirements NY/T 541 Technical Specification for Collection, Storage and Transportation of Veterinary Diagnostic Samples

3 Abbreviations

The following abbreviations apply to this document: CPE: Cytopathiceffect DNA: Deoxyribonucleic acid (Deoxyribonucleicacid) EDTA: EthyleneDiamineTetraacetic Acid (EthyleneDiamineTetraacetic Acid) GMEM: Growth nutrient requirement medium (Glasgow'sModifiedEagle'sMedium) LSD: bovine nodular skin disease (Lumpyskindisease) LSDV: Bovine Nodular Skin Disease Virus (Lumpyskindiseasevirus) LTc: Lambtestiscel ORF: Open reading frame (Openreadingframe) PBS: Phosphate buffered saline (Phosphatebufferedsaline) PCR: Polymerase chain reaction (Polymerasechain reaction) TCID50: half of tissue culture infection (Mediantissuecultureinfectivedose) Vero: African green monkey kidney cells (Africangreenmonkeykidneycel) VN: Virus neutralisation test (Virusneutralisationtest)

4 Clinical diagnosis

4:1 Susceptible animals All kinds of cattle, including yellow cattle, dairy cows, Asian buffalo, zebu, yak are susceptible, sheep, goats and wild animals giraffes, black spots Antelope and antelope can be infected artificially: ......

Image     

Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 39602-2020_English be delivered?

Answer: Upon your order, we will start to translate GB/T 39602-2020_English as soon as possible, and keep you informed of the progress. The lead time is typically 2 ~ 4 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 39602-2020_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 39602-2020_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.