GB/T 39100-2020 English PDF

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GB/T 39100-2020: Determination of antioxidant activity for polypeptides - DPPH and ABTS methods
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 39100-2020	189	Add to Cart	3 days	Determination of antioxidant activity for polypeptides - DPPH and ABTS methods	Valid

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Basic data

Standard ID: GB/T 39100-2020 (GB/T39100-2020)
Description (Translated English): Determination of antioxidant activity for polypeptides - DPPH and ABTS methods
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: A40
Classification of International Standard: 07.080
Word Count Estimation: 10,137
Date of Issue: 2020-09-29
Date of Implementation: 2021-04-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 39100-2020: Determination of antioxidant activity for polypeptides - DPPH and ABTS methods

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of antioxidant activity for polypeptides - DPPH and ABTS methods ICS 07.080 A40 National Standards of People's Republic of China Determination of Antioxidant Properties of Peptides by DPPH and ABTS Methods 2020-09-29 released Implementation on 2021-04-01 State Administration for Market Regulation Issued by the National Standardization Management Committee

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed and managed by the National Standardization Technical Committee for Biochemical Testing (SAC/TC387). Drafting organizations of this standard. Hebei University of Science and Technology, Hebei Agricultural University, Beijing Technology and Business University, China Testing and Certification Group Co., Ltd., Beijing Sambo Technology Co., Ltd., Wuhan Mingliang Biotechnology Co., Ltd., Zhejiang Dongjie Biotechnology Co., Ltd., market supervision and management Food Evaluation Center of General Administration of Science and Technology, China Academy of Inspection and Quarantine. The main drafters of this standard. Wang Zhixin, Tian Yiling, Jia Yingmin, Ma Aijin, Hao Shuai, Peng Hai, Liu Wenqiu, Yang Zhijian, Zheng Gang, Jiang Yu, Zou Mingqiang, Sun Yu, Qi Xiaohua. Determination of Antioxidant Properties of Peptides by DPPH and ABTS Methods

1 Scope

This standard specifies the methods of DPPH and ABTS methods to determine the antioxidant properties of peptides. This standard is applicable to the determination of the in vitro antioxidant properties of peptides.

2 Normative references

The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this article Pieces. For undated references, the latest version (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Polypeptides Between amino acids and proteins, a class of compounds formed by two or more amino acids connected by peptide bonds. 3.2 Antioxidantactivity The ability to scavenge free radicals is expressed as the ratio (AO value) of the half-clearance of the tested polypeptide to the half-clearance of glutathione.

4 Abbreviations

The following abbreviations apply to this document. ABTS. 2,2-Azino-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt [2,2'-azino-bis(3-ethylbenzothiazoline-6- sulfonicacid)diammoniumsalt] AO. antioxidantactivity DPPH. 1,1-diphenyl-2-picrylhydrazyl (1,1-diphenyl-2-picrylhydrazyl) DMSO. dimethylsulfoxide (dimethylsulfoxide) EC50.half maximum clearance (halfmaximaleffectiveconcentration)

5 Reagents or materials

Unless otherwise specified, the reagents used in this method are analytically pure, and the test water is the first grade water specified in GB/T 6682. 5.1 Glutathione L-reduced type, purity ≥98%. ......

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