GB/T 36849-2018 English PDF

US$279.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 36849-2018: Detection and identification of Coleus blumei viroids
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 36849-2018	279	Add to Cart	3 days	Detection and identification of Coleus blumei viroids	Valid

Similar standards

GB/T 43165   GB/T 40135   GB/T 19495.4   GB/T 36840   GB/T 36839   

Basic data

Standard ID: GB/T 36849-2018 (GB/T36849-2018)
Description (Translated English): Detection and identification of Coleus blumei viroids
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 14,132
Date of Issue: 2018-09-17
Date of Implementation: 2019-04-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 36849-2018: Detection and identification of Coleus blumei viroids

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Coleus blumei viroids ICS 65.020.01 B16 National Standards of People's Republic of China Multicolored su virus quarantine and identification method Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271). This standard was drafted. China Academy of Inspection and Quarantine, Xiamen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, China Agricultural Division Institute of Plant Protection, Qingdao Academy of Agricultural Sciences. The main drafters of this standard. Zhang Yongjiang, Xiang Jun, Liao Furong, Xin Yanyan, Li Shihan, Ma Rongqun. Multicolored su virus quarantine and identification method

1 Scope

This standard specifies the quarantine and identification methods for multicolored threoviruses. This standard applies to the quarantine and identification of colorful Su.

2 Basic information

Chinese name. Colorful Su virus Scientific name. Coleusblumeiviroid Abbreviation. CbVd Synonym. Coleus Taxonomic status. Pospiviroidae, a genus of the genus Coleviroid. See Appendix A for additional information on the colorful Su-like virus.

3 Principle of the method

The difference in mobility between polyacrylamide gel electrophoresis based on the natural state of CbVd genomic RNA and different structural molecules after denaturation Regression-reversible polyacrylamide gel electrophoresis (R-PAGE); The specificity of the nucleic acid sequence was subjected to reverse transcription polymerase chain reaction (RT-PCR), and the result was determined by the size of the electrophoresis band. 4 Instruments, equipment and reagents 4.1 Instrumentation Electronic analytical balance (0.001g), vertical electrophoresis tank, small centrifuge, bench-top refrigerated centrifuge, ordinary PCR instrument, electrophoresis system, pH meter, gel imaging system, 4°C refrigerator, ultra-clean workbench, -80°C ultra-low temperature refrigerator, autoclave, ice machine, vortex oscillator, micro Wave furnace, etc. 4.2 Appliances Adjustable pipettes (2.5μL, 10μL, 20μL, 100μL, 1000μL), tips, centrifuge tubes (0.2mL, 0.5mL, 1.5mL) and research Hey, etc. 4.3 Reagents All experimental reagents were of analytical or biochemical reagents unless otherwise stated. See Appendix B for R-PAGE reagents and Appendix C for RT-PCR reagents.

5 Detection methods

5.1 R-PAGE detection See Appendix B for R-PAGE detection. ......

Image     

Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 36849-2018_English be delivered?

Answer: Upon your order, we will start to translate GB/T 36849-2018_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 36849-2018_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 36849-2018_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.