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GB/T 36846-2018 English PDF

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US$359.00 · In stock
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GB/T 36846-2018: Detection and identification of Potato virus M
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 36846-2018	359	Add to Cart	4 days	Detection and identification of Potato virus M	Valid

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Basic data

Standard ID: GB/T 36846-2018 (GB/T36846-2018)
Description (Translated English): Detection and identification of Potato virus M
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 18,122
Date of Issue: 2018-09-17
Date of Implementation: 2019-04-01
Regulation (derived from): National Standard Announcement No. 11 of 2018
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 36846-2018: Detection and identification of Potato virus M

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Potato virus M ICS 65.020.01 B16 National Standards of People's Republic of China Potato M virus quarantine and identification method Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. Publication of this document The organization is not responsible for identifying these patents. This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271). This standard was drafted. Heilongjiang Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Zhongshan Entry and Exit Inspection and Quarantine of the People's Republic of China Bureau, China Academy of Inspection and Quarantine, Shandong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Zhejiang University. The main drafters of this standard. Liu Zhongmei, Liu Hongyi, Ma Wei, Chen Dinghu, Yan Fengxia, Zhang Yongjiang, Feng Liping, Li Mingfu, Wu Jianxiang, Li Guifen, Wei Meisheng, Yang Liqun. Potato M virus quarantine and identification method

1 Scope

This standard specifies the serological and molecular biological detection methods for quarantine identification of potato M virus. This standard applies to the quarantine and identification of potato plants, propagation materials and other host plants that may have potato M virus.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB 7331 Potato seed potato quarantine regulations SN/T 2122 Quarantine sampling method for inbound and outbound plants and plant products

3 potato M virus basic information

Chinese name. Potato M virus Scientific name. PotatovirusM Abbreviation. PVM Synonym. Kartoffel-Rolmosaikvirus, Ksrtoffel-K-virus, Pototainterveinalmosaicvirus, Potatoleaf Rolingmosaicvirus, PotatoMcarlavirus, potatoparacrinklevirus, PotatovirusE, Solanumvirus 11, Solanumvirus7 Classification status. Betaflexiviridae, Carlavirus. Route of transmission. The virus can be transmitted by mechanical inoculation and non-persistent transmission of aphids, or through seed potatoes, commercial potatoes, tissue culture seedlings, etc. The material is spread over long distances. See Appendix A for additional information on the potato M virus.

4 Principle of the method

The immunogenicity and genomic characteristics of PVM are the basis for the quarantine and identification of this virus. Establishment of enzyme-linked immunosorbent assay based on PVM immunogenicity Attached assay (ELISA) method and dot enzyme-linked immunosorbent assay (dot-ELISA) method; reverse transcription based on genomic features of PVM PCR (RT-PCR) and reverse transcription loop-mediated isothermal amplification (RT-LAMP) detection methods. 5 Instruments, equipment and reagents 5.1 Instrumentation Microplate reader, PCR instrument, electrophoresis instrument, biological safety cabinet, small centrifuge, desktop refrigerated centrifuge, constant temperature water bath, pH meter, gel imaging System, ice machine, ultra-clean workbench, autoclave, low temperature refrigerator, electronic balance (0.001g), vortex oscillator, constant temperature incubator, etc. 5.2 Appliances Adjustable pipettes (2.5μL, 10μL, 20μL, 100μL, 1000μL), tips, centrifuge tubes and mortars. ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 36846-2018_English be delivered?

Answer: Upon your order, we will start to translate GB/T 36846-2018_English as soon as possible, and keep you informed of the progress. The lead time is typically 2 ~ 4 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 36846-2018_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 36846-2018_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.