GB/T 36815-2018 English PDF

US$359.00 · In stock
Delivery: <= 4 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 36815-2018: Detection and identification of Diaporthe vaccinii Shear
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 36815-2018	359	Add to Cart	4 days	Detection and identification of Diaporthe vaccinii Shear	Valid

Similar standards

GB/T 43165   GB/T 40135   GB/T 19495.4   GB/T 36807   GB/T 36806   

Basic data

Standard ID: GB/T 36815-2018 (GB/T36815-2018)
Description (Translated English): Detection and identification of Diaporthe vaccinii Shear
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 18,164
Date of Issue: 2018-09-17
Date of Implementation: 2019-04-01
Regulation (derived from): National Standard Announcement No. 11 of 2018
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 36815-2018: Detection and identification of Diaporthe vaccinii Shear

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Diaporthe vaccinii Shear ICS 65.020.01 B16 National Standards of People's Republic of China Method for quarantine and identification of blueberry rot Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271). This standard was drafted. Tianjin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Luo Jiafeng, Cui Tiejun, Liu Yueting, Zhang Ying, Huang Guoming, Liao Fang. Method for quarantine and identification of blueberry rot

1 Scope

This standard specifies the isolation culture, pathogenicity determination and molecular biological detection methods of blueberry fruit rot. This standard is applicable to the quarantine and identification of blueberry fruit rot fungi in host seedlings, plants and fruits of blueberry fruit rot.

2 Basic information of Raspberry rot

Chinese name. Blueberry fruit rot Scientific name. DiaporthevacciniiShear Asexual name. PhomopsisvacciniiShearN.E.Stevens English name. fruitrotofblueberry, Phomopsiscankerofblueberry, uprightdiebackofcranberry Classification status. Diaporthevaccinii Shear belongs to fungi (Fungi), Ascomycota, Ascomycetes, Diaporthales, Diapothaceae, Diaporthe. Route of transmission. The pathogens rely on the seedlings and fruits for long-distance transmission. See Appendix A for additional information on Raspberry rot.

3 Principle of the method

Pathogenic bacteria, isolation and culture traits, morphological characteristics, pathogenicity determination (see Appendix A, Appendix B, Appendix C) and ITS1/ The results of ITS4 nucleic acid sequence alignment were used as the basis for quarantine identification of blueberry fruit rot.

4 instruments and main reagents

4.1 Apparatus 4.1.1 Instruments Stereo microscope, biological microscope, ultra-clean workbench, biological incubator, electronic balance, autoclave, conventional refrigerator (-20 ° C), PCR amplification instrument, electrophoresis instrument, gel imager, high speed refrigerated centrifuge, constant temperature water bath. 4.1.2 Appliances Sterilized culture dish (diameter 90mm~100mm), triangle bottle, tweezers, surgical scissors, scalpel, inoculation needle, disposable syringe (1mL), cotton yarn, absorbent cotton, alcohol lamp, plastic mortar, adjustable micro sampler. 4.2 Primary reagents Ethylenediaminetetraacetic acid (EDTA), sodium dodecyl sulfate (SDS), Tris-HCl, chloroform, isoamyl alcohol, chloroform, ethanol, potassium chloride, Molecular biological reagents such as magnesium chloride, proteinase K, primers ITS1/ITS4, Taq polymerase, or plant tissue and fungal genome extraction The kit extracts DNA. Glucose, agar, penicillin, streptomycin, lactic acid, 2.0% sodium hypochlorite (NaClO). Acid potato glucose medium (APDA, pH 5.0~6.0), APDA selective medium (see Appendix D for preparation methods). ......

Image     

Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 36815-2018_English be delivered?

Answer: Upon your order, we will start to translate GB/T 36815-2018_English as soon as possible, and keep you informed of the progress. The lead time is typically 2 ~ 4 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 36815-2018_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 36815-2018_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.