GB/T 36808-2018 English PDF

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GB/T 36808-2018: Detection and identification of Xanthomonas cassavae (ex Wiehe et Dowson) Vauterin et al.
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 36808-2018	279	Add to Cart	3 days	Detection and identification of Xanthomonas cassavae (ex Wiehe et Dowson) Vauterin et al.	Valid

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Basic data

Standard ID: GB/T 36808-2018 (GB/T36808-2018)
Description (Translated English): Detection and identification of Xanthomonas cassavae (ex Wiehe et Dowson) Vauterin et al.
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 14,197
Date of Issue: 2018-09-17
Date of Implementation: 2019-04-01
Regulation (derived from): National Standard Announcement No. 11 of 2018
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 36808-2018: Detection and identification of Xanthomonas cassavae (ex Wiehe et Dowson) Vauterin et al.

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Xanthomonas cassavae (ex Wiehe et Dowson) Vauterin et al. ICS 65.020.01 B16 National Standards of People's Republic of China Method for quarantine and identification of cassava bacterial leaf spot bacteria Vauterinetal. Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271). This standard was drafted. Shandong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Huangdao Entry-Exit Inspection and Quarantine Bureau, Linyi Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Feng Liping, Li Yan, Guan Xufang, Zhao Liqing, Xia Mingxing, Lunzhizhi, Wang Jian, Wang Yingchao, Wu Xinghai, Shao Xiuling. Method for quarantine and identification of cassava bacterial leaf spot bacteria

1 Scope

This standard specifies the quarantine and identification methods for cassava bacterial leaf spot in plant quarantine. This standard applies to the quarantine and identification of cassava bacterial leaf spot bacteria in the middle leaves, aboveground stems, seedlings, trunks and branches of cassava propagation materials.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 28096-2011 Method for quarantine and identification of cassava bacterial wilt disease

3 Basic information of cassava bacterial leaf spot pathogen

3.1 Name Scientific name. Xanthomonascassavae (exWieheetDowson) Vauterinetal. Synonym. Xanthomonascampestrispv.cassavae (Wiche English name. Cassavalaefspot 3.2 Classification status Cassava Bacteria, Bacteria, Proteobacteria, Proteobacteria Gammaproteobacteria, Yellow Xanthomonadales, Xanthomonadaceae, Xanthomonas.

4 Principle of the method

Cassava bacterial leaf spot disease infects cassava (Manihotesculenta). It is mainly transmitted through diseased roots, leaves, etc. Point (see Appendix A), culture traits and biological characteristics (see Appendix A), pathogenicity determination, molecular biological characteristics of pathogens are the pathogens Basis for identification.

5 instruments

5.1 Electronic balance. 5.2 Biological microscope. magnification 40 × ~ 1000 ×. 5.3 Biological safety cabinet. in accordance with EN12469.2000. 5.4 Temperature control incubator. temperature control range 5 ° C ~ 50 ° C. 5.5 Autoclave. Sterilization working temperature is 105 °C ~ 138 °C. 5.6 PCR instrument. control accuracy ± 0.25 ° C, temperature range 4 ° C ~ 99 ° C. 5.7 Agarose level electrophoresis apparatus. 5.8 Gel Imaging Analysis System. ......

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