GB/T 36752-2018 English PDF

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GB/T 36752-2018: Detection and identification of Sowbane mosaic virus
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 36752-2018	279	Add to Cart	3 days	Detection and identification of Sowbane mosaic virus	Valid

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Basic data

Standard ID: GB/T 36752-2018 (GB/T36752-2018)
Description (Translated English): Detection and identification of Sowbane mosaic virus
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 14,182
Date of Issue: 2018-09-17
Date of Implementation: 2019-04-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 36752-2018: Detection and identification of Sowbane mosaic virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Valerian mosaic virus quarantine and identification method) ICS 65.020.01 B16 National Standards of People's Republic of China Valerian mosaic virus quarantine and identification method Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271). This standard was drafted. China Academy of Inspection and Quarantine, Guangdong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, the Chinese people Hezhou Xiamen Entry-Exit Inspection and Quarantine Bureau, Zhejiang University. The main drafters of this standard. Zhang Yongjiang, Feng Lixia, Xiang Jun, Liao Furong, Wu Jianxiang, Xin Yanyan, Li Guifen, Chen Qing. Valerian mosaic virus quarantine and identification method

1 Scope

This standard specifies the serological and molecular biological detection methods for quarantine identification of valerian mosaic virus. This standard applies to the quarantine and identification of plant seeds, seedlings and plant products that may have valerian mosaic virus.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods SN/T 2122 Quarantine sampling of inbound and outbound plants and plant products

3 Herba Mosaic Virus Basic Information

Scientific name. Sowbanemosaicvirus Abbreviation. SoMV Classification status. members of the southern bean genus Sobemovirus See Appendix A for additional information on Valerian mosaic virus.

4 Principle of the method

The serological and genomic characteristics of Valerian mosaic virus is the basis for the quarantine and identification of the virus. According to the relationship between SoMV and antibody Specific reaction, double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for plant samples; based on genomic characteristics of SoMV RT-PCR and real-time fluorescent RT-PCR detection methods were established; through the effective combination of these methods, the samples were judged to have SoMV.

5 instruments and reagents

5.1 Instrument Electronic balance (sensitivity 0.001g). High-speed refrigerated centrifuge (maximum speed 15000r/min). Small instantaneous centrifuge (maximum speed 7000r/min). Ordinary refrigerator (4 ° C). Ultra-low temperature refrigerator (-80 ° C). Ice machine (daily ice making capacity is 110kg). Vortex oscillator (maximum speed is 2800r/min). Magnetic stirrer (stirring capacity 50mL~2000mL). Autoclave (maximum pressure is 0.235MPa, adjustable temperature range is 105 °C ~ 135 °C). pH meter (range pH 0.00~14.00, accuracy ±0.01pH). ......

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