GB/T 36389-2018 English PDF

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GB/T 36389-2018: T4 DNA ligase
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 36389-2018	199	Add to Cart	3 days	T4 DNA ligase	Valid

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Basic data

Standard ID: GB/T 36389-2018 (GB/T36389-2018)
Description (Translated English): T4 DNA ligase
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: C27
Classification of International Standard: 07.080
Word Count Estimation: 10,154
Date of Issue: 2018-06-07
Date of Implementation: 2019-01-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 36389-2018: T4 DNA ligase

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
T4 DNA ligase ICS 07.080 C27 National Standards of People's Republic of China T4 DNA ligase Published on.2018-06-07 2019-01-01 implementation State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is under the jurisdiction of the National Tool Enzyme Standardization Working Group (SAC/SWG11). This standard was drafted. Fujian Nansheng Technology Co., Ltd., Fujian Huacan Pharmaceutical Co., Ltd., Suzhou Beaver Biomedical Engineering Co., Ltd. Division, Xiamen Zhishan Biotechnology Co., Ltd., Xiamen University, Angel Yeast Co., Ltd. The main drafters of this standard. Zheng Dengzhong, Zhan Xuexiong, Huang Facan, Ren Hui, Zhao Jing, Zhang Lili, Song Najie, Zhang Yongyou, Yao Wei.

Introduction

T4 DNA ligase is derived from recombinant genetically engineered bacteria and catalyzes the phosphodiester of the 5'-P terminus and the 3'-OH terminus of adjacent DNA strands. The bond-binding reaction promotes the connection between the viscous ends or between the blunt ends. Develop a national standard for T4 DNA ligase to promote this The industrialization of class tool enzymes is of great significance. T4 DNA ligase

1 Scope

This standard specifies the technical requirements, testing methods, packaging, transportation, storage and shelf life of T4 DNA ligase. This standard applies to T4 DNA ligase isolated and purified from recombinant genetic engineering bacteria.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 191 packaging storage and transportation icon GB/T 6682 Analytical laboratory water specifications and test methods YY/T 0087 electrophoresis device YY/T 0657 medical centrifuge

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 A nucleic acid ligase that catalyzes the reaction of the 5'-P terminal and the 3'-OH terminal of a neighboring DNA strand by a phosphodiester bond The connection between the sticky ends or between the flat ends. 3.2 In 20 μL of 1×T4 DNA ligase reaction buffer, 50% of HindIII digestion can be achieved in 30 min under 16 °C reaction conditions. The amount of enzyme required for ligation of the λDNA fragment [5' end concentration of 0.12 μmol/L (300 μg/mL)] was defined as 1 unit of activity (U).

4 Technical requirements

4.1 Appearance Clarify the clear liquid without precipitation. 4.2 T4DNA ligase activity ≥8000U/mL. 4.3 Impurities Exonuclease and endonuclease should not be included. ......

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