GB/T 35904-2018 English PDF

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GB/T 35904-2018: Real-time PCR method for detection of Trichinella spiralis
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 35904-2018	119	Add to Cart	3 days	Real-time PCR method for detection of Trichinella spiralis	Valid

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Basic data

Standard ID: GB/T 35904-2018 (GB/T35904-2018)
Description (Translated English): Real-time PCR method for detection of Trichinella spiralis
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 6,679
Date of Issue: 2018-02-06
Date of Implementation: 2018-09-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 35904-2018: Real-time PCR method for detection of Trichinella spiralis

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Real-time PCR method for detection of Trichinella spiralis ICS 11.220 B41 National Standards of People's Republic of China Trichinella Real Time Fluorescent PCR Detection Method 2018-02-06 released 2018-09-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Animal Husbandry Standardization Technical Committee (SAC/TC181) centralized. This standard was drafted. Northeast Agricultural University, People's Republic of China Heilongjiang Exit Inspection and Quarantine. The main drafters of this standard. Song Xin, Zhang group, Li Yali, Han Caixia, Wang Haiyan, Li Wei, Li Xiaoyun, Lu Xinyu. Trichinella Real Time Fluorescent PCR Detection Method

1 Scope

This standard specifies Trichinella real-time fluorescence PCR detection method of operation. This standard applies to domestic animals, wild boar and dogs and other animals Trichinella nucleic acid detection.

2 The specification should reference the file

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods

3 instruments

3.1 Fluorescent PCR instrument. 3.2 high-speed refrigerated centrifuge. 3.3 biological safety cabinet. 3.4 autoclave. 3.5 Balance. precision of 0.01g or more. 3.6 tissue homogenizer. 3.7 refrigerator. 3.8 vortex mixer. 3.9 mortar. 3.10 sterile centrifuge tube. 3.11 micropipette.

4 reagent

4.1 10 × PCR Buffer buffer (no Mg2), rTaq enzyme, dNTP, Mg2, etc. All reagents are produced by biological reagents company. 4.2 The method of test water GB/T 6682 provisions of a water.

5 Control sample

5.1 Positive control samples Rat containing Trichinellaspiralis (T1) or Trichinellanativa (T2) Trichinella spiralis of 5 or more g / Meat samples. 5.2 Negative control samples After testing does not contain Trichinella rat meat samples. ......

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