GB/T 35900.1-2018 English PDF

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GB/T 35900.1-2018: Animal influenza detection -- Part 1: Method of real-time RT-PCR for the detection of H1 subtype influenza virus
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 35900.1-2018	199	Add to Cart	3 days	Animal influenza detection -- Part 1: Method of real-time RT-PCR for the detection of H1 subtype influenza virus	Valid

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GB/T 25165   NY/T 1663   GB/T 20365   GB/T 35901   GB/T 35904   GB/T 35900.3   

Basic data

Standard ID: GB/T 35900.1-2018 (GB/T35900.1-2018)
Description (Translated English): Animal influenza detection -- Part 1: Method of real-time RT-PCR for the detection of H1 subtype influenza virus
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 10,122
Date of Issue: 2018-02-06
Date of Implementation: 2018-09-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 35900.1-2018: Animal influenza detection -- Part 1: Method of real-time RT-PCR for the detection of H1 subtype influenza virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Animal influenza detection-Part 1. Method of real-time RT-PCR for the detection of H1 subtype influenza virus ICS 11.220 B41 National Standards of People's Republic of China Animal flu testing Part 1. Fluorescence of H1 subtype influenza virus nucleic acids RT-PCR detection method Animalinfluenzadetection-Part 1.Method of real-time RT-PCRforthe Published on.2018-02-06 2018-09-01 Implementation General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China China National Standardization Administration released

Foreword

GB/T 35900 Animal Influenza Test is currently divided into 3 parts. --- Part 1. Fluorescent RT-PCR detection method for H1 subtype influenza virus nucleic acid; --- Part 2. Detection of nucleic acid fluorescence of H3 subtype influenza virus by RT-PCR; --- Part 3. Double-fluorescence RT-PCR detection method for H1 and H3 subtype influenza virus nucleic acids. This part is part 1 of GB/T 35900. This section was drafted in accordance with the rules given in GB/T 1.1-2009. This part is proposed by the Ministry of Agriculture This part is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181). This section drafted by. People's Republic of China Beijing Entry-Exit Inspection and Quarantine Bureau, China Agricultural University. The main drafters of this section are. Qiao Caixia, Gu Qiang, Gao Zhiqiang, Liu Huan, Pu Jing, Zhang Hexiao, Liu Jinhua, Sun Honglei, Zhang Wei, and Zhang Lifeng.

Introduction

The issuing authority of this document draws attention to the fact that, when the statement is in compliance with this document, this document may refer to 4.1.8 and “Detection of H1 and H3 subtypes. The use of patents related to nucleotide sequences and kits of influenza viruses. The issuing agency of this document has no position on the authenticity, validity and scope of the patent. The patent holder has assured the issuing agency of this document that he is willing to apply to any applicant under reasonable and non-discriminatory terms and conditions. Negotiate on licensing of patents. The patent holder’s statement has been filed with the issuing agency of this document. Related information can be passed below Contact information is obtained. Patent holder. Beijing Entry-Exit Inspection and Quarantine Bureau, People's Republic of China. Address. 6 Tianshuiyuan Street, Chaoyang District, Beijing. Please note that in addition to the above patents, some of the contents of this document may still involve patents. The issuing agency of this document does not undertake to identify these Lee's responsibility. Animal flu testing Part 1. Fluorescence of H1 subtype influenza virus nucleic acids RT-PCR detection method

1 Scope

This part of GB/T 35900 specifies a fluorescent RT-PCR method for the detection of H1 subtype influenza virus nucleic acids. This section applies to the detection of H1 subtype influenza virus nucleic acids in swine and poultry and their products.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article Pieces. For undated references, the latest version (including all amendments) applies to this document. GB/T 6682 Analysis Laboratory Water Specifications and Test Methods GB/T 19438.1-2004 Avian influenza virus universal fluorescence RT-PCR detection method GB 19489 General Requirements for Laboratory Biosafety

3 Abbreviations

The following abbreviations apply to this document. Fluorescence RT-PCR Real-time reverse transcription polymerase chainre- (real-time reverse transcript polymerase chainre- Action) Ct (or Cp) value The number of cycles experienced by the amount of fluorescence signal in each reaction tube reaching the set threshold (cyclethreshold, or Crossingpoint) cRNA complementRNA DEPC diethylpyrocarbonate FAM carboxyfluorescein LNA locked nucleic acid (locked nucleic acid) PBS phosphate buffer solution RNA ribonucleicacid TAMRA carboxytetramethylrhodamine

4 Reagents and materials

4.1 Reagents 4.1.1 General. Unless otherwise stated, all reagents used are of analytical grade. All liquid reagents used should be packed in RNase-free containers. 4.1.2 TRIzol. Store at 2°C to 25°C. 4.1.3 Chloroform. Precooling at 2°C to 8°C. 4.1.4 Isopropyl alcohol. Precooled at -20°C. ......

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