GB/T 35029-2018 English PDF

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GB/T 35029-2018: Method based the microarray for mutation detection of hereditary hearing loss
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Basic data

Standard ID: GB/T 35029-2018 (GB/T35029-2018)
Description (Translated English): Method based the microarray for mutation detection of hereditary hearing loss
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: C30
Classification of International Standard: 11.040.55
Word Count Estimation: 8,819
Date of Issue: 2018-05-14
Date of Implementation: 2018-12-01
Regulation (derived from): National Standards Announcement No. 6 of 2018
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 35029-2018: Method based the microarray for mutation detection of hereditary hearing loss

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Genetic deafness gene detection method based on microarray chip) ICS 11.040.55 C30 National Standards of People's Republic of China Genetics based on microarray chips Deafness gene detection method Published on.2018-05-14 2018-12-01 implementation State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents. This standard is proposed and managed by the National Biochip Standardization Technical Committee (SAC/TC421). This standard was drafted. Boao Biological Group Co., Ltd. The main drafters of this standard. Jiang Di, Xiang Guangxin. Genetics based on microarray chips Deafness gene detection method

1 Scope

This standard specifies the method of detection of common hereditary deafness genes based on microarray chips. This standard applies to clinically assisted diagnosis, neonatal screening, epidemiological investigation, healthy person screening and other fields of hereditary deafness Detection of genetic loci.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB/T 27990-2011 basic terminology of biochip YY/T 1153 DNA microarray chip for in vitro diagnosis YY/T 1154 laser confocal scanner

3 Terms and definitions

The terms and definitions defined in GB/T 27990-2011 apply to this document.

4 Abbreviations

The following abbreviations apply to this document. DNA. deoxyribonucleic acid dATP. deoxyadenosine triphosphate (deoxyadenosinetriphosphate) dCTP. deoxycytidine triphosphate (deoxycytidinetriphosphate) dGTP. deoxyguanosine triphosphate (deoxyguanosinetriphosphate) dTTP. deoxythymidine triphosphate (deoxythymidinetriphosphate) GJB2. gap junction protein subunit 2 (gapjunctionbeta2) GJB3. gap junction protein subunit 3 (gapjunctionbeta3) PCR. polymerase chain reaction (polymerase chain reaction) SDS. sodium dodecyl sulfate (sodiumdodecylsulfate) SLC26A4. solute transporter 26 member 4 (solutecarrierfamily26, member4) SSC. citrate buffer solution (citratebuffersolution)

5 Principle

According to the genetic locus gene mutation site information that has been found and confirmed based on the epidemiological investigation of rickets in China, multiple Amplification and chip hybridization techniques detect genetic locus mutations commonly found in Chinese populations. ......

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