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GB/T 34799-2017 English PDF

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GB/T 34799-2017: Determination of the activity of chitinase
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
GB/T 34799-2017119 Add to Cart 3 days Determination of the activity of chitinase Valid

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Basic data

Standard ID: GB/T 34799-2017 (GB/T34799-2017)
Description (Translated English): Determination of the activity of chitinase
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: G66
Classification of International Standard: 07.080
Word Count Estimation: 6,623
Date of Issue: 2017-11-01
Date of Implementation: 2018-05-01
Regulation (derived from): National Standard Announcement 2017 No. 29
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 34799-2017: Determination of the activity of chitinase

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of the activity of chitinase ICS 07.080 G66 National Standards of People's Republic of China Chitinase activity test method Posted.2017-11-01 2018-05-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed by the National Biochemical Test Standardization Technical Committee (SAC/TC387) and centralized. This standard was drafted by Shenzhen Institute of Measurement and Quality Inspection, China Institute of Testing Technology, Sichuan Province Health and Family Planning Supervision Law Enforcement Corps, Shenzhen Polytechnic. The main drafters of this standard.Li Bifang, Lai Xin Tian, Lu Jie, Zhang Shiwei, Zhou Lihua, Liu Dong, Lin Lin, Tang Dong, Wang Shifeng, Chen Xuejian, Liu Bin, Yang Guowu. Chitinase activity test method

1 Scope

This standard specifies Serratia marcescens, Streptomyces griseus and green wood Detection of Chitinase Activity by Trichodermaviride Fermentation. This standard applies to biochemical reagents, determination of chitinase activity in industrial products.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Chitinase activity unit chitinaseactivityunit At 37 ° C, pH 6.0, β-N-acetylglucosaminidase-assisted two-step reaction catalyzed the formation of 1 mg N-B per minute The amount of chitinase in acylglucose is one unit of activity, in units of U.

4 principle

Chitinase rapidly catalyzes the hydrolysis of chitin to N-acetylglucose or disaccharides and oligosaccharides composed of N-acetylglucose, disaccharides and Oligosaccharides continue to be hydrolyzed with N-acetylglucosaminidase to N-acetylglucose, which is detectable by absorbance, Calculated N-acetyl glucose production measured enzyme activity.

5 equipment and appliances

5.1 pH meter. 5.2 electronic balance. Feel 0.0001g. 5.3 Spectrophotometer. Absorbance value accurate to 0.001. 5.4 1cm quartz cuvette. 5.5 constant temperature shaking shaker temperature control accuracy of ± 0.5 ℃.

6 Reagents

The reagents used in this standard are of analytical grade, water is in line with GB/T 6682 provisions of the two water.
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