GB/T 34795-2017 English PDF

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GB/T 34795-2017: Determination of the activity of transglutaminase
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 34795-2017	109	Add to Cart	3 days	Determination of the activity of transglutaminase	Valid

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Basic data

Standard ID: GB/T 34795-2017 (GB/T34795-2017)
Description (Translated English): Determination of the activity of transglutaminase
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: G66
Classification of International Standard: 07.080
Word Count Estimation: 5,568
Date of Issue: 2017-11-01
Date of Implementation: 2018-05-01
Regulation (derived from): National Standard Announcement 2017 No. 29
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 34795-2017: Determination of the activity of transglutaminase

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of the activity of transglutaminase ICS 07.080 G66 National Standards of People's Republic of China Detection of transglutaminase activity Posted.2017-11-01 2018-05-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed by the National Biochemical Test Standardization Technical Committee (SAC/TC387) and centralized. This standard was drafted by Shenzhen Institute of Measurement and Quality Inspection, China Institute of Testing Technology, Sichuan Province Health and Family Planning Supervision Law Enforcement Corps, Shenzhen Polytechnic. The main drafters of this standard. Zhang Shiwei, Lai Xin Tian, Lu Jie, Wang Shifeng, Zhou Lihua, Lin Lin, Dong Dong, Ye Xiuling, Lan Quan Xue, Pan Lanfang, Yang Guowu. Detection of transglutaminase activity

1 Scope

This standard specifies the source of Streptomyces mobaraensis and guinea pig (Caviaporcellus) source of ammonia Amyl transaminase activity assay. This standard applies to biochemical reagents, industrial products in the determination of transglutaminase activity.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Transglutaminase activityunit N-benzyloxycarbonyl-L-glutamylglycine (CBZ-Gln-Gly, CAS. 6610-42-0) can be catalyzed per minute at 37 ° C, The amount of enzyme required to form 1.0 μmol of hydroxamate with hydroxylamine is 1 unit in U.

4 principle

Transglutaminase can catalyze the formation of N-benzyloxycarbonyl-L-glutamyl glycine and hydroxylamine L-glutamic acid-γ-monohydroxamic acid, L-glutamine Acid-γ-monohydroxamic acid can be developed by ferric chloride, with a characteristic absorption peak at a wavelength of 525 nm. Therefore, by external standard method The activity of L-glutamic acid-γ-monohydroxamic acid was measured as the amount of enzyme produced.

5 equipment and appliances

5.1 pH meter. 5.2 electronic balance. Feel 0.0001g. 5.3 Spectrophotometer. 5.4 1cm quartz cuvette. 5.5 constant temperature water bath. Temperature control accuracy of ± 0.5 ℃. 5.6 Centrifuge.

6 Reagents

The reagents used in this standard are of analytical grade, water is in line with GB/T 6682 provisions of the two water. ......

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