GB/T 34733-2017 English PDF

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GB/T 34733-2017: Diagnostic protocols for cryptocaryonosis of marine fish
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Basic data

Standard ID: GB/T 34733-2017 (GB/T34733-2017)
Description (Translated English): Diagnostic protocols for cryptocaryonosis of marine fish
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 65.020.30
Word Count Estimation: 7,736
Date of Issue: 2017-11-01
Date of Implementation: 2018-05-01
Regulation (derived from): National Standard Announcement 2017 No. 29
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 34733-2017: Diagnostic protocols for cryptocaryonosis of marine fish

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Diagnostic protocols for cryptocaryonosis of marine fish ICS 65.020.30 B41 National Standards of People's Republic of China Marine fish to stimulate the diagnosis of cryptococcosis procedures Posted.2017-11-01 2018-05-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of this document may be patentable. The release of this document The agency does not assume responsibility for identifying these patents. This standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Aquatic Standardization Technical Committee (SAC/TC156) centralized. This standard was drafted by Fujian Provincial Institute of Freshwater Fisheries, Aquatic Animal Disease Prevention and Control Center in Fujian Province. The main drafters of this standard .Fan Haiping, Zengzhuang Zhuang, Zhuo Yuchen, Lin Yu, Wu Bin, Ma Ping, Zhong Quanfu, Wengzu Tong. Marine fish to stimulate the diagnosis of cryptococcosis procedures

1 Scope

This standard gives the marine fish to stimulate the prevalence of cryptococcosis and the main symptoms, the provisions of marine fish to stimulate the diagnosis of cryptococcosis Methods and requirements for sample collection and processing, diagnosis, confirmation of pathogens and determination of results. This standard applies to marine fish stimulate cryptococcosis diagnosis, epidemiological investigation, quarantine and monitoring.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. Aquatic animal quarantine experimental technical specifications Technical Specification for Aquatic Animal Quarantine Sampling

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Stimulate cryptocaryonosis Mature aquaculture fish Cryptocaryon irritation infection, pathological changes and the occurrence of abnormal behavior or death of a disease.

4 Reagents and equipment

4.1 seawater .1.05kg/cm2, 121.3 ℃ autoclave 15min. 4.2 0.85% saline. 4.3 distilled water. 4.4 Fixative .4% formaldehyde or 70% ethanol. 4.5 Giemsa staining solution. 4.6 optical microscope, dissecting mirror and magnifying glass (30 ×). 4.7 Anatomical plates, surgical scissors, ophthalmic forceps, scalpel, anatomic needles, pipettes. 4.8 slides, coverslips, Petri dishes. 4.9 label paper, marker.

5 prevalence and major symptoms

5.1 The prevalence Almost all mariculture fish can occur, the main hazards of large yellow croaker, oval scabies, groupers, red sea bream, snakehead snapper and catfish And so on, the harm caused by the seed than adult fish. Water temperature 10 ℃ ~ 30 ℃ can occur, the onset time is mainly from late May to mid July (water Temperature is about 22 ℃ ~ 28 ℃) and mid-September ~ late November (water temperature is about 25 ℃ ~ 19 ℃). ......

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