GB/T 34728-2017 English PDF

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GB/T 34728-2017: PCR for Mycoplasma agalactiae
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 34728-2017	199	Add to Cart	3 days	PCR for Mycoplasma agalactiae	Valid

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Basic data

Standard ID: GB/T 34728-2017 (GB/T34728-2017)
Description (Translated English): PCR for Mycoplasma agalactiae��
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 10,186
Date of Issue: 2017-11-01
Date of Implementation: 2018-05-01
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 34728-2017: PCR for Mycoplasma agalactiae

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
PCR for Mycoplasma agalactiae ICS 11.220 B41 National Standards of People's Republic of China Milk-free Mycoplasma PCR assay PCR for Mycoplasmaagalactiae Posted.2017-11-01 2018-05-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Animal Husbandry Standardization Technical Committee (SAC/TC181) centralized. This standard was drafted. Lanzhou Veterinary Institute of Chinese Academy of Agricultural Sciences. Drafters of this standard. Chu Yuefeng, Zhao Ping, Gao Pengcheng, Chen Shengli, He Ying, Zhi Zhong new. Milk-free Mycoplasma PCR assay

1 Scope

This standard specifies the non-milk mycoplasma PCR detection method of technical requirements. This standard applies to the detection of sheep (goats and sheep) clinical samples and isolation of non-mycoplasma nucleic acids in culture.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods

3 Abbreviations

The following abbreviations apply to this document. CA. Contagious Agalactia Maga. Mycoplasma agalactiae PCR. Polymerase Chain Reaction (Polymerase Chain Reaction) TAE. Nucleic Acid Running Buffer (Tris-Aceticacid-EDTA) CCU. Color change unit (Colorchangeunit) Taq enzyme Taq DNA polymerase (Taq DNA Polymerase) DNA. Deoxyribonucleic Acid dNTPs. Deoxyribonucleoside Triphosphates bp. BasePair

4 equipment

4.1 Instrument High-speed refrigerated centrifuge, PCR amplification instrument, nucleic acid electrophoresis, horizontal electrophoresis tank, constant temperature water bath, ordinary refrigerator, low temperature refrigerator (-70 ℃), Gel imaging system, autoclave, tissue homogenization equipment. 4.2 equipment (0.5 μL to 10 μL, 2 μL to 20 μL, 20 μL to.200 μL, 100 μL to 1000 μL), 0.22 μm sterile filter Sterile cotton swabs, sterile sampling containers.

5 reagent

5.1 2 × PCR reaction premix (containing Taq enzyme, dNTPs and loading buffer). 5.2 DL2000 DNA molecular weight standards. ......

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