GB/T 34331-2017 English PDF

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GB/T 34331-2017: Test method of Cucumber green mottle mosaic virus by transmission electron microscopy
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 34331-2017	279	Add to Cart	3 days	Test method of Cucumber green mottle mosaic virus by transmission electron microscopy	Valid

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Basic data

Standard ID: GB/T 34331-2017 (GB/T34331-2017)
Description (Translated English): Test method of Cucumber green mottle mosaic virus by transmission electron microscopy
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: G04
Classification of International Standard: 71.040.40
Word Count Estimation: 14,176
Date of Issue: 2017-10-14
Date of Implementation: 2018-09-01
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 34331-2017: Test method of Cucumber green mottle mosaic virus by transmission electron microscopy

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Test method of Cucumber green mottle mosaic virus by transmission electron microscopy ICS 71.040.40 G04 National Standards of People's Republic of China Cucumber green mottle mosaic virus transmission electron microscopy Detection method 2017-10-14 Published 2018-09-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released Directory Preface Ⅰ Introduction Ⅱ 1 Scope 1 2 Normative references 1 3 Terms and definitions 1 4 equipment, reagents and materials, environmental conditions 1 5 Sample 2 6 transmission electron microscopy step 4 7 serological and molecular biology verification 4 8 test results released 4 Disinfection 5 Appendix A (informative) cucumber green mottle mosaic virus related information 6 Appendix B (informative) formulations of commonly used reagents 8 Appendix C (informative) Cucumber green mottle mosaic virus electron micrograph 11

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard by the National Microbeam Analysis Standardization Technical Committee (SAC/TC38) and focal point. This standard drafting unit. Zhejiang University, Zhejiang Plant Protection Quarantine Bureau, Zhejiang Academy of Agricultural Sciences. The main drafters of this standard.Hong Jian, Li Junying, Wu Jianxiang, Zhou Xueping, Wang Rongzhou, Xie Li, Song West Jiao.

Introduction

Cucumber green mottle mosaic virus (Cucumber green mottle mosaic virus, CGMMV) is infected with watermelon, cucumber, melon, gourd Lu and other Cucurbitaceae important quarantine virus pathogens, was included in the "People's Republic of China entry phytosanitary pest list." This Viral infection caused by watermelon mottling mottling, watermelon industry poses a serious threat. The typical CGMMV is 18 nm in diameter and length Rigid rod-shaped particles of 300 nm, classified virally as belonging to the family Virgaviridae, (Tobamovirus) (Appendix A). The use of transmission electron microscopy can observe the morphological structure and distribution characteristics within the cell, is an auxiliary Important diagnostic criteria for serology and molecular biology. In order to correctly guide the cucumber green mottle mosaic virus morphological examination Test diagnostic work, it is necessary to develop a national standard transmission electron microscopy detection of the virus. Cucumber green mottle mosaic virus transmission electron microscopy Detection method

1 Scope

This standard specifies the application of transmission electron microscopy of cucumber green mottle mosaic virus Morphological detection of technical requirements and norms. This standard applies to cucumber green mottle mosaic virus inspection and identification.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. Quarantine and identification methods of cucumber green mottle mosaic virus Quarantine sampling methods for entry and exit of plants and plant products Code of Practice for the Detection of Plant Viruses

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Virus virus An intracellular parasitic pathogen that is infectious and self-replicating consisting of a nucleic acid and protein shell. 3.2 Virion virion Copy, assemble well-established mature virus particles. 3.3 Negative stained Using high-density heavy metal salt stain (such as phosphotungstic acid, uranium acetate, etc.) after staining, the tiny particles surrounded by samples in the dark back Scene shows the sample morphology and fine structure. 3.4 Ultrathin section ultrathinsection Application of ultra-thin microtome resin-embedded biological specimens cut into thin slices of 50nm ~ 100nm thickness, electronically stained for through Radio microscope observation. 3.5 Immunoelectron microscopy Using the principle of antigen-antibody-specific binding, antibodies captured by antigen capture antibody or colloidal gold as probes in transmission electron microscopy or scanning Electron microscopy at the level of positioning, qualitative and semi-quantitative display of antigen technology. 4 equipment, reagents and materials, environmental conditions 4.1 Equipment 4.1.1 Transmission electron microscope. ......

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