GB/T 34265-2017 English PDFUS$319.00 · In stock
Delivery: <= 4 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 34265-2017: Guide for the method of Sanger sequencing Status: Valid
Basic dataStandard ID: GB/T 34265-2017 (GB/T34265-2017)Description (Translated English): Guide for the method of Sanger sequencing Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: A40 Classification of International Standard: 07.080 Word Count Estimation: 16,126 Date of Issue: 2017-09-07 Date of Implementation: 2018-04-01 Quoted Standard: GB/T 6682 Regulation (derived from): National Standard Notice 2017 No. 22 Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China Summary: This standard specifies the Sanger sequencing method terms and definitions, abbreviations, principles, major equipment, Sanger gene sequencing, Sanger gene sequencing, Sanger gene sequencing applications. This standard applies to animal tissues, plant tissues, blood, oral mucosa, hair, feces, soil, microorganisms and other biological samples of DNA sequencing by Sanger sequencing method. GB/T 34265-2017: Guide for the method of Sanger sequencing---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Guide for the method of Sanger sequencing ICS 07.080 A40 National Standards of People's Republic of China Sanger sequencing technology guide 2017-09-07 Posted 2018-04-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released Directory Preface Ⅰ Introduction Ⅱ 1 Scope 1 2 Normative references 1 3 Terms and definitions 1 4 Abbreviations 2 5 principle 2 6 major equipment, equipment 2 7 Sanger gene sequencing index 3 8 Sanger gene sequencing method 4 9 Sanger method gene sequencing applications 5 Appendix A (informative) Sanger method sequencing principle diagram 6 Appendix B (informative) reagents and solution preparation 7 Appendix C (informative) Preparation of sequencing reaction system 8 Appendix D (informative) amplification program 9 Appendix E (Informative) to be sequenced product purification 10 References 11 ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed by the National Biochemical Test Standardization Technical Committee (SAC/TC387) and centralized. This standard was drafted unit. Shenzhen Huaneng Kang Gene Technology Co., Ltd., Shenzhen Huanen Kang high throughput biotechnology Research Institute, Guangzhou Kang Xin Swiss Genomics Health Technology Co., Ltd., Wuhan Kang Xin Rui Gene Health Technology Co., Ltd., China Institute of Test Technology, Shanghai Jiao Tong University Medical College Affiliated Ruijin Hospital, National Human Genome Southern Research Center, China Institute of Metrology, Sun Yat-sen University Cancer Center. The main drafters of this standard. Sheng Shitong, Tan Huibiao, Li Hua, Zeng Tao, Yang Jiebin, Zhou Lihua, Zhang Jun, Wang Sheng Yue, Quan Can, Chen Gong.IntroductionGene sequencing method is the core technology of genetic science and technology industry chain, its development status is directly related to the development of biological industry, Science, Cancer Conflict, Genetics, Pharmaceutical Research, Pharmacodynamic Analysis, Agriculture, New Energy, National Ethnic Strategic Security and Human Life Health and Safety A crucial supporting role. Gene sequencing technology in the global development is very rapid, the most widely used is the Sanger sequencing method, which is ultra-high precision The gold standard for sequencing is by far the only technology that can be used both in de novo sequencing of the human genome and in de novo assembly. will Sanger sequencing method standardization and to promote the use of biological enterprises and the entire domestic industry will get a perfect technical specifications and Protect barriers to self-development, promote the development of the industrial chain in the industry as a whole, and enhance the technical strength of the entire industry. Sanger sequencing as soon as possible The standardization of methods will play an important role in promoting the development of our country's genetics testing industry, a strategic emerging industry. for In order to make the Sanger sequencing method more standardized and effectively play its important scientific and technological support role, it is necessary to set the standard for it. Because the Sanger sequencing method is not a single technology, it involves a combination of different technical fields, including biological, chemical, mechanical, data processing Therefore, this standard only Sanger gene sequencing terms, technical indicators, as well as the instruments involved, reagents, methods are described And regulations. Sanger sequencing technology guide1 ScopeThis standard specifies the terms and definitions Sanger sequencing method, abbreviations, principles, major equipment, equipment, Sanger gene sequencing Index, Sanger gene sequencing method, Sanger method gene sequencing applications. This standard applies to animal tissues, plant tissues, blood, oral mucosa, hair, feces, soil, microorganisms and other biological samples DNA was sequenced by the Sanger sequencing method.2 Normative referencesThe following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods3 Terms and definitionsThe following terms and definitions apply to this document. 3.1 Gene sequencing genesequencing The determination of the nucleotide sequence of the nucleic acid molecules, that is, the determination of adenine (A), guanine (G), cytosine (C) And thymine (T) or uracil (U) in the order of arrangement. [GB/T 30989-2014, Definition 3.18] 3.2 Sanger sequencing method methodSangersequencing The dideoxy chain termination method used for gene sequencing is randomly blocked during the chain extension with a fluorescently labeled dideoxy base to give A, T, C, G end of the four groups of different lengths of nucleotide chain, by reading the fluorescent signal to achieve nucleic acid base sequence information read. 3.3 Denaturation denaturation The secondary or tertiary structure of a protein or nucleic acid is altered by physical or chemical factors leading to changes in its physico-chemical properties and biological activity Sexual loss phenomenon. 3.4 Primer primer In the DNA replication process, the start site and/or termination site bound to the template strand and extending as a replication has a length Degree and order of oligonucleotide chains. [GB/T 30989-2014, Definition 3.11] 3.5 Readlengthofgenesequencing The length of the longest gene fragment that can be sequenced. Note. usually expressed in terms of bases. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 34265-2017_English be delivered?Answer: Upon your order, we will start to translate GB/T 34265-2017_English as soon as possible, and keep you informed of the progress. The lead time is typically 2 ~ 4 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 34265-2017_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 34265-2017_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. 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