GB/T 32951-2016 English PDF

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GB/T 32951-2016: Determination of oxytetracycline, tetracycline, chlortetracycline and doxycycline content for organic fertilizers -- HPLC method
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 32951-2016	279	Add to Cart	3 days	Determination of oxytetracycline, tetracycline, chlortetracycline and doxycycline content for organic fertilizers -- HPLC method	Valid

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Basic data

Standard ID: GB/T 32951-2016 (GB/T32951-2016)
Description (Translated English): Determination of oxytetracycline, tetracycline, chlortetracycline and doxycycline content for organic fertilizers -- HPLC method
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: G20
Classification of International Standard: 65.080
Word Count Estimation: 14,140
Date of Issue: 2016-08-29
Date of Implementation: 2017-03-01
Regulation (derived from): National Standard Notice No. 14 of 2016
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 32951-2016: Determination of oxytetracycline, tetracycline, chlortetracycline and doxycycline content for organic fertilizers -- HPLC method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of oxytetracyline, tetracyline, chlortetracycline and doxycycline content for organic fertilizers - HPLC method ICS 65.080 G20 National Standards of People's Republic of China Organic fertilizers in oxytetracycline, tetracycline, chlortetracycline with potency Determination of the content of mycacin by high performance liquid chromatography Determination ofoxytetracyline, tetracyline, chlortetracyclineanddoxycycline 2016-08-29 released 2017-03-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China China National Standardization Management Committee released

Foreword

This standard is drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed by the China Petroleum and Chemical Industry Association. This standard is regulated by the National Technical Committee on Standardization of Fertilizers and Soil Conditioners (SAC/TC105). The drafting of this standard. Shanghai Chemical Industry Research Institute, Shanghai Entry-Exit Inspection and Quarantine Bureau of industrial products and raw materials testing technology center, Taizhou City Product Quality Supervision and Inspection Institute, Shanghai Jiaotong University. The main drafters of this standard. Zhou Hui, Zhou Yun, Sun Mingxing, business photo Cong, Ye Ping, Ma Ming, Min Hong, Shen Guoqing, Zhao Jie, Hong Liling. Organic fertilizers in oxytetracycline, tetracycline, chlortetracycline with potency Determination of the content of mycacin by high performance liquid chromatography WARNING. The use of this standard may involve hazardous materials, operations and equipment, but this standard is not intended to be relevant to all All questions are made. It is the responsibility of the user to establish safety and protective measures before use and to determine the relevant regulations. The use of the system.

1 Scope

This standard specifies the high performance liquid chromatography (HPLC) method for the determination of oxytetracycline, tetracycline, chlortetracycline and doxycycline in organic fertilizers. This standard is applicable to the determination of oxytetracycline, tetracycline, chlortetracycline and doxycycline in organic fertilizers and organic-inorganic compound fertilizers. This standard does not apply to the determination of oxytetracycline, tetracycline, chlortetracycline and doxycycline in soybean cake fertilizer. The detection limits of this method are. oxytetracycline 0.75mg/kg, tetracycline 0.75mg/kg, chlortetracycline 1.0mg/kg, strong mold The amount of 0.75mg/kg.

2 normative reference documents

The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article Pieces. For undated references, the latest edition (including all modifications) applies to this document. GB/T 6379.1 Accuracy of measurement methods and results (accuracy and precision) - Part 1. General and definitions GB/T 6379.2 Accuracy of measurement methods and results (accuracy and precision) - Part 2. Determination of standard measurement methods The basic method of sex and reproducibility GB/T 6682 Analytical laboratory water specifications and test methods

3 principle

The oxytetracycline, tetracycline, chlortetracycline and doxycycline were extracted by Na2EDTA-Mclvaine-methanol extract, and the solid phase extraction column Purification treatment after injection, high performance liquid chromatography UV detector determination, external standard quantitative.

4 reagents and materials

Unless otherwise stated, all reagents are of analytical grade and water is a primary water specified in GB/T 6682. 4.1 methanol. pure chromatography. 4.2 acetonitrile. pure chromatography. 4.3 disodium hydrogen phosphate (Na2HPO4 · 12H2O). 4.4 Citric acid (C6H8O7 · H2O). 4.5 disodium EDTA (Na2EDTA · 2H2O). 4.6 Hydrated oxalic acid (C2H2O4 · 2H2O). 4.7 disodium hydrogen phosphate solution. 0.4mol/L. Weigh 143.26g disodium hydrogen phosphate, dissolved in water, volume to 1000mL. 4.8 citric acid solution. 0.2mol/L. Weigh 42.03g citric acid, dissolved in water, set to 1000mL. ......

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