GB/T 32945-2016 English PDF

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GB/T 32945-2016: Bovine tuberculosis diagnosis -- Assay on IFN-γ in vitro
Status: Obsolete

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 32945-2016	439	Add to Cart	3 days	Bovine tuberculosis diagnosis -- Assay on IFN-γ in vitro	Obsolete

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Basic data

Standard ID: GB/T 32945-2016 (GB/T32945-2016)
Description (Translated English): Bovine tuberculosis diagnosis -- Assay on IFN-�� in vitro
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 11,182
Date of Issue: 2016-08-29
Date of Implementation: 2017-03-01
Regulation (derived from): National Standard Announcement 2016 No.14
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 32945-2016: Bovine tuberculosis diagnosis -- Assay on IFN-γ in vitro

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Bovine tuberculosis diagnosis - Assay on IFN-γ in vitro ICS 11.220 B41 National Standards of People's Republic of China In vitro detection of bovine tuberculosis by interferon - gamma method 2016-08-29 released 2017-03-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China China National Standardization Management Committee released Directory Preface I Introduction II 1 Scope 1 2 Abbreviations 1 Principle 1 4 IFN-γ in vitro assay 1 4.1 Instruments and supplies 1 4.2 Reagent 2 4.3 Procedure 2 Appendix A (normative) Reagent preparation 4 Appendix B (informative) Sampling and sample handling precautions 6

Foreword

This standard is drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. The issuer of this document does not assume responsibility for the identification of these patents. This standard is proposed by the Ministry of Agriculture of the People's Republic of China. This standard is nationalized by the National Animal Health Standardization Technical Committee (SAC/TC181). The drafting of this standard. Huazhong Agricultural University, Wuhan Branch before the animal biological products limited liability company, Beijing Entry-Exit Inspection and Quarantine Bureau, wide Animal Disease Prevention and Control Center of West Zhuang Autonomous Region. The drafters of this standard. Guo Aizhen, Chen Yingyu, Tu Lingling, Hu Changmin, Chao Jin, Ma Guiping, Hu Qiaoyun, Chen Huanchun.

Introduction

Bovine tuberculosis is mainly caused by Mycobacterium bovis (Mycobacterium bovis, M. bovis) caused by a zoonotic chronic infectious diseases, Showing a worldwide distribution. The World Organization for Animal Health (OIE) lists it as a must-report and international trade must be classified as Second-class animal disease, is the "national long-term animal disease prevention and control plan (2012 ~ 2020)" 16 kinds of priority control of the domestic major animal disease one. Clinical tuberculosis is the most common type of tuberculosis, but breast tuberculosis and intestinal tuberculosis can also occur. Healthy cattle can be contaminated by Air, feed and drinking water through the respiratory and gastrointestinal infections; In addition, cattle tuberculosis is one of the important sources of human tuberculosis. As the cattle branch Bacteria infection spectrum widely, persistent infection, cattle slow course of tuberculosis, clinical symptoms such as atypical reasons, cattle tuberculosis purification is relatively difficult. along with In recent years, China's modern dairy industry and the rapid development of beef cattle industry and domestic cattle trade increasingly frequent, China's cattle tuberculosis is on the rise. Domestic and foreign generic bovine tuberculosis control strategy is "quarantine - culling", therefore, accurate detection is the premise of control and purification of bovine tuberculosis. The current quarantine method for bovine tuberculin intradermal allergy, the method has the advantage of high sensitivity, the disadvantage is. the operation time-consuming cumbersome, knot Fruit judgment is subjective and can be cross-reactive with environmental mycobacterial infections, immunosuppressive or tuberculosis reactivity to tuberculin reduce. Bovine tuberculosis diagnosis in vitro detection of IFN-γ method is the use of bovine tuberculin and tuberculin to stimulate peripheral blood lymphocyte release IFN-γ, a method for determining the infection status of bovine tuberculosis by detecting IFN-γ concentration is a method with high sensitivity and specificity, And can achieve high-throughput detection. Developed countries have successfully used this method to assist in the diagnosis and monitoring of bovine tuberculosis. Establishment of China 's Bovine Tuberculosis Diagnosis IFN-γ in vitro test national standard, for cattle tuberculosis early diagnosis, quarantine, monitoring and epidemiological survey to provide technical support, to promote Into the control and purification of bovine tuberculosis in China, and ensure the healthy and sustainable development of cattle industry in China. In vitro detection of bovine tuberculosis by interferon - gamma method

1 Scope

This standard specifies the abbreviations, principles, reagents, sampling and operating procedures for the detection of gamma tuberculosis in vitro by the detection of bovine tuberculosis. This standard applies to the early diagnosis, quarantine, surveillance and epidemiological investigation of bovine tuberculosis.

2 abbreviations

The following abbreviations apply to this document. A-PPD. avianpurifiedproteinderivative B-PPD. bovinepurifiedproteinderivative ELISA. enzymoinked immunosorbent assay (enzymelinkedimmunosorbentassay) HRP. horseradishperoxidase IFN-γ. interferonamma IgG. immunoglobulin G PBS. phosphate buffer (phosphatebuffersolution) OD. optical density

3 principle

Mycobacterium tuberculosis positive tuberculosis, its peripheral blood lymphocytes have been Mycobacterium bovis sensitized and produce immune memory. When in vitro In the event of bovine mycobacterial specific antigen (bovine tuberculin) stimulation, lymphocytes will be activated, the occurrence of re-cellular immune response, Put IFN-γ. Mycobacterium bovis infection can be diagnosed by detecting the level of IFN-γ by sandwich ELISA. Mycobacterium tuberculosis is non-tuberculosis Mycobacterium tuberculosis, with tuberculin to stimulate lymphocytes as a control, can effectively rule out the environment caused by mycobacterial infection nonspecific reaction. 4 IFN-γ in vitro assay 4.1 Instruments and supplies 4.1.1 Class II biological safety cabinet. 4.1.2 Carbon dioxide incubator. 4.1.3 Microplate reader. 4.1.4 Mixer. 4.1.5 refrigerator. 4.1.6 balance. 4.1.7 high pressure disinfection pot. 4.1.8 trace adjustable pipette and tip. 4.1.9 Disposable sterile centrifuge tube (2 mL). 4.1.10 disposable heparin sodium anticoagulant blood collection tube. 4.1.11 Disposable syringes (5 mL). ......

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