GB/T 28084-2011 English PDF

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GB/T 28084-2011	150	Add to Cart	Auto, < 3 mins	Detection and identification of Verticillium dahliae Kleb.	Valid

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Basic data

Standard ID: GB/T 28084-2011 (GB/T28084-2011)
Description (Translated English): Detection and identification of Verticillium dahliae Kleb.
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 11,149
Date of Issue: 2011-12-30
Date of Implementation: 2012-06-01
Quoted Standard: GB 15569
Regulation (derived from): Announcement of Newly Approved National Standards No. 23 of 2011
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the symptoms of Verticillium field identification, sampling, Verticillium dahliae (Verticilliumdahliae Kleb.) Isolation and culture, detection and identification of other methods. This standard applies to cotton and cottonseed plant tissues V. dahliae quarantine detection and identification.

GB/T 28084-2011: Detection and identification of Verticillium dahliae Kleb.

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Verticillium dahliae Kleb. ICS 65.020.01 B16 National Standards of People's Republic of China Verticillium dahliae detection and identification of quarantine Issued on. 2011-12-30 2012-06-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. The main drafters of this standard. WU Li-feng, Han Shiping, Ning Hong, Bai Xiaodong, Liu Hui Chi, Lisu Fang, Xiong Hongli, Zhang Lei. Verticillium dahliae detection and identification of quarantine

1 Scope

This standard specifies the symptoms of Verticillium field identification, sampling, separating Verticillium dahliae (VerticilliumdahliaeKleb.) The Culture, detection and identification methods. This standard applies to quarantine detection and identification of plant tissues and cotton verticillium wilt of cotton and cotton seeds.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB 15569 transporting agricultural plant quarantine rules

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 Microsclerotia microsclerotium Verticillium fungus growth process by one or several separated mycelium enlargement, cell wall thickening, formed in all directions to budding meat Black eye visible mycelium particles. 3.2 Selective medium selectivemedium When plant pathogen isolation and culture, to promote the formation of colonies of the target, to inhibit the growth of other non-target organisms, added to the medium The different carbon sources, the medium having a nitrogen source or the ability to select certain inhibitory substances formed. Principle 4 Verticillium fungus mainly in the cotton plant, cotton seed, soil, disability organizations, manure, etc., after the infected cotton plants exhibited typical Type symptoms are an important basis for the field diagnosis; the use of selective media, target pathogens isolated from different contaminated materials, morphology Characterization. 5 instruments, appliances and reagents 5.1 equipment, appliances Scales, autoclaves, incubators, biological microscopes, pipettes, forceps, scalpels, vaccination rods, 9cm ~ 12cm petri dishes, beakers, Slides, cover slips, straw, clean benches and the like. 5.2 Reagents Unless otherwise indicated, the use of only standard analytical reagent. ......

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