GB/T 28075-2011 English PDF

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GB/T 28075-2011: Detection and identification of Pseudomonas savastanoi pv. phaseolicola (Burkholder) Gardan et al.
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Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 28075-2011	349	Add to Cart	3 days	Detection and identification of Pseudomonas savastanoi pv. phaseolicola (Burkholder) Gardan et al.	Valid

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Basic data

Standard ID: GB/T 28075-2011 (GB/T28075-2011)
Description (Translated English): Detection and identification of Pseudomonas savastanoi pv. phaseolicola (Burkholder) Gardan et al.
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 15,111
Date of Issue: 2011-12-30
Date of Implementation: 2012-06-01
Adopted Standard: ISTA 7-023-2008, NEQ
Regulation (derived from): Announcement of Newly Approved National Standards No. 23 of 2011
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the Sabouraud Pseudomonas beans raw Pathotype biology, serology and molecular biology detection and identification methods. This standard applies to plant seeds, seedlings and other propagating materials and products in Sabouraud Pseudomonas raw beans detection and identification of pathogenic form.

GB/T 28075-2011: Detection and identification of Pseudomonas savastanoi pv. phaseolicola (Burkholder) Gardan et al.

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Pseudomonas savastanoi pv. Phaseolicola (Burkholder) Gardan et al. ICS 65.020.01 B16 National Standards of People's Republic of China Sabouraud Pseudomonas green bean Pathotypes Quarantine and identification methods phaseolicola (Burkholder) Gardanetal. Issued on. 2011-12-30 2012-06-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. The standard reference method using redrafted International Seed Testing Association (InternationalSeedTestingAssociation, ISTA) of SeedHealthTestingMethods No. 7-023 standard "peas in Sabouraud Pseudomonas green bean Pathotype detect" the preparation, and 7-023.2008 standard non-equivalent. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. Xiamen, People's Republic of China Exit Inspection and Quarantine, People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Lin Shiming, Chen Qing, Huang Peng Ying, Yi Jianping, Liao Furong, Wu Yuan, Chen Yun, Wang Hongyi. Sabouraud Pseudomonas green bean Pathotypes Quarantine and identification methods

1 Scope

This standard specifies the Sarbanes-Oxley Pseudomonas method of detection and identification of pathogenic form of raw bean biology, serology and molecular biology. This standard applies to plant seeds, seedlings and other propagation materials and products in Savigny Pseudomonas green bean Pathotype detection and identification.

2 Sabouraud Pseudomonas green bean Pathotypes basic information

Chinese name. Sabouraud Pseudomonas green bean pathogenic type (bean halo blight). Name. Pseudomonassavastanoi pv.phaseolicola (Burkholder, 1926) Gardan, Bolet, Abu Ghorrah, Grimont Disease English. haloblightofbean Synonym. Bacteriummedicaginisvar.phaseolicola (Burkholder) Link BacteriumpuerariaeHedges, 1927 Phytomonasmedicaginis (Sackett) var.phaseolicolaBurkholder, 1926 Phytomonaspuerariae (Hedges) Bergeyetal., 1930 Pseudomonasmedicaginisvar.phaseolicola (Burkholder) Stapp Pseudomonasmedicaginisf.sp.phaseolicola (Burkholder) Dowson, 1957 PseudomonasmedicaginisBurkholder, 1926 Pseudomonasphaseolicola (Burkholder) Dowson, 1943 Pseudomonassyringaepv.phaseolicola (Burkholder) Young, Dye Xanthomonasmedicaginisvar.phaseolicola (Burkholder) Eliot, 1951 Genus Prokaryotae Procaryotes, proteobacteria door Proteobacteria, γ- Proteobacteria Gang Gammaproteobacteria, Pseudomonas Campestris head Pseudomonadales, Pseudomonas Branch Pseudomonadaceae, Pseudomonas genus Pseudomonas. Sabouraud Pseudomonas green bean Pathotype additional information, see Appendix A.

3 PRINCIPLE OF THE METHOD

Based on colony morphology of bacteria grown on media, the utilization of carbon sources, based on antigen-antibody reaction of the double-antibody sandwich enzyme-linked immunosorbent Attached assay (DAS-ELISA), based on in vitro DNA synthesis techniques of polymerase chain reaction (PCR) and wherein the pathogenic bacteria after inoculation And other detection and identification.

4 Reagents

The standard detection methods identified mainly using the following reagents. Peptone, sodium chloride, magnesium sulfate, dipotassium phosphate, sucrose, tyrosine, glycerin, boric acid, cephalexin, vancomycin, bromophenol blue, Actinobacillus (Bacteria) or Nystatin ketone, chloroform, isoamyl alcohol, isopropyl alcohol, Tris- hydrochloric acid, EDTA, SDS (sodium dodecyl sulfate), CTAB (ten Cetyl trimethyl ammonium bromide), ethidium bromide and other PCR-related reagents. ......

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