GB/T 28080-2011 English PDF

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Basic data

Standard ID: GB/T 28080-2011 (GB/T28080-2011)
Description (Translated English): Detection and identification of Tilletia indica Mitra
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 23,279
Date of Issue: 2011-12-30
Date of Implementation: 2012-06-01
Quoted Standard: GB/T 6682; GB/T 18085; GB/T 19495.2
Regulation (derived from): Announcement of Newly Approved National Standards No. 23 of 2011
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the bunt of wheat in India quarantine and identification of bacteria, including morphological and molecular detection methods, provides wheat bunt fungus quarantine and identification process in India, a clear sampling and sample preservation methods. This standard applies to wheat and processed products pass band of Indian wheat bunt fungus is detected. This standard also applies to the addition of wheat than the pass band of the host bacteria other bacteria detection bunt of wheat in India.

GB/T 28080-2011: Detection and identification of Tilletia indica Mitra

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Detection and identification of Tilletia indica Mitra ICS 65.020.01 B16 National Standards of People's Republic of China India's wheat bunt quarantine and identification of bacteria Issued on. 2011-12-30 2012-06-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Table of Contents

Preface Ⅰ 1 Scope 1 2 Normative references 1 3 Indian wheat bunt fungus basic information 1 4 principle of the method 1 5 quarantine and identification of Scheme 1 6 Sampling Method 3 6.1 unprocessed wheat sampling methods 3 6.2 Processing wheat sampling methods 3 6.3 Example 3 bags of flour sample 7 3 Main equipment and reagents 7.1 The main equipment 3 7.2 Reagents 4 8 Detection and identification of 4 Sample preparation before 8.1 4 8.2 Morphological identification 5 8.3 teliospore single direct molecular detection 5 Molecular Detection 8.4 spore culture 5 8.5 Sequencing and alignment 5 Analyzing Results 9 and Expression 5 9.1 morphological analysis showed judgment and presentation 5 9.2 single molecule detection spore direct result of the determination and presentation 5 9.3 spore cultures molecule detection result of the determination and presentation 6 9.4 Sequencing and alignment 6 10 samples and raw data storage 6 6 10.1 Sample Storage 10.2 original data saved 6 Appendix A (informative) Indian wheat bunt fungus additional information 7 Appendix B (normative) morphological identification of 9 Annex C (informative) India wheat bunt spores and bacteria and scanning microscopic morphology diagram form in FIG approximately 10 species Annex D (normative) single direct molecular detection teliospore 12 Appendix E (normative) spore culture of conventional PCR detected 14 Annex F (normative) spore culture of real-time PCR to detect 16 Annex G (normative) Sequencing and alignment 18

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. People's Republic of China, Shenzhen CIQ, Shenzhen Inspection and Quarantine Science Research Institute. The main drafters. Zhang Guiming, Cheng Yinghui, Wang Ying, Lu Qing, Yuan Ling apricot, Longhai, Chen Chi-nan, only to jade, Yang Weidong, Miao Jian Kun. India's wheat bunt quarantine and identification of bacteria

1 Scope

This standard specifies the method for inspection and identification of bacteria bunt of wheat in India, including morphological and molecular biology methods, provisions Indian wheat bunt quarantine bacteria identification process, defined the sampling and sample preservation methods. This standard applies to wheat bunt India Detection of bacteria in wheat and its processing products pass band. This standard is also applicable to other than wheat the other host bacteria pass detecting bacteria with India bunt of wheat.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 18085 phytosanitary wheat dwarf bunt quarantine and identification of bacteria GB/T 19495.2 technical requirements for GMO testing laboratories

3 Indian wheat bunt fungus basic information

Chinese name. India wheat bunt fungus. Name. Tilletiaindica Mitra. Synonyms. Neovossiaindica (Mitra) Mundkur. Disease English. karnalbuntofwheat (abbreviated KB), partialbuntofwheat. Fungi industry Fungi, Basidiomycota Basidiomycota, Ustilago Gang Ustomycetes, Ustilago mesh Ustilaginales, Tilletia Bacteria Branch Tiletiaceae, fishy Ustilago Tilletia. India's wheat bunt incidence of bacteria, mainly through seed propagation over long distances and attached to the surface of the seed teliospore health, but also with Soil and other agricultural tools to spread. For additional information, see Appendix A. Bacteria bunt of wheat in India

4 principle of the method

According to the biological and morphological characteristics of the Indian wheat bunt fungus and molecular biological characteristics, application-related instruments, including microscopes PCR instrument, such as wheat and India bunt fungus was identified.

5 quarantine and identification processes

Quarantine and identification process shown in Figure 1. ......

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