GB/T 28071-2011 English PDF

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GB/T 28071-2011: Detection and identification of cucumber green mottle mosaic virus
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 28071-2011	349	Add to Cart	3 days	Detection and identification of cucumber green mottle mosaic virus	Valid

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Basic data

Standard ID: GB/T 28071-2011 (GB/T28071-2011)
Description (Translated English): Detection and identification of cucumber green mottle mosaic virus
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 15,167
Date of Issue: 2011-12-30
Date of Implementation: 2012-06-01
Regulation (derived from): Announcement of Newly Approved National Standards No. 23 of 2011
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the cucumber green mottle mosaic virus quarantine and identification methods. This standard applies may carry cucumber green mottle mosaic virus seeds, seedlings and its products quarantine and identification.

GB/T 28071-2011: Detection and identification of cucumber green mottle mosaic virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of cucumber green mottle mosaic virus ICS 65.020.01 B16 National Standards of People's Republic of China Cucumber green mottle mosaic virus quarantine and identification methods Issued on. 2011-12-30 2012-06-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. People's Republic of China, Xiamen Entry-Exit Inspection and Quarantine of Guangdong People's Republic of China Bureau, Chinese Academy of Inspection and Quarantine. The main drafters of this standard. Chen Qing, Chen Yun, Liao Furong, Lin Shiming, Wu Bingbing, Feng Lixia, Zhang Yongjiang. Cucumber green mottle mosaic virus quarantine and identification methods

1 Scope

This standard specifies the cucumber green mottle mosaic virus quarantine and identification methods. This standard applies to quarantine and identification may carry cucumber green mottle mosaic virus seeds, seedlings and its products.

2 cucumber green mottle mosaic virus Basic Information

Chinese name. cucumber green mottle mosaic virus. Name. cucumbergreenmottlemosaicvirus. Abbreviation. CGMMV. Tobacco mosaic virus belongs to the genus Tobamovirus members. CGMMV can be spread through contact, it can also be spread by seed. Aphids spread mediator melons and other common viruses can not spread CGMMV. Grafting and other farming operations is one of the main factors of disease epidemics field. Plants Once the disease if no treatment to kill soil Soil can also be carriers of the virus, infected soil can also become the source of infection of disease occurrence. For additional information, see Appendix cucumber green mottle mosaic virus A.

3 PRINCIPLE OF THE METHOD

By immunosorbent assay (DAS-ELISA) based on antigen-antibody reaction of the double-antibody sandwich enzyme-linked, in vitro transcription and DNA synthesis in vitro Reverse transcription polymerase chain reaction to technology (RT-PCR), and detection and identification based on biological characteristics. 4 equipment, appliances, and reagents 4.1 Equipment Washer, ELISA detector, high-speed refrigerated centrifuge, electronic scales (sense of volume 0.001g), clean bench, vortex mixer, PCR Instrument, real-time PCR instrument, electrophoresis, electrophoresis tank, UV transmission instrument, isolated greenhouse, juicer, water bath. 4.2 Appliances Micropipette (0.5μL, 2μL, 10μL, 20μL, 100μL, 200μL, 1000μL), chemical PGR reaction tube and (or) 96 Photochemical PCR reaction plate, ELISA plate, a mortar. 4.3 Reagents 4.3.1 enzyme-linked assay reagent (see Appendix B). 4.3.2 RT-PCR detection reagent (see Appendix C). 4.3.3 real-time fluorescent RT-PCR detection reagent (see Appendix D). 4.3.4 biological assays (see Appendix E). ......

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