GB/T 27639-2011 English PDF

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GB/T 27639-2011: Real-time PCR method for the detection of tuberculosis pathogenic organisms
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 27639-2011	279	Add to Cart	3 days	Real-time PCR method for the detection of tuberculosis pathogenic organisms	Valid

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Basic data

Standard ID: GB/T 27639-2011 (GB/T27639-2011)
Description (Translated English): Real-time PCR method for the detection of tuberculosis pathogenic organisms
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 12,130
Date of Issue: 2011-12-30
Date of Implementation: 2012-04-01
Quoted Standard: GB/T 6682-2008; GB 19489-2008
Regulation (derived from): Announcement of Newly Approved National Standards No. 22 of 2011
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the Mycobacterium tuberculosis complex, Mycobacterium tuberculosis, Mycobacterium bovis real-time PCR detection method of the technical requirements and operational specifications. This standard applies to quick detection of bacterial cultures, blood, milk samples, sputum, tissue and organ, stool and other clinical samples of Mycobacterium tuberculosis complex, Mycobacterium tuberculosis, Mycobacterium bovis.

GB/T 27639-2011: Real-time PCR method for the detection of tuberculosis pathogenic organisms

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Real-time PCR method for the detection of tuberculosis pathogenic organisms ICS 11.220 B41 National Standards of People's Republic of China TB pathogens real-time PCR detection method Issued on. 2011-12-30 2012-04-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee Animal Epidemic Prevention (SAC/TC181) centralized. This standard was drafted. People's Republic of China Guangdong CIQ, Huazhong Agricultural University, China Inspection and Quarantine Science Research Courtyard, Beijing Science and Technology Development Co., Ltd. surplus Jiusi. The main drafters of this standard. Chen Ru, Liuzhong Yong, Yang Guohai, Guoai Zhen, Zeng Jian Bi, Han Xueqing, Gao Bo, Lin Xiangmei, Wu Xiaowei. TB pathogens real-time PCR detection method

1 Scope

This standard specifies the technical to Mycobacterium tuberculosis complex, M. tuberculosis, M. bovis real-time PCR detection method Summing practices. This standard applies to the rapid detection of bacterial cultures, blood, milk samples, sputum, tissue and organ, stool samples and other clinical Mycobacterium tuberculosis Composite group, M. tuberculosis, M. bovis.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682-2008 Analysis GB 19489-2008 General requirements for laboratory biosafety

3 Abbreviations

The following abbreviations apply to this document. Fluorescent signal cycle number reaches a set threshold when experienced. Ct value. dNTP. deoxyribonucleosidetriphosphate, deoxynucleoside triphosphates. DMSO. dimethylsulfoxide, dimethyl sulfoxide. EDTA. ethylenediaminetetraaceticacid, ethylenediaminetetraacetic acid. PBS. phosphatebuffersolution, phosphate buffer. PCR. polymerasechainreaction, polymerase chain reaction. Taq enzyme. TaqDNA polymerase. UDG enzyme. uracilDNAglycosylase, uracil DNA glycosylase. Principle 4 This method uses a standard probe TaqMan real-time PCR technology principle. The reaction system includes one pair for the amplification of DNA Primer, and a PCR product hybridized specifically with the fluorescently labeled probe. Probe 5 'end labeled with a fluorescent reporter group is called Su, 3 'end labeled quencher. When performing PCR extension reaction, Taq polymerase 5 'exonuclease activity of the 5' fluorophore from the probe Cutting down to the quencher group separate, so the instrument can detect the fluorescence signal 5 'fluorophore issued, part of PCR amplification Generating part of the fluorescent signal generated increase was accompanied. With the accumulation of amplification cycles increase, the instrument detects the fluorescent signal The reaction changes the amount of amplification product. This standard provides specific detection of Mycobacterium tuberculosis complex, M. tuberculosis and M. bovis total of four sets of real-time PCR detection The method (see Table 1). Wherein the Mycobacterium tuberculosis complex-specific real-time PCR method, respectively IS6110, IS1081 inserted gene Sequence template design specific amplification primers and probes; M. tuberculosis, M. bovis-specific real-time PCR method, respectively bacteria Species-specific genomic sequence as a template design specific amplification primers and probes. ......

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