GB/T 27634-2011 English PDF

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GB/T 27634-2011: Protocol of nucleic acid detection for infectious bursal disease (gumboro disease) virus
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 27634-2011	299	Add to Cart	3 days	Protocol of nucleic acid detection for infectious bursal disease (gumboro disease) virus	Valid

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Basic data

Standard ID: GB/T 27634-2011 (GB/T27634-2011)
Description (Translated English): Protocol of nucleic acid detection for infectious bursal disease (gumboro disease) virus
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 13,172
Date of Issue: 2011-12-30
Date of Implementation: 2012-04-01
Quoted Standard: GB/T 6682; GB/T 18088; GB 19489
Regulation (derived from): Announcement of Newly Approved National Standards No. 22 of 2011
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the infectious bursal disease virus by RT-PCR method and the real-time fluorescence RT-PCR detection method of the technical requirements and operational specifications. This standard applies to infectious bursal disease virus infection in rapid diagnosis.

GB/T 27634-2011: Protocol of nucleic acid detection for infectious bursal disease (gumboro disease) virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of nucleic acid detection for infectious bursal disease (gumboro disease) virus ICS 11.220 B41 National Standards of People's Republic of China Infectious bursal disease virus nucleic acid detection methods (Gumborodisease) virus Issued on. 2011-12-30 2012-04-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee Animal Epidemic Prevention (SAC/TC181) centralized. This standard was drafted. People's Republic of China, Shenzhen CIQ, Shenzhen Inspection and Quarantine Science Research Institute, South China Agricultural University, People's Republic of China Zhuhai Entry-Exit Inspection and Quarantine Bureau, Beijing Science and Technology Co., Ltd. surplus Jiusi. The main drafters of this standard. Qinzhi Feng, Sun Jie, Lu Kang body, flower Qun Yi, Chen Shukun, Lv Jianqiang, Yang Su, Fang Xingwang, have less spirit, Zhan Aijun, Bi Ying Zuo, Bing, Ruanzhou Xi, Zhu Yulan. Infectious bursal disease virus nucleic acid detection methods

1 Scope

This standard specifies the technical requirements for infectious bursal disease virus by RT-PCR method and real-time RT-PCR detection methods and operations For specification. This standard applies to rapid diagnosis of infectious bursal disease virus infection.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 18088 Entry and Exit Animal Quarantine sampling GB 19489 General requirements for laboratory biosafety

3 Abbreviations

The following abbreviations apply to this document. Fluorescent signal cycle number reaches a set threshold when experienced. Ct value. IBD. infectiousbursaldisease, infectious bursal disease. IBDV. infectiousbursaldiseasevirus, infectious bursal disease virus. SPF. specificpathogenfree, specific pathogen.

4 Materials and Reagents

4.1 Instruments and Supplies 4.1.1 DNA electrophoresis instrument. 4.1.2 gel imager. 4.1.3 refrigerated centrifuge (centrifugal force of 15000g up above). 4.1.4 Mixer. 4.1.5 refrigerator (2 ℃ ~ 8 ℃, -20 ℃, -80 ℃). 4.1.6 Micro adjustable pipette (10μL, 100μL, 1000μL). 4.1.7 Trace tape cartridge nozzle (10μL, 100μL, 1000μL). 4.1.8 sterilized mortar. 4.1.9 centrifuge tube. Optical 4.1.10 PCR reaction tube. 4.1.11 PCR instrument. 4.1.12 real-time PCR instrument. ......

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