GB/T 26351-2010 English PDF

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GB/T 26351-2010: Test methods of cockroach resistance to insecticides -- Test methods of insensitive acetylcholinesterase for Blattella germanica
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 26351-2010	169	Add to Cart	3 days	Test methods of cockroach resistance to insecticides -- Test methods of insensitive acetylcholinesterase for Blattella germanica	Valid

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Basic data

Standard ID: GB/T 26351-2010 (GB/T26351-2010)
Description (Translated English): Test methods of cockroach resistance to insecticides -- Test methods of insensitive acetylcholinesterase for Blattella germanica
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: C05
Classification of International Standard: 11.020
Word Count Estimation: 7,754
Date of Issue: 2011-01-14
Date of Implementation: 2011-05-01
Regulation (derived from): National Standard Approval Announcement 2011 No.129
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the German cockroach (Blattella germanica) in vivo insensitive acetylcholinesterase (AChE) detection method. This standard applies to German cockroach to organophosphate, biochemical detection of carbamate insecticides resistance.

GB/T 26351-2010: Test methods of cockroach resistance to insecticides -- Test methods of insensitive acetylcholinesterase for Blattella germanica

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Test methods of cockroach resistance to insecticides.Test methods of insensitive acetylcholinesterase for Blattella germanica ICS 11.020 C05 National Standards of People's Republic of China Detection method of cockroach resistance German cockroach - insensitive acetylcholinesterase method 2011-01-14 release 2011-05-01 implementation Ministry of Health of the People's Republic of China China National Standardization Management Committee released

Foreword

This standard is drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and centralized by the Ministry of Health of the People's Republic of China. The drafting of this standard. Beijing Municipal Center for Disease Control and Prevention, China Agricultural University, Chinese Academy of Military Medical Sciences Institute of Epidemiology. The main drafters of this standard. Zengxiao Peng, Gao Xiwu, Ma Yan, Qian Kun, Tong Ying, Liang Pei, Zhao Tongyan. Detection method of cockroach resistance German cockroach - insensitive acetylcholinesterase method

1 Scope

This standard specifies the method for the detection of insensitive acetylcholinesterase (AChE) in Blattellagermanica. This standard is applicable to the biochemical test of the resistance of Blattella germanica to organophosphorus and carbamate.

2 terms and definitions

The following terms and definitions apply to this document. 2.1 Differentiation of acetylcholinesterase Can inhibit the susceptibility of the German cockroach acetylcholinesterase activity 90% of the concentration of pesticides in mol/L or mmol/L. 2.2 F1 generation F1generation From the scene to capture the German cockroaches, after breeding the first generation.

3 test insects

Field Blattella germanica, indoor breeding, F1 generation after emergence 7d ~ 15d male German cockroaches. Ice bath under the conditions of storage does not exceed 1d, -20 ℃ under the conditions of storage is not more than 1 week or -80 ℃ under the conditions of storage within 6 months.

4 reagent

4.1 disodium hydrogen phosphate (Na2HPO4), chemically pure. 4.2 potassium dihydrogen phosphate (KH2PO4), chemically pure. 4.3 Triton X-100 (TritonX-100). 4.4 Acetylchiocholine (ATCh), analytical grade. 4.5 5,5'-dithiobisobenzoic acid (DTNB), analytical grade. 4.6 Phosphoric acid buffer (0.1 mol/L, pH 7.5 PBS). Weigh 30.18 g of Na2HPO4 · 12H2O and 2.16 g of KH2PO4, Water volume to 1000mL. 4.7 DTNB-ethanol reagent. Weigh 12.4mgDTNB, dissolved in 125mL 95% ethanol, add 50mL phosphate buffer, the most After the volume of distilled water to 250mL, 4 ℃ dark preservation. 4.8 ATCh solution. Weigh 28.9mgATCh dissolved in 10mL distilled water, -20 ℃ dark preservation. 4.9 ATCh-DTNB mixed solution. Weigh 75mgATCh and 13mgDTNB, dissolved in 100mL phosphate buffer. ......

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