GB/T 26349-2010 English PDF

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GB/T 26349-2010: Test methods of fly resistance to insecticides -- Test methods of insensitive acetylcholinesterase for musca domestica
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 26349-2010	169	Add to Cart	3 days	Test methods of fly resistance to insecticides -- Test methods of insensitive acetylcholinesterase for musca domestica	Valid

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Basic data

Standard ID: GB/T 26349-2010 (GB/T26349-2010)
Description (Translated English): Test methods of fly resistance to insecticides -- Test methods of insensitive acetylcholinesterase for musca domestica
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: C05
Classification of International Standard: 11.020
Word Count Estimation: 7,715
Date of Issue: 2011-01-14
Date of Implementation: 2011-05-01
Regulation (derived from): National Standard Approval Announcement 2011 No.127
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the housefly (Musca domestica) insensitive acetylcholinesterase (ACNE) detection method. This standard applies to houseflies to organophosphate, biochemical detection of carbamate insecticides resistance.

GB/T 26349-2010: Test methods of fly resistance to insecticides -- Test methods of insensitive acetylcholinesterase for musca domestica

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Test methods of fly resistance to insecticides. Test methods of insensitive acetylcholinesterase for musca domestica ICS 11.020 C05 National Standards of People's Republic of China Flies resistance detection method Housefly insensitive acetylcholinesterase law Issued on. 2011-01-14 2011-05-01 implementation People's Republic of China Ministry of Health Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given in drafting the rules. This standard is proposed and administered by the People's Republic of China Ministry of Health. This standard was drafted. China Agricultural University, Beijing Center for Disease Control and Prevention, the PLA Military Academy of Medical Sciences microorganisms Institute of Epidemiology matter. The main drafters of this standard. GAO Xi Wu, Zeng Peng, Pei Liang, Ma Yan, Ting, Tong Ying, Zhao Tong statement. Flies resistance detection method Housefly insensitive acetylcholinesterase law

1 Scope

This standard specifies the housefly (Muscadomestica) insensitive acetylcholinesterase (AChE) detection method. This standard applies to housefly biochemical detection of organophosphate, carbamate insecticides resistant.

2 Terms and definitions

The following terms and definitions apply to this document. 2.1 Acetylcholinesterase distinction concentration discriminatingconcentrationofacetylcholinesterase Concentration sensor capable of inhibiting AChE of Musca domestica 90% of the normal activity of the insecticide, in units of mol/L or mmol/L. 2.2 F1 generation F1generation From the scene captured housefly breeding to get the first generation.

3 test insects

Housefly site collection, kept indoors, after the emergence of F1's female houseflies 3d ~ 5d. Under ice storage of up to 1d, -20 ℃ storage conditions at no more than 1 week or -80 ℃ storage conditions within six months.

4 Reagents

4.1 Disodium hydrogen phosphate (Na2HPO4), chemically pure. 4.2 potassium dihydrogen phosphate (KH2PO4), chemically pure. 4.3 pull-through X-100 (TritonX-100). 4.4 acetylthiocholine (ATCh), AR. 4.5 5,5'-dithiobis-nitrobenzoic acid (DTNB), AR. 4.6 phosphate buffer (0.1mol/L, pH7.5PBS). Weigh 30.18gNa2HPO4 · 12H2O and 2.16gKH2PO4, distillation Water volume to 1000mL. 4.7 DTNB- ethanol reagent. Weigh 12.4mgDTNB, dissolved 125mL95% ethanol, 50mL phosphate buffer, most After the distilled water volume to 250mL, 4 ℃ stored. 4.8 ATCh solution. Weigh 28.9mgATCh dissolved in 10mL distilled water, -20 ℃ stored.

5 spectrophotometry

5.1 Equipment 5.1.1 spectrophotometer. ......

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