GB/T 25879-2010 English PDF

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GB/T 25879-2010: Determination of lysozyme in chicken egg white -- Spectrophotometry
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 25879-2010	169	Add to Cart	3 days	Determination of lysozyme in chicken egg white -- Spectrophotometry	Valid

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Basic data

Standard ID: GB/T 25879-2010 (GB/T25879-2010)
Description (Translated English): Determination of lysozyme in chicken egg white -- Spectrophotometry
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B43
Classification of International Standard: 65.020.30
Word Count Estimation: 7,721
Date of Issue: 1/10/2011
Date of Implementation: 6/1/2011
Quoted Standard: GB/T 6682
Regulation (derived from): Announcement of Newly Approved National Standards 2011 No. (No. 166 overall) 1
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This Standard specifies spectrophotometric method eggs egg white lysozyme. This Standard is applicable to eggs and egg white lysozyme determination of viability.

GB/T 25879-2010: Determination of lysozyme in chicken egg white -- Spectrophotometry

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of lysozyme in chicken egg white. Spectrophotometry ICS 65.020.30 B43 National Standards of People's Republic of China Determination of egg white lysozyme Issued on. 2011-01-10 2011-06-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

The standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Standardization Technical Committee of animal husbandry. This standard was drafted. Yangzhou University College of Animal Science and Technology. The main drafters of this standard. Wang Jinyu, Xie Kai Zhou, Dai Guojun, Hou Qirui, Liu Dalin. Determination of egg white lysozyme

1 Scope

This standard specifies the spectrophotometric method of egg white lysozyme. This standard applies to the determination of lysozyme in egg white content and vitality to.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. Laboratory use specifications and test methods GB/T 6682 Analysis

3 Terms and Definitions

The following terms and definitions apply to this standard. 3.1 Egg eggwhite Located in the semi-fluid colloidal substances egg yolk and eggshell endometrial film. 3.2 Lysozyme lysozyme A special effect on the microbial cell walls of glycoside hydrolases.

4 Determination of the content of egg white lysozyme

4.1 Principle Lysozyme maximum absorption peak at 281nm wavelength. Lysozyme standard solution containing 9g per liter of sodium chloride solution to dissolve and dilute Interpretation into different gradient concentration, absorbance was measured at a wavelength of 281nm and standard curve obtained standard curve regression equation. After the sample Processing, by lysozyme standard measuring method of measuring the absorbance of the sample, the sample is calculated from the standard curve regression equation based on the absorbance value Lysozyme content. 4.2 Instrument 4.2.1 UV - visible spectrophotometer (wavelength range of 190nm ~ 900nm, wavelength uncertainty ± 0.3nm, wavelength repeatability ± 0.1nm). 4.2.2 electronic analytical balance (inductance 0.1mg, 1mg). 4.2.3 Centrifuge (maximum centrifugal force of 2325g, a 10mL centrifuge tube). 4.3 Reagents and materials 4.3.1 lysozyme standard, pure class distinctions, vitality 20000U/mg or so. 4.3.2 Sodium chloride, analytical grade. 4.3.3 of distilled water, according to GB/T 6682 execution. 4.3.4 medical gauze. 4.4 reagent preparation 4.4.1 0.9% sodium chloride solution. Weigh sodium chloride (4.3.2) 0.9g, was dissolved in distilled water and dilute to 100mL. ......

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