GB/T 24903-2010 English PDF

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GB/T 24903-2010: Inspection of grain and oils -- Determination of resveratrol in peanut by high performance liquid chromatography
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 24903-2010	139	Add to Cart	3 days	Inspection of grain and oils -- Determination of resveratrol in peanut by high performance liquid chromatography	Valid

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Basic data

Standard ID: GB/T 24903-2010 (GB/T24903-2010)
Description (Translated English): Inspection of grain and oils -- Determination of resveratrol in peanut by high performance liquid chromatography
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B33
Classification of International Standard: 67.200.20
Word Count Estimation: 6,610
Date of Issue: 2010-06-30
Date of Implementation: 2011-01-01
Quoted Standard: GB/T 6682
Regulation (derived from): National Standard Approval Announcement 2010 No.2 (Total No.157)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the principles of HPLC peanut resveratrol content, reagents and materials, instruments and equipment, operating procedures and the results of calculations. This standard applies to peanuts, determination of resveratrol content of peanut. Resveratrol sample detection limit 0. 1MG/KG.

GB/T 24903-2010: Inspection of grain and oils -- Determination of resveratrol in peanut by high performance liquid chromatography

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Inspection of grain and oils. Determination of resveratrol in peanut by high performance liquid chromatography ICS 67.200.20 B33 National Standards of People's Republic of China Inspection of grain and peanut Determination of resveratrol High Performance Liquid Chromatography Issued on. 2010-06-30 2011-01-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard is an informative annex. The standard proposed by the National Food Authority. This standard by the National Standardization Technical Committee OILS. This standard was drafted. Nanjing University of Finance and Economics, Jiangnan University. The main drafters of this standard. Wang Haifeng, Yuan Jian, Yang Xiaorong, ZHANG Lian-fu. Inspection of grain and peanut Determination of resveratrol High Performance Liquid Chromatography

1 Scope

This standard specifies the principles of HPLC content of resveratrol in peanuts, reagents and materials, equipment and facilities, the operation step And the result of the calculation procedure. This standard applies to peanuts, peanut Determination of resveratrol content. Resveratrol sample detection limit of 0.1mg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. Laboratory use specifications and test methods GB/T 6682 Analysis Principle 3 Resveratrol in the sample with ethanol - water extract, the extract after centrifugation, the supernatant was equipped with a UV detector of high performance liquid chromatography Spectrometer were measured in 32 min.

4 Reagents and materials

Unless otherwise specified, the reagents were of analytical grade, test water should be consistent with GB/T 6682 in Class II. 4.1 ethanol. 4.2 Methanol. chromatographically pure. 4.3 acetonitrile. chromatography. 4.4 acetic acid. 4.5 85% ethanol solution. Take 850mL ethanol (4.1), add 150mL of water, and mix. 4.6 liquid mobile phase. acetonitrile aqueous acetic acid = 25 75 0.09. Take 250mL acetonitrile (4.3), was added 750mL of water and 0.9mL glacial acetic acid (4.4) and mix through 0.2μm membrane filter (5.6) and degassed. Resveratrol 4.7 standard. Purity ≥99%. 4.8 resveratrol standard stock solution. weigh accurately 12.5mg (accurate to 0.0001g) Resveratrol standard (4.7), methanol (4.2) to dissolve and dilute to 250mL, to give 50mg/L resveratrol standard stock solution stored in the dark at 4 ℃ refrigerator spare. 4.9 resveratrol standard solution. accurate Pipette 1mL, 2mL, 4mL, 6mL, 8mL, 10mL resveratrol stock standard solution (4.8), diluted with methanol (4.2) and set the volume to 50mL, to give a series of standard working solution (mass concentration of 1mg/L, 2mg/L, 4mg/L, 6mg/L, 8mg/L, 10mg/L). 5. Apparatus 5.1 High performance liquid chromatograph. with a UV detector. 5.2 mill. high-speed grinder, speed 24000r/min, or equivalent device. 5.3 desktop centrifuge. not less than 5000r/min, or equivalent device. ......

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