GB/T 24800.7-2009 English PDF

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GB/T 24800.7-2009: Determination of brucine and strychnine in cosmetics by high performance liquid chromatography method
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 24800.7-2009	229	Add to Cart	3 days	Determination of brucine and strychnine in cosmetics by high performance liquid chromatography method	Valid

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Basic data

Standard ID: GB/T 24800.7-2009 (GB/T24800.7-2009)
Description (Translated English): Determination of brucine and strychnine in cosmetics by high performance liquid chromatography method
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: Y42
Classification of International Standard: 71.100.70
Word Count Estimation: 10,138
Date of Issue: 2009-11-30
Date of Implementation: 2010-05-01
Quoted Standard: GB/T 6682
Regulation (derived from): National Standard Approval Announcement 2009 No.14 (Total No.154)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard provides high-performance liquid chromatographic method for cosmetic rather brucine and disabilities. This standard applies to the determination of cosmetics rather brucine and disabilities.

GB/T 24800.7-2009: Determination of brucine and strychnine in cosmetics by high performance liquid chromatography method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of brucine and strychnine in cosmetics by high performance liquid chromatography method ICS 71.100.70 Y42 National Standards of People's Republic of China Determination of cosmetics Ning brucine and strychnine High Performance Liquid Chromatography Posted 2009-11-30 2010-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

The Standard Appendix A, Appendix B is an informative annex. The standard proposed by China National Light Industry Council. This standard by the National Standardization Technical Committee of Fragrance Flavor and Cosmetic (SAC/TC257) centralized. This standard is drafted by. China Inspection and Quarantine Science Research Institute, Shanghai Daily Chemical Industry Research Institute, Shanghai Institute of spices. The main drafters of this standard. Ma Qiang, Xiao Haiqing, Wang Chao, Wang Xing, Fan Min, Julie, Cui Jian Jie, Li Qiong.

Introduction

The standard test substance is banned substances of "Hygienic Standard for Cosmetics" shall consist of not as a cosmetic raw material, component Added to cosmetics. If technically unavoidable banned substances as impurities into cosmetics, the finished cosmetic products should follow the "Cosmetics Health standards "General requirements for cosmetics, that is, under normal and reasonably foreseeable conditions of use, should not cause harm to human health. At present, China has not yet specified the substance of limited value, the present standards, the determination of these substances in cosmetics only provide detection methods. Determination of cosmetics Ning brucine and strychnine High Performance Liquid Chromatography

1 Scope

This standard specifies a high performance liquid chromatographic method Ning cosmetics brucine and strychnine. This standard applies to the determination of cosmetics rather brucine and strychnine. The standard detection and quantitation limits. brucine and strychnine detection limit of 2.5mg/kg, the limit of quantification for the 8mg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD) Principle 3 Sample by solvent extraction and centrifugal filtration, HPLC, external standard, liquid chromatography - mass spectrometry confirmed.

4 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade water as a water GB/T 6682 regulations. 4.1 Methanol. chromatographically pure. 4.2 tetrahydrofuran. chromatography. 4.3 ammonium formate solution (0.01mol/L). Weigh accurately 0.6306g ammonium formate in 1L flask, add water to dissolve about 980mL, PH was adjusted to 3.0 with formic acid, the volume to 1L spare. 4.4 methanol aqueous solution. accurate amount of 64mL of methanol and 36mL water, mix and reserve. 4.5 brucine and strychnine standards. purity of not less than 97%. Stock standard solution 4.6 brucine and strychnine. Weigh accurately brucine and strychnine standard material each 100mg, were placed 100mL brown volumetric flask, and dissolved in methanol to volume, shake, formulated to concentrations of 1000μg/mL standard stock Liquid stored in the dark at 4 ℃, can be used for three months. 4.7 mixed standard stock solution of brucine and strychnine. accurate Pipette stock standard solution of brucine and strychnine were each 25mL In 50mL brown volumetric flask with methanol to volume, the concentration of the solution rather brucine and strychnine were 500μg/mL. 5. Apparatus 5.1 High Performance Liquid Chromatography (HPLC) instrument. with a UV detector or diode array detector. 5.2 High performance liquid chromatography - mass spectrometry (LC-MS/MS) instrument. with electrospray ionization (ESI). 5.3 Analysis of balance. a sense of the amount of 0.0001g and 0.001g. 5.4 centrifuge. speed of not less than 5000r/min. 5.5 ultrasonic bath. 5.6 plug cuvette. 10mL. 5.7 plug plastic centrifuge tube. 10mL. ......

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